Micu2 (NM_028643) Mouse Untagged Clone

CAT#: MC201768

Micu2 (untagged) - Mouse EF hand domain family A1 (Efha1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Micu2
• Synonyms: 1110008L20Rik; 4833427E09Rik; AU041871; Efha1; Smhs2
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC031172 sequence for NM_028643
  CGGACGCGTGGGCGCTTCCGCAAAGATGGCGGCGGCTGCGGGAAGAAGCGCTTGGCTGGCGGCCTGGGGC GGAAGGCTGCGGCGCGGGCTCGCGGCCGGCCGACGAGCTGTGCCGACTCGCGGCCCTCTCGCAGCGGCTG TGGCCGGGGTAGCCCTGGCAGGCGCAGGAGCGGCATGGCATCACGGCCGCGTGAAAGCAGCCGCGCGCGA GGGCTCCCGAACGGTGTCGGCGCAGAAAAATTATTTGGGACCAATAGAGAAACTTTCTCTCCGCAAACAG CGGTTCATGCAGTTTTCATCCCTGGAGCACGACGGAGAGTATTACATGACACCCCGAGACTTCCTCTTCT CAGTCATGTTTGAGCAAGTGGAGCGTAAAACACTGGTCAAGAAGCTGGCAAAAAAGGATATCGAGGATGT ACTGTCAGGAATCCAAACAGCACGCTGTGGATCAACATTTTTTAGAGACCTGGGTGATAAAGGGGTAATT TCATATACCGAGTATCTTTTCTTGCTTACAATCCTCACAAAACCTCACTCTGGGTTCCATGTTGCTTTTA AAATGCTGGATGTGGATGGGAATGAGATGATCGAGAGGAAGGAGTTTGTTAAGCTGCAGAAGATCATAAG TAAACAAGATGGCTTCAAGACAGTGAAGACTAATGAGACAGAATACCAGGACCCAACAGTGAAAGAGCCT GGCGTTAACACAACCCTTCAAGTGCGTTTCTTTGGGAAAAGAGGAGAAAAAAAACTCCATTATAAAGAAT TTCGAAGATTTATGGAAAATTTACAAACAGAAGTTCAAGAAATGGAATTCCTTCAGTTCTCTAAAGGCCT GAATTTCATGAGAAAAGAAGACTTTGCAGAATGGCTACTTTTCTTCACTAACACTGAAAATAAAGATATT TACTGGAGAAACGTGAGAGAAAAGTTGTCAGTAGGAGAGAGCATTAGTTTGGATGAGTTCAAGTCCTTTT GCCATTTTACAACGCATTTGGAAGACTTTGCTATCGCCATGCAAATGTTTAGTTTAGCTCATCGCCCTGT GCGGCTGGCAGAGTTCAAGAGAGCTGTGAAGGTAGCGACTGGACAGGAGCTCTCGGACAATCTTCTGGAC ACCGTCTTCAAGATCTTTGACCTGGACGGGGACGAGTGCCTCAGCCACGGCGAGTTCCTTGGGGTACTGA AAAACAGGATGCACCGAGGCTTATGGGTGTCACAGCAGCAGAGTGTGCAAGAATACTGGAAGTGTGTGAA GAAGGAAAGCATCAAGGGAGTAAAGGAAGCCTGGAGACAACAAGCCGGCAAGGGCCCCTTCTAAGGGGAA GCTGATGGAGCTCCAGGAGCCAGAAAGTGGTTTTTCCAAGAGCTGACTGTTTCTATGTCTTCATTGAGTT TCTTTTGAGTGTGAAGATGTGAAGACTCACTTTCTGATTTGGGGGATTTGGGAGGGATGACGGGAGGTGA ATAAGTGTGTGCCACAGTGGGAAGTCAGAGGCCAGCCTGTAGGAGCTGGTTCTCTCTGTCTACCATGGGA GTCCCAGAGATTGAACTCAGGTCTCGAGCTTTGACATCAAGTGCCTTTACCTGATGAGCCATCTCACCAG CTCCGACTCCATCATTTCTTGGTGAATTTTCATTACACAAAGTATTCCTTTATGAAAATACACACTGCTC TATTAAAGACTTGTGTACAAAGTGTGCTTTCTGGAGGGCTAAACAAGTCAGGGAAGGAACTTCATTCCCA AGACAGGTACTGATCCCAGAAGCCGTGATAGCTTAGGGAATACTTGCAGTTAAAGGCTTGCTCCAGTGTT CTTTGCTCCCTGCCTTGAAATAGCACATAATGGCAGTGTCCGGTCTGGCAGCAGGATGAAGTATTTACAT TGATACAAAAAAATTTTTTCTTTTTTAAATGTTCCATGCTTTTGAAATATAAATAGAGTGTCCTCTCCCA GACACTCCCAGGGGAGCTCAGCCAGCAAAGGACACAGCTGTGTACCATGCTTTCCTAACAAGCAGCACAC AGAGTTCCATCTAAAACCACTCATACACACACAGATCACACACAGATCACACACATACAGTGATCATAAT AGCAAGTTTTCTTAAGCTACGGGATCTTGGGTTGTGGGAAATATGGCACTATAAAGTAGCTTGCAATAAA ATAAATAGGAAATAATGTTATGTGGAGATGGACATGTGTATGTATACAGGTGGACATACATGTGTGTGTG GAAGCCCCTGCTCCTGTAAATACCCCCATGTTTCTGAAGTGATCCCAATAAACCCATGAGCTCACCAAGC TAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_028643
• Insert Size: 1299 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC031172, AAH31172
• RefSeq Size: 2333 bp
• RefSeq ORF: 1299 bp
• Locus ID: 68514
• UniProt ID: Q8CD10
• Cytogenetics: 14 C3
• Gene Summary: Key regulator of mitochondrial calcium uniporter (MCU) required to limit calcium uptake by MCU when cytoplasmic calcium is low (PubMed:23409044, PubMed:24560927). MICU1 and MICU2 form a disulfide-linked heterodimer that stimulate and inhibit MCU activity, depending on the concentration of calcium (PubMed:24560927). MICU2 acts as a gatekeeper of MCU that senses calcium level via its EF-hand domains: prevents channel opening at resting Ca(2+), avoiding energy dissipation and cell-death triggering (PubMed:24560927).[UniProtKB/Swiss-Prot Function]