Cntn1 (NM_007727) Mouse Untagged Clone

CAT#: MC201930

Cntn1 (untagged) - Mouse contactin 1 (Cntn1), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cntn1
• Synonyms: AW495098; CNTN; F3cam; usl
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC066864 sequence for NM_007727
  GTCGCTATGTGGAGATCCAGCTTTGCAGTCTTATAACCTGTTCTTTGCAGAAGGTTGGCTGAAGGATGGG AGCGGCTATGCCTGCCTCTGAGGTCACAGCATGAGGTCCTTTTAACTTGCCTTCTGCTGCAGAAGAGAAG CCCATTGTGTCATAACTTTCCCTGAGTCTATTCAAAGGGGCAATTTGAAGCAGATATTATAGCAAGATCG GCAGATGATTCAACCCACACGGTCTTTCATAGTACTCAACTGCCATAAAGCTTTCATTACTTTTGGAAAC TGACCCATCTATTCAACAAAAGATGAAAATGCCATTGCTGGTCAGCCATCTCCTGCTAATCTCCTTAACA TCTTGTCTAGGAGACTTTACCTGGCACAGAAGATATGGTCATGGAGTGTCTGAGGAGGACAAAGGATTTG GGCCCATTTTTGAAGAGCAGCCAATCAATACCATTTACCCAGAAGAGTCACTGGAAGGAAAAGTGTCACT CAACTGCCGGGCACGCGCCAGCCCATTTCCAGTTTACAAATGGAGAATGAATAACGGGGACGTGGACCTG ACAAACGATCGGTACAGTATGGTCGGAGGAAACCTTGTTATCAACAACCCGGACAAACAGAAAGACGCTG GGGTGTACTACTGCCTGGCATCCAACAACTACGGAATGGTCAGGAGCACGGAGGCAACTCTGAGCTTCGG GTATCTCGACCCCTTTCCTCCCGAGGAGCGTCCGGAAGTGAAGGTGAAGGAAGGGAAGGGCATGGTGCTT CTCTGTGATCCTCCCTACCACTTCCCAGATGATCTTAGCTACCGCTGGCTTCTGAATGAATTTCCTGTAT TTATCACCATGGATAAGCGAAGATTTGTGTCTCAGACCAACGGCAATCTCTATATCGCAAATGTCGAGTC TTCTGACAGAGGCAACTACTCCTGCTTTGTGTCCAGTCCTTCTATTACAAAGAGTGTGTTCAGCAAGTTT ATTCCCCTCATTCCGATTCCTGAACGAACAACAAAGCCATATCCTGCTGATATTGTGGTGCAGTTCAAGG ACATATACACCATGATGGGCCAAAATGTGACTTTAGAGTGTTTCGCTCTAGGGAACCCTGTTCCTGATAT CCGGTGGCGGAAGGTGCTAGAACCAATGCCCAGCACTGCCGAGATAAGCACGTCAGGGGCTGTCCTCAAG ATCTTCAACATCCAGCTAGAAGATGAAGGCCTCTACGAGTGTGAGGCTGAGAACATCCGGGGAAAGGACA AGCACCAGGCAAGAATCTATGTTCAAGCGTTTCCTGAGTGGGTAGAGCATATCAATGACACTGAGGTGGA CATAGGCAGTGACCTCTACTGGCCTTGCATAGCCACAGGGAAGCCCATTCCTACCATCAGGTGGCTGAAA AATGGATACTCGTATCACAAAGGGGAACTGAGACTGTATGATGTGACTTTTGAAAATGCCGGGATGTACC AGTGCATAGCAGAAAACGCGTATGGGTCCATTTATGCCAACGCTGAGCTCAAGATCCTGGCTTTAGCTCC AACTTTTGAAATGAATCCTATGAAGAAAAAGATCTTGGCTGCGAAAGGTGGAAGGGTTGTCATTGAATGT AAACCCAAGGCCGCACCAAAACCCAAGTTTTCATGGAGCAAAGGGACAGAGTGGCTCGTCAACAGCAGCA GAATACTCATATGGGAAGATGGTAGCTTGGAAATCAATAATATTACAAGAAATGATGGTGGAATCTATAC ATGCTTTGCAGAAAATAACAGAGGAAAAGCTAATAGCACTGGGACTCTGGTGATCACAAATCCCACACGA ATTATTTTGGCCCCAATCAATGCTGACATCACTGTTGGGGAAAATGCCACCATGCAGTGTGCTGCGTCTT TCGATCCTGCCTTGGACCTCACGTTTGTGTGGTCCTTCAATGGCTACGTGATTGACTTTAACAAGGAAAT TACGCATATCCATTACCAGAGGAATTTTATGCTCGACGCCAATGGGGAACTGCTCATCCGAAATGCGCAG CTGAAACACGCAGGCCGGTACACGTGCACGGCGCAGACAATCGTGGACAATTCCTCGGCTTCCGCTGACC TTGTCGTTCGAGGCCCCCCAGGTCCCCCAGGAGGCCTGAGAATAGAAGACATCAGAGCTACCTCTGTGGC ACTGACTTGGAGCCGTGGTTCCGACAATCACAGCCCTATCTCTAAATACACGATCCAGACCAAGACGATT CTCTCAGATGACTGGAAAGATGCGAAGACAGATCCCCCAATCATTGAAGGGAACATGGAGTCAGCTAAAG CAGTGGATTTGATCCCTTGGATGGAGTATGAATTTCGAGTGGTGGCGACCAATACTTTAGGGACGGGAGA GCCCAGCATACCCTCAAACAGGATCAAAACAGACGGTGCTGCACCAAATGTGGCTCCCTCAGATGTAGGC GGTGGGGGCGGAACCAACAGAGAGCTGACAATAACGTGGGCGCCTTTGTCGAGAGAGTACCATTACGGTA ACAATTTTGGTTACATAGTGGCGTTTAAGCCATTTGATGGAGAAGAGTGGAAGAAAGTGACTGTGACTAA CCCAGACACTGGCCGCTACGTCCACAAAGATGAGACCATGACCCCCTCCACCGCGTTTCAAGTCAAAGTG AAAGCCTTCAACAATAAAGGAGATGGGCCCTACAGCCTCGTAGCTGTCATCAACTCTGCACAAGATGCTC CCAGTGAAGCCCCCACAGAGGTAGGGGTCAAAGTGTTATCATCCTCTGAGATATCTGTCCATTGGAAGCA TGTGTTAGAGAAAATTGTGGAAAGCTATCAGATTCGGTACTGGGCTGGGCATGACAAAGAAGCAGCCGCA CACAGAGTTCAGGTCACCAGCCAGGAGTACTCAGCCAGACTGGAGAACCTTCTTCCCGACACCCAGTACT TCATTGAAGTGGGAGCCTGCAACAGTGCTGGATGCGGACCTTCCAGTGATGTGATCGAGACCTTCACCAG AAAAGCCCCTCCTAGCCAACCACCAAGGATCATCAGTTCGGTGAGATCTGGCTCACGATATATAATCACA TGGGATCACGTTGTGGCACTATCCAATGAATCTACTGTGACAGGCTACAAGATACTCTATAGACCGGATG GCCAACATGACGGCAAGCTGTTCTCAACCCATAAACACTCCATAGAAGTCCCCATCCCCAGAGACGGAGA GTATGTTGTCGAGGTTCGAGCACACAGCGACGGGGGAGACGGAGTTGTGTCTCAAGTCAAAATTTCAGGC GTGTCCACGCTGTCTTCGAGCCTCCTCAGCTTGCTCCTGCCCTCCCTTGGCTTTCTTGTCTACTCGGAAT TCTGAATGTTACGACCTTGGCTGTTTTCATCCCTGCTCAGAGGATGCTCTTCATCATGGGATGACTGTAA TTCCTTCTAATCCCTGCGGCTCCATCCTAAGCCAAACAAATTAGACTCTAACAAGCTGTTCAACTGATTT CCCAACACATCACGACTGAGGCATTCAGGAACCCCTTCATCCAAAAGAATAACTTTCAAGTGGAAATAAA GTGATTTTTAACTCGTTCCAATATGCCTTATAAACCATTTAACCTGATTCTGTGAAAGTTGCATGACTTA AATCAATGGACAAGTTACAGTGTTCAATTCAATGCCCTAGGCTGTAGAGTGAAGGTCACCATACACAGGT GCTTTACATGTTTGACAAGTTGTGAAATATAATAGAGATTGAAATGTTGATTGTATGTAGTATGTGTTCG AGTAATGCAGTCTCTTGTATTAGCCTATATGTTTGGGTTCCTGCCTATTTTTGAAAGACCCTCATCATTC ATGATATTTTGAGTTTTCCATAAAATTAAAACAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_007727
• Insert Size: 3063 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC066864, AAH66864
• RefSeq Size: 3897 bp
• RefSeq ORF: 3063 bp
• Locus ID: 12805
• UniProt ID: P12960
• Cytogenetics: 15 46.39 cM
• Gene Summary: Contactins mediate cell surface interactions during nervous system development. Involved in the formation of paranodal axo-glial junctions in myelinated peripheral nerves and in the signaling between axons and myelinating glial cells via its association with CNTNAP1. Participates in oligodendrocytes generation by acting as a ligand of NOTCH1. Its association with NOTCH1 promotes NOTCH1 activation through the released notch intracellular domain (NICD) and subsequent translocation to the nucleus. Interaction with TNR induces a repulsion of neurons and an inhibition of neurite outgrowth.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) represents use of an alternate promoter, compared to variant 1. Variants 1, 2, 3, and 4 all encode the same protein.