Dis3l2 (NM_153530) Mouse Untagged Clone

CAT#: MC201819

Dis3l2 (untagged) - Mouse DIS3 mitotic control homolog (S. cerevisiae)-like 2 (Dis3l2), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dis3l2
• Synonyms: 4930429A22Rik; 8030493P09Rik
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC036177 sequence for NM_153530
  GCGGCGCCGCCGGCCTCCCGGGAGCGACGCTCGTGACAACTGAGCTGCTGAAGGCAGGAGGAACTCTGAG CTGAATAGTAGTGGGTCCCTGAATCTGGAGAGAAGACGCCACCTTGGAACCAGTAATGAACCATCCTGAC TACAAGCTGAACCTTCGGTCTCCGGGGACCCCCAGAGGTGTGTCCTCTGTGGTTGGCCCGAGTGCTGTTG GTGCTTCGCCAGGTGACAAAAAGTCAAAGAACAAGTCCATGCGAGGGAAGAAAAAGAGCATATTTGAAAC CTACATGTCCAAGGAGGATGTTTCAGAAGGCTTGAAGAGAGGAACACTTATCCAGGGTGTATTGAGAATC AACCCAAAGAAGTTTCATGAAGCCTTCATTCCTTCTCCGGATGGTGATCGGGACATTTTTATTGATGGAG TTGTTGCTCGTAATAGAGCCTTAAATGGGGACCTTGTGGTTGTAAAACTGCTTCCTGAGGATCAGTGGAA GGCAGTTAAACCAGAGAGCAATGACAAAGAAATAGAAGCTACTTATGAAGCTGACATCCCTGAAGAGGGC TGTGGACATCACCCCCTGCAGCAGTCCCGGAAAGGCTGGAGTGGTCCTGATGTCATTATAGAGGCTCAGT TTGATGACAGCGACTCAGAAGATAGACATGGCAACACCAGTGGCCTGGTTGATGGTGTTAAGAAATTGTC AATCTCTACTCCTGACAGAGGAAAAGAAGATTCTAGTACTCCAGTTATGAAAGATGAGAACACCCCCATA CCACAGGACACAAGAGGCTTATCAGAGAAGTCACTTCAGAAATCAGCAAAGGTGGTTTACATCTTGGAGA AAAAGCATTCTCGAGCAGCAACTGGCATCCTGAAACTCTTGGCTGATAAGAACAGTGACCTGTTTAAGAA ATACGCCCTGTTTTCTCCTTCAGACCACCGAGTACCTAGAATTTACGTACCTCTCAAGGACTGTCCCCAG GACTTCATGACCCGACCTAAAGACTTTGCCAACACGCTGTTCATCTGCCGCATCATAGACTGGAAGGAGG ACTGTAATTTTGCCCTGGGGCAACTGGCTAAGAGTCTTGGGCAGGCTGGTGAAATCGAGCCTGAAACAGA AGGGATACTGACAGAATATGGTGTGGACTTCTCTGATTTCTCTTCAGAAGTTCTTGAATGTCTCCCTCAA AGCCTGCCCTGGACAATCCCACCTGATGAGGTGGGCAAGAGAAGAGACCTAAGGAAAGACTGTATCTTCA CCATTGATCCATCAACTGCTCGCGACCTTGATGATGCCCTCGCCTGCAGGCGGCTCACTGATGGCACCTT CGAAGTGGGCGTCCACATCGCCGATGTGAGTTACTTTGTTCCTGAGGGATCCTCTTTGGATAAAGTAGCT GCTGAGAGAGCCACAAGTGTCTACTTGGTCCAGAAGGTGGTCCCCATGCTTCCCAGGCTTCTGTGTGAGG AACTCTGCAGCCTCAACCCCATGACTGACAAGCTGACCTTCTCTGTGATCTGGAAGCTGACCCCTGAAGG CAAGATCCTTGAAGAGTGGTTTGGCCGCACTATCATCCGTTCTTGCACCAAACTGAGCTACGACCATGCC CAGAGCATGATCGAAAATCCAACTGAGAAGATCCCTGAGGAAGAGCTTCCCCCAATTTCTCCAGAGCACA GCGTCGAGGAGGTGCACCAGGCAGTCCTGAACCTGCACAGCATTGCAAAGCAACTCCGCCGCCAGCGCTT TGTAGATGGCGCACTCCGTTTAGATCAGCTGAAGCTTGCTTTTACTCTGGACCATGAGACTGGATTGCCT CAAGGATGTCACATCTATGAGTACCGAGACAGCAACAAGCTTGTAGAGGAGTTCATGCTCCTGGCCAACA TGGCGGTGGCCCACAAGATCTTCCGCACCTTCCCTGAGCAGGCCCTGCTGCGCCGGCATCCCCCACCACA GACGAAGATGCTCAGTGACCTGGTGGAGTTCTGTGACCAGATGGGGCTGCCCATGGATGTCAGCTCTGCA GGGGCCCTAAATAAAAGCCTGACTAAGACATTTGGAGATGACAAGTACTCTCTGGCCCGGAAGGAGGTGC TCACCAACATGTACTCCCGGCCCATGCAGATGGCACTGTACTTCTGCTCTGGGATGCTGCAGGACCAGGA GCAGTTCCGGCATTATGCTCTCAACGTTCCCCTCTACACACACTTCACCTCTCCCATCCGCCGCTTTGCT GACGTCATAGTGCACCGCCTCCTGGCTGCTGCTCTGGGCTACAGTGAACAGCCAGATGTGGAGCCTGATA CCCTACAGAAGCAAGCTGACCACTGCAATGACCGTCGCATGGCTTCCAAACGTGTGCAGGAGCTCAGCAT CGGCCTCTTCTTCGCAGTTCTAGTAAAGGAGAGTGGCCCCCTGGAGTCCGAAGCCATGGTGATGGGTGTC CTGAACCAAGCTTTCGACGTGCTGGTGCTGCGCTTTGGGGTGCAGAAGCGCATCTACTGCAATGCACTGG CCCTGCGATCCTACAGCTTCCAGAAGGTGGGGAAGAAGCCAGAGCTCACTCTTGTTTGGGAGCCTGATGA CCTTGAAGAGGAGCCAACACAGCAGGTCATCACCATCTTCAGCCTGGTGGATGTGGTCCTGCAGGCAGAG GCCACAGCCCTCAAGTACAGTGCTATCCTGAAGCGACCAGGCCTGGAGAAGGCGTCTGATGAGGAGCCTG AGGACTGAATGCTAGCCCAAGCCAGGCCTGTGCCTGCCCTACCCTGCTGGCTTTTAGGAATAGGACCTTT TGACACCAAAGGGGATTTTTAATTTGGTTTTTAACAACTCAGGGGTTTGTTTTTATTTTTATTTTTCCTT TTATTTTACTTTTGCAGCTCAGTTTTTAAATGAACTGGAAGGTTAGGGGTCAGGGCAGGGGATGCTGAGG CCTGGCCTGTGCTTCCCTGAGCAGAGAGGATCCCAGTCCTCCTGGGCAGGCAGCCCCGCTTCTACCAGGC GACCCACTGCCCTTCCCTGCCCAGGAAATGGGGGGTTTCAGCAAATCAGTGTCATGGAATAAAATCAAGT GTGAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_153530
• Insert Size: 2613 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC036177, AAH36177
• RefSeq Size: 3116 bp
• RefSeq ORF: 2613 bp
• Locus ID: 208718
• UniProt ID: Q8CI75
• Cytogenetics: 1 C5
• Gene Summary: 3'-5'-exoribonuclease that specifically recognizes RNAs polyuridylated at their 3' end and mediates their degradation. Component of an exosome-independent RNA degradation pathway that mediates degradation of both mRNAs and miRNAs that have been polyuridylated by a terminal uridylyltransferase, such as ZCCHC11/TUT4. Mediates degradation of cytoplasmic mRNAs that have been deadenylated and subsequently uridylated at their 3'. Mediates degradation of uridylated pre-let-7 miRNAs, contributing to the maintenance of embryonic stem (ES) cells. Essential for correct mitosis, and negatively regulates cell proliferation.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region, compared to variant 1. The resulting isoform (2) lacks an internal segment, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.