Ercc8 (NM_028042) Mouse Untagged Clone

CAT#: MC201902

Ercc8 (untagged) - Mouse excision repaiross-complementing rodent repair deficiency, complementation group 8 (Ercc8), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ercc8
• Synonyms: 2410022P04Rik; 2810431L23Rik; 4631412O06Rik; B130065P18Rik; Ckn1; Csa
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC037200 sequence for NM_028042
  CAGCGCCCAGGCCTCCAGCCGGTGTGTGGACCCGAGATGTTGAGCTTTTTGTCCGCTCGACAGTCGGGTT TGGAGGACCCTCTTCGCTTGCGTAGAGCGCAGTCCACACGGAGGGTCCTGGGGCTGGAGTTAAACAAAGA CAGGGATGTGGAAAGAATCCATGGCAGTGGGGTTAACACCCTCGACATTGAGCCCGTTGAAGGAAGATAC ATGTTGTCAGGTGGCTCCGATGGCGTGGTTGTACTCTATGACCTTGAGAACGCCAGCAGACAGCCCCATT ACACATGTAAAGCAGTGTGTTCCATTGGCAGAAGCCATCCCGATGTTCACAAATACAGCGTGGAGACTGT TCAGTGGTATCCTCATGACACTGGCATGTTCACATCCAGCTCATTTGATAAAACTCTGAAAGTGTGGGAT ACAAACACATTGCAGGCTGCAGATGTGTTTAATTTTGAGGAAACAGTTTACAGTCATCACATGTCCCCAG CAGCCACCAAGCACTGTCTGGTAGCAGTTGGAACAAGAGGACCCAAGGTACAACTTTGTGACTTAAAGTC TGGATCCTGTTCTCACATTCTACAGGGTCACAGACAGGAAATCTTGGCAGTTTCCTGGTCACCACGCCAT GACTATATCTTGGCAACAGCAAGTGCTGACAGTAGAGTAAAATTATGGGATGTGAGAAGAGCGTCAGGAT GCTTGCTTACTCTTGACCAGCATAATGGGAAAAAGTCACAAGCTGCTGAATCAGCAAACACTGCTCACAA TGGGAAAGTTAACGGCTTATGTTTTACAAGTGACGGCCTTCACCTGCTCACCATTGGCACAGACAACCGA ATGCGCCTCTGGAATAGCTCCAGCGGGGACAACACCCTGGTGAACTATGGGAAGGTGTGTAATGACAGCA GGAAAGGGCTGCAGTTCGCTGTCTCCTGTGGCTGCAGCTCAGAGTTTGTGTTTGTTCCCCACGGCAGCAC CATCGCTGTGTACGCAGTCCACTCGGGAGAGCGGCTCGCCATGCTCAAGGGACATTACAAAAGCGTCGAC TGCTGTGTGTTCCAGCCTAATTTCCAGGAACTTTACAGTGGAAGCAGGGACTGCAATATTCTTGCTTGGG TACCACCTTCCTATGAGCCAGTTCCTGATGATGACGATGAAGCTCCAGCCAAATCCCAGTTAAACCCAGC ATTCGCGGACGCCTGGAGCAGCAGTGACGAGGATGGGTGACTGTCAGCCCTGGCTCCTTGTCCATCAGAC TTCCACGACAGTGTTGTGTGCACTTCAGCACGAGCAGGGTTGTTCTTCCCGTTATTGTTGGTTTTGAGAC AGTGTCTCACGATGTAACCCTGGCTAGCCTGGACTAGCCTGGAACTCTGTATAGAGCAGACTCGGCTCAC ACTGGCAGCTATTCTGCCTCTGCTCCCAGGTGCTGGGGTTAGAGGTGTATACCACGTGGCCCCTCCTGTA CATGTGTGTGATTGTTTGTTTCATTGTTTGTAAACTCTTCAGTCAGTTTTTAACCTGTTGATCCTGAGTT TGGGATATTTCCCTGTGTGCTTTAAAATGAGATCGATGTGTATGATACCCTGAAATATACTTTTCTGTCG GTTAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_028042
• Insert Size: 1194 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC037200, AAH37200
• RefSeq Size: 1629 bp
• RefSeq ORF: 1194 bp
• Locus ID: 71991
• UniProt ID: Q8CFD5
• Cytogenetics: 13 D2.1
• Gene Summary: Involved in transcription-coupled nucleotide excision repair. It is required for the recruitment of XAB2, HMGN1 and TCEA1/TFIIS to a transcription-coupled repair complex which removes RNA polymerase II-blocking lesions from the transcribed strand of active genes. It is the substrate-recognition component of the CSA complex (DCX(ERCC8) complex) which promotes the ubiquitination and subsequent proteasomal degradation of ERCC6 in a UV-dependent manner; ERCC6 degradation is essential for the recovery of RNA synthesis after transcription-coupled repair.[UniProtKB/Swiss-Prot Function]