Chtf18 (NM_145409) Mouse Untagged Clone

CAT#: MC201779

Chtf18 (untagged) - Mouse CTF18, chromosome transmission fidelity factor 18 homolog (S. cerevisiae) (Chtf18), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Chtf18
• Synonyms: 6030457M03Rik; Chl12; CTF18
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC024142 sequence for NM_145409
  GCGCGGTGGCGGCGGCGCGGGAAGTTCTGAGGGACTCTGAGCTGTGGGAGTTGTGGTCTGGCTCCATGGA AGACTACGAGGAAGATCTGTATGGTGTGGAGGATGATTTCCAAAACCAGTTCGCAGCTGAGCTCGAAGTG CTGGCTGAGCTGGAAGGAACACGGGACCAGGCTCCTCCTGGGACCCTCCAAACTCCAGCGAGCCGCCCTC CGCTAACCTTCGAAGAGGCCATCGCTGGTGGGGACACTGTCCCGCGTCCCTGCCCTGCAGGGTCGCCAGG AAATGTCAACCGCAATACTAGAAAGAATGTTCGTAGGGACCAGCCCGCGCCCTCCAGCCCCATGGTCAAA CGACCCAGGTTGGACGTGGTGAAGAAGCTGAACTTTGAGCCAGATATGGAGGAGCTCTTATATCCAGATT CCCCTCCTGGGGACATTACCCCTCCACCGAGTCCAGAGGTCTTCCCTGAGATGTTGGATGCTGGGTATTC AGATGCTAATGCTGACAAAGACCTCATGCAGACATTGCCAAGTCCCCGAAATCGCAATCCTGTCCTGAGA CGGCCTCCTATCTTAGAAGATTATATCAATGTGACATCCACAAGTGGAGAACGGGCCTTTTTGGTGCTTC GGGCTGACCTCATAGGCCCTGGGGTGCAGAACCCTCTTCTAGATGTCCACTGGCGAGGCTGTGGCCAACT GGACCTGCTGGGTGTCCCCTTCGCCTCCCTGAAGGAGCAAGTTGATAGCAAGAGACGGCAACAACTGCTG GAGGATGCCCAACAGCTTTCAGACACTCTACACAGCCTCAGGTCTGAAGGAGAGGAGGCTGTGCTTGAAG GGCCCCCTGCGGAGGAGCCAGCCCCTGGCCAGAACACCGCTCAGCACTGCCTCTGGGTGGATGAGTTTGC ACCTCAGCATTACACGGAACTGCTCAGTGACGATTTTACCAACCGCTGCCTACTCAAGTGGCTGAAGCTA TGGGACCTTGTGGTGTTTGGCCGTGAGAGACCTGCCCGGAAGCCCAGACCTGGTGTAGAGACCACCCGGG TTGGGAAGGAGGCTACAGCCCCTGGCAAGTGGAAAAGCCATGAGCAGGCACTGGAGGAGATGCTAGAAGC TGAACTGGACCCTAGCCAGAGGCCTCGGCAGAAGGTGGCACTGTTGTGTGGCCCTCCAGGACTAGGCAAG ACCACGCTGGCACATGTGGTCGCAAGGCATGCAGGGTATTGCGTGGTAGAGATGAATGCAAGTGATGACC GTAGCCCGGAGGCCTTCCGTACACGCATTGAAGCAGCTACGCAAATGGAGTCGGTTCTGGGTGTGGGTGG GAGGCCCAACTGCCTGGTTATTGATGAGATCGACGGGGCCCCCACGGCGGCCATCAACGTTCTTTTGGGT ATCCTGAATCGCAAGGGTCCGCAGGAGGCCGACCAAGGAGGCACAGCTGTGGCCGCAGGGGGGCGGCGGC GCAGGGCCGAGGGGGGTCTCCTAACCAGGCCTATCATCTGCATCTGCAATGATCAGTTCACACCTTCTCT GAGGCAGCTGAAGCAGCAGGCTCTTCTGCTCCACGTCCCCCCAACTCTGCCCTCCAGGCTGGTGCAGCGG CTCCAGGAGATCTCCCTGCAGCATGGCATGCGCTCTGACCCGGGTGCACTGGTTGCCCTCTGTGAGAAGA CCGACAATGACATCCGTGCCTGCATCAACACCCTGCAGTTTCTGTATGGGAGGGGCCGGCGGGAGCTGAG TGTGAAGGCTGTGCAGACCACTCATGTCGGCCTGAAGGACCAGCGCAAGGGGCTATTCTCTGTGTGGCAA GAGGTCTTCCAGTTGCCCAGGACTCAAAGGAGAATCGTGGGCCAGGACCTGATGCTGCCAGCCCACGCTC TCCTGCTCAGCAATGGTGACAAGGGCTCTCTGACCCTGGCCTCCCAGCGCTTTTACCACATCCTGCGTGT GACCACCTCTGCGGGCGAGCATGAGAAGGTTGTCCAGGGCCTGTTTGACAACTTTCTACGCCTTCGGCTT CGGGACTCCAGCTTAAGCACCGTGTGCTGTGCCCTCGACTGGCTGGCCTTTGATGACCTGCTGGAGCAGG CTGCCCACCGTGGCCAGAGCTTCCAGTTGCTGTGCTACTTGCCCTTCGTGCCTGCGGCCTTCCACGTGCT CTTTGCCTCCAGCCATGTGCCACGAATCACTTTCCCCAGCAGCCAGCAGGAGGCCCAGACTCGGATGAGC CAGACAAGGAACCACATACAGACACTGGTGTCAGGTATGGCACCGACCACGCGTAGCCGGGCCACACCAC AGGCCCTTGTTCTAGATACTCTCTGCCTGCTCCTGGATGTCCTCGCACCCAAGCTGCGCCCCGTGAGCAC ACAGCTGTACAGTGCCCATGAGAAGCAGCAGTTGTCCTGCCTTGTGGGTACCATGCTTGCCTACAGTCTC ACCTACCACCAGGAGCGCACACCTGATGGGCAGTACCTCTACAAGCTGGAGCCGAATGTGGAAGAGGTCT GCCGCTTCCCTGAACTACCTGCCCGAAAGCCCCTCACCTACCAGGCTAAGCAGCTTATTGCTCGGGAGAT TGAAATGGAGAAGATGCGCAGGGCAGAGGCTTTGGCCTGGGCTCGAAGTGGCCCCCAGGTGGACCAGGGC TCCTCAGGGCCTGCAAGTCTGTGGACAGACAGTGGGGAAAAGGAGACAAGGCAGCCTGCCCCACGTAACC ATGAGCAGCGCCTAGAACACATCATGAAGAGAGCAACCGTGCAGGAGCAGCCTGAGAGGGACTTCTTTGG ACGTGTGGTCATCAGGAAAGTGGCAGTCCCGAGCAGAGAGGTTGAGGCCCCCCAGAAGGACGCGGACGAG TGGCGCATGGGTGTGGCAGTGGGCAGGAGTGAGGTGTGGTTCCGGTTCAACGAGGGCGTCTCAAATGCTG TTCGGCGCAGCCTGTACATCAGGGACCTGCTGTAGCCCCACTGCCCCAGGACATCAGATCGCACTGGCTC CCACAGGGAATGGACTAAAGCAGAGACACCCGGAGAAGTCTATTTATAAAGTCACACCTTACAGAGAGTG TGGGTCAGGCTCAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_145409
• Insert Size: 2910 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC024142, AAH24142
• RefSeq Size: 3110 bp
• RefSeq ORF: 2910 bp
• Locus ID: 214901
• UniProt ID: Q8BIW9
• Cytogenetics: 17 A3.3
• Gene Summary: Chromosome cohesion factor involved in sister chromatid cohesion and fidelity of chromosome transmission. Component of one of the cell nuclear antigen loader complexes, CTF18-replication factor C (CTF18-RFC), which consists of CTF18, CTF8, DCC1, RFC2, RFC3, RFC4 and RFC5. The CTF18-RFC complex binds to single-stranded and primed DNAs and has weak ATPase activity that is stimulated by the presence of primed DNA, replication protein A (RPA) and by proliferating cell nuclear antigen (PCNA). The CTF18-RFC complex catalyzes the ATP-dependent loading of PCNA onto primed and gapped DNA. It also interacts with and stimulates DNA polymerase POLH (By similarity).[UniProtKB/Swiss-Prot Function]