Gtf2f1 (NM_133801) Mouse Untagged Clone

CAT#: MC201828

Gtf2f1 (untagged) - Mouse general transcription factor IIF, polypeptide 1 (Gtf2f1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gtf2f1
• Synonyms: 2810405L04Rik; C76800; RAP74
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC031123 sequence for NM_133801
  AGAGAACGCCGCCAAAAGGTGGCTTTCTCTCCGAAGCTGGTGATCTAGAGTAGTTACAGCGGCTGCGCTC TTCTCGGTGTGGCAGCTCAACTTCCGGGTCAGGCTCTAACGCATGCGCAGAGAGTTAGGTCTCTTTTTCT CGTCTTCCAGCATTCTCCGAGACTTAAGGATTTGGAAATCCAAAGCACAGCATCACACTAACGTGACAAC CAGGAAAGGTGCCTTCCCTTTCCTGGAGCCGAGGGACACAACGAAGTTCATCCCGTCAGGCCGACCGGGA GCAGCTTGAGCAACGGCCATGGCGGCTCTAGGCTCCAGCAGCCAGAATGTCACCGAGTACGTCGTCCGAG TCCCCAAGAACACAGCCAAAAGATACAACATAATGGCTTTTAATGCAGCCGATAAAGTCAACTTTGCTAC CTGGAACCAGGCACGGCTGGAGCGAGATCTGAGCAACAAGAAGATTTACCAGGAAGAAGAGATGCCAGAG TCCGGTGCAGGCAGTGAATTCAACCGCAAGCTTAGGGAGGAGGCGCGCCGGAAGAAGTATGGCATTGTCC TAAAGGAGTTTCGGCCTGAGGATCAGCCTTGGCTACTCCGTGTCAATGGCAAATCAGGACGGAAGTTCAA AGGCATTAAGAAAGGCGGGGTAACAGAGAACACAGCCTACTACATCTTCACACAGTGTGCTGATGGTGCC TTTGAGGCTTTCCCTGTACAGAACTGGTACAATTTCACACCACTGGCCCGACACCGCACACTCACTGCTG AGGAAGCCGAGGAGGAATGGGAGAGGAGGAACAAGGTCCTAAACCACTTCAGCATCATGCAGCAGCGGCG GCTCAAGGACCAGGACCAGGATGAAGATGAGGAGGAGAAGGAGAAGCGCAGTCGCAAGAAGCCTAGTGAG CTGCGCATTCATGACCTGGAGGATGACCTGGAAATGTCCTCCGATGCCAGCGATGCCAGTGGTGAAGAGG GCAGCAGAACCTCCAAGGCTAAGAAAAAGGCCCCTGTGACCAAGGCAGGCAGGAAGAAGAAAAAGAAGAA GGGCTCAGATGATGAGGCTTTTGAGGACAGCGATGATGGGGACTTCGAGGGCCAGGAGGTAGACTACATG TCTGATGGCTCCAGCAGCTCTCCAGATGAGACAGAAGGCAAGCCCAAAGTGCCCCAGCAGGAGGATGGCC CAAAGGGGGTGGATGAGCAGAGCGAGAGCAGTGAGGAGAGTGAGGAGGAGAAGCCCCCCGAGGAGGACAA GGAGGAGGAAGAAGAGAAGAAGGCCCCTACCCCACAAGAGAAGAAGCGCAGGAAAGACAGCAGTGATGAC TCAGACAGCTCAGAAGAGAGCGACATTGACAGTGAGACCTCCTCTGCACTCTTCATGGCGAAGAAGAAGA CACCCCCAAAGAGGGAGCGGAAGCCATCGGGGGGGAGTTCAAAGGGCACCAGTCGGCCAGGGACTCCCAG TGCAGAAGCAGCAAGCACCTCTTCTACCCTGCGGGCTGCAGCCAGCAAGCTGGAGCAGGGGAAGCGGACG AGTGAGACTCCAGCAGCCAAGCGTCTTCGGATGGACACGGGTCCCCAGAGCCTGTCTGGGAAGTCCACAC CCAGCAGCGGTGATGTCCAGGTGACAGAGGACGCTGTGCGCCGCTACCTGACCCGCAAGCCCATGACCAC AAAGGACCTGCTGAAGAAGTTCCAGACCAAGAAGACAGGGCTGAGCAGCGAGCAGACAGTAAATGTGCTG GCGCAGATCCTCAAGCGCCTCAACCCTGAGCGCAAGATGATTGGTGATAAGATGCACTTCTCCCTCAAAG AGTGATGCCAATAAAGAGAGCAGGCCCAGAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_133801
• Insert Size: 1527 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC031123, AAH31123
• RefSeq Size: 1865 bp
• RefSeq ORF: 1527 bp
• Locus ID: 98053
• UniProt ID: Q3THK3
• Cytogenetics: 17 D
• Gene Summary: TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation (By similarity).[UniProtKB/Swiss-Prot Function]