Lefty2 (NM_177099) Mouse Untagged Clone

CAT#: MC201921

Lefty2 (untagged) - Mouse left-right determination factor 2 (Lefty2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Lefty2
• Synonyms: 6030463A22Rik; Eba; Ebaf; Left; Lefta; Leftb; lefty-2; lefty-B
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC066224 sequence for NM_177099
  GTCCCAAGAACTTTTCAGGGCACTTTTAGGGACGCATATATCCACGATTCCTCCTGGGCAGCGCCATGAA GTCCCTGTGGCTTTGCTGGGCACTCTGGGTACTGCCCCTGGCTGGCCCTGGGGCAGCGATGACCGAGGAA CAGGTCCTGAGCAGTCTACTGCAGCAGCTGCAGCTCAGCCAGGCCCCCACCCTGGACAGCGCGGATGTGG AGGAGATGGCCATCCCTACCCACGTGAGGTCCCAGTATGTGGCCCTGCTGCAGGGAAGTCACGCTGACCG CTCCCGAGGCAAGAGGTTCAGCCAGAATTTTCGAGAGGTGGCAGGCAGGTTCCTGATGTCAGAGACCTCC ACTCACCTGCTAGTGTTCGGAATGGAGCAGCGGCTGCCGCCTAACAGCGAGCTGGTGCAGGCTGTGCTGC GGCTGTTCCAGGAGCCTGTGCCCAGAACAGCTCTCCGGAGGTTTGAGAGGCTGTCCCCACACAGTGCCCG GGCTCGGGTCACCATTGAATGGCTGAGAGTCCGTGAGGATGGCTCCAATCGCACTGCCCTCATCGACTCT AGGCTCGTGTCCATCCACGAGAGCGGCTGGAAGGCCTTCGACGTGACCGAGGCCGTGAACTTCTGGCAGC AGCTGAGCCGGCCGAGGCAGCCGCTGCTGCTCCAGGTGTCGGTGCAGAGGGAGCATCTGGGGCCGGGGAC CTGGAGCGCACACAAGTTGGTCCGTTTCGCGGCGCAGGGGACGCCGGACGGCAAGGGGCAGGGCGAGCCA CAGCTGGAGCTGCACACGCTGGACCTCAAGGACTACGGAGCTCAAGGCAATTGTGACCCCGAGGTACCAG TGACTGAAGGCACCCGATGCTGTCGCCAGGAGATGTACCTGGACCTGCAGGGGATGAAGTGGGCCGAGAA CTGGATCCTAGAACCGCCAGGGTTCCTGACGTATGAATGTGTGGGCAGCTGCCTGCAGCTACCAGAGTCC CTGACCATCGGGTGGCCATTTCTGGGGCCTCGGCAGTGTGTTGCCTCAGAGATGACCTCCTTGCCCATGA TTGTCAGTGTGAAGGAGGGAGGCAGGACCAGGCCTCAAGTGGTCAGCCTGCCCAACATGAGGGTGCAGAC CTGTAGCTGCGCCTCAGATGGGGCGCTCATACCCAGGGGGATAGATCTGTAGTCTCCCTGTCCACAGATG TATTCTCAGTGAGCTTGTCCTAACTTAGTGCTCTCGTCAGACCTTTGCTCTACAGTCTTGGTTTTCTTGT CCATCACCCAGTTTAAGCACTTACATGGGTAAATCATGTCACTCCAGTAGGACACACTGACCCCACTTAG CCAAGGACATGGCTATGCAGTGAACAGGTTCGCATCTGAGTCTGTTTTCTGGCCAGAACTCAGCTTAATG TACAACAAAACCCTACGGTGAGAACAGGGGAATCAAAAGCTCGTTTACTCTTACACCGTGATTACTGGCA TCAACGTACCATGTCAGGGACTGCCCACAGCAGGCTGGGAGGGAGACATCTCAGAAGCCTGCGGCAGCTC CTTGTGAAAAACCGTTGTTCCCATTTCTCCTAACCTTAGCCCTAGACAAGAGCTGTATAGATTTCATGTG TGTGACTGCTTTTCAGTTGGCCTTGGTGTTCATAGTTATTCTATATTATTTGACTTTCCTACTCCTTTCT CCTTCTGCCCTGGTGAATTCTATGAAACTAGATGTTCCTTGATGTAATGATTCTTAAACAATTAAAAAGT TGAGGCATGGGACACAGCACAGCACAGTCCTGATGGCCCAGGTGCATGCTGTAGATGTATTCTGTGTGCT CTTATCTTGGAAACAATGCAATAACTTTGCAATGTTAGTTCAGATTAATGTTTGACTTGCAAAGAAAGTT TGAAGAAATTATTAGAAAGTGAAATAGAGCCAACACTGGGATCCCGAAAAGAAAAAAGCTATTGAAGTTA TGAAATAAGTTTTGCACAAAATTTGAGAGTGTTTCCTGGATAAGCAAGTATAGAATACATAAAATCTTAT ATTAGTAAAACTAAGCCAAAACACCGGGACTCTTAGGAGGGTCACTGCGTGCAATGTGCAGAAGCAGAAA GCTGGCAGAACTGCCGAGTTAAGGGTGTACCTGAGTCTTTCTGGCCATTGCCTGGCAGCTTTGCCCATGT CATTTATTGTCAGAGCTTCACGGGAAAATGCAAGTAGCCGACTTCGGAGCTCTGAGCTCTGGAGTATAAT AAGTCAAAAGGTAAAGTTTAAATAATGATAAGTTTGCAATAATTATTATTTTGGCCAGAGGCCTGGGAAT AGGGGAAGCTTGAAACTCTGGGGGAACAATTATAATTCTTGATTCTTTGTGTGATGTGGGTATTGTTTTG AATTTGATTTGGCAACGATTATACAATGTCTTTTTTTCCTATCTGCATTTGGAGTATCAATAAAAGACTG GGGCAAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_177099
• Insert Size: 1107 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC066224, AAH66224
• RefSeq Size: 2534 bp
• RefSeq ORF: 1107 bp
• Locus ID: 320202
• UniProt ID: P57785
• Cytogenetics: 1 H4
• Gene Summary: This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate the mature protein, which plays a role in left-right asymmetry determination of organ systems during development. This protein is also important in self-renewal and differentiation of mouse embryonic stem cells. Mice lacking a functional copy of this gene exhibit defects in axial and left-right patterning. [provided by RefSeq, Aug 2016]