Dtd1 (NM_025314) Mouse Untagged Clone

CAT#: MC201830

Dtd1 (untagged) - Mouse D-tyrosyl-tRNA deacylase 1 homolog (S. cerevisiae) (Dtd1), nuclear gene encoding mitochondrial protein, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dtd1
• Synonyms: 0610006H08Rik; DTD; DUE-B; Hars2
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC026537 sequence for NM_025314
  GTCGAGGAGCCACCGGCGGAGTCGCAAGGTCTGACCACACAGCAAATTCCAGTCGCTACAGCCGCCGCCA TGAAGGCCGTGGTGCAGCGCGTCACTCGGGCTAGCGTCACAGTTGGAGGAGAGCAGATAAGCGCCATCGG ACGGGGCATCTGTGTCTTGCTGGGTATTTCCATGGAAGACTCACAGAAGGAACTGGAACACATGGTTCGA AAGATTTTAAATCTGCGTGTGTTTGAGGACGAGAGTGGGAAGCACTGGTCCAAGAGCGTGATGGATAAGG AGTACGAGGTGCTGTGTGTCAGCCAGTTCACCCTCCAGTGCGTCCTCAAGGGGAACAAGCCCGACTTCCA CCTGGCCATGCCCACAGAGCAGGCAGAGAGCTTCTACAACAGCTTCCTGGAGCAGCTTCGCAAGAGCTAC AGACCAGAGCTTATCAGAGATGGCAAGTTTGGTGCCTACATGCAGGTGCACATTCAGAACGATGGGCCTG TGACTATAGAGCTGGAGTCCCCTGCTCCTGGAGCTGCTAGCTCTGACCCAAAGCAGCTGTCAAAGCTGGA GAAACAGCAGCAGAGAAAAGAAAAGACCAGAGCCAAAGGCCCTTCTGAGTCAAGCAAAGAACGGAATGCA CCCCGCAAAGAAGACCGCAGCGCCAGCAGCGGGGCAGAGGGTGACGTGTCTTCTGAGCGGGAGCCATAGA TGCGGATGCAGGAGCTCAGCAAATTACCATTGGGCAGAATCGGATCCTGAAAAATCAAGATGCTAAAGCC CCTACACTGCTTTAAGAATTCGGGACTCAAATATGAAAAGAAAATCAGAAATAAGACCCTGGGAACCTGA ATATCCCTGCAGAAAAACACCAAAGGACCATTTTATTTTGTGTATTGCTGTGGTCTGATTGGGGTAGTGG GTGCTGTGGGATGGGAGAGTGCTTGTGGGTTCACACCCATGCCCTCCCCATGGAGAGGGGGCCATTGGTT TTCTTGCTTTCTACCATGTCTTGGCTTGCTTTTGGAACAGGCTGGCCTAGCATCATCTGTCCTCATCTGT GGTGTACTGTGAGTCTGTGATCCATCTCCAGCACATGCCTGCTGTCCACCATTTCCATCATTGAGTCACA AGACAGGGCTGGTGTTAGGCAACCAATAATAACATATTGTATGAGACTTTGCATCTTGTACATTTTCTCT TCTTTTCTAAAAATAAATAATAAAACCATAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_025314
• Insert Size: 630 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC026537, AAH26537
• RefSeq Size: 1239 bp
• RefSeq ORF: 630 bp
• Locus ID: 66044
• UniProt ID: Q9DD18
• Cytogenetics: 2 G1
• Gene Summary: An aminoacyl-tRNA editing enzyme that deacylates mischarged D-aminoacyl-tRNAs. Also deacylates mischarged glycyl-tRNA(Ala), protecting cells against glycine mischarging by AlaRS. Acts via tRNA-based rather than protein-based catalysis; rejects L-amino acids rather than detecting D-amino acids in the active site. By recycling D-aminoacyl-tRNA to D-amino acids and free tRNA molecules, this enzyme counteracts the toxicity associated with the formation of D-aminoacyl-tRNA entities in vivo and helps enforce protein L-homochirality.[UniProtKB/Swiss-Prot Function]