Slc29a4 (NM_146257) Mouse Untagged Clone

CAT#: MC201750

Slc29a4 (untagged) - Mouse solute carrier family 29 (nucleoside transporters), member 4 (Slc29a4), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Slc29a4
• Synonyms: ENT4; mPMAT
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC025599 sequence for NM_146257
  CCACGCGTCCGCCGGGACACCGAGCAGGACGCCCGCCGCGCCCGGTCCCAAGCCTCGAGAAGCGGGGCAC AGCGCAAAGGGTCCGGGCGCGGGCGGCAGGCAGGCAGCGAGGATCCCAGCCTGGGGCCGCCCGAGTGTGA ACTGCCATGGGCTCTATCGGAAGCCAGCGCCTCAAGGAGCCCTGCGTGGCAGCCACATCTGACCAGAGTG TGGTGACAAGCTTTAGCTTTGACAACTTCCAGCTGGAGACGACAGCAGAGGGGGCTCAGGATCCAGGCAT CAGAGTCAGGGGTGTCCCCACTTTCACAGACTCTGCGGTAGAGGAGCCTGTGCCAGATGACCGCTACCAT GCCATCTACTTTGCCATGCTGCTGGCTGGCGTGGGCTTCCTACTGCCATACAACAGCTTTATCACCGATG TCGACTATCTTCACCACAAGTACCCAGGGACCTCCATCGTATTTGACATGAGCCTCACCTATATCCTGGT GGCATTGGCGGCTGTGCTCCTAAACAACGTTGTGGTGGAGAGGTTGAACCTGCACACCAGGATCACCACA GGCTACCTCCTCGCCTTGGGTCCCTTGCTCTTTATCAGCATATGCGATGTGTGGCTGCAGCTCTTCTCTC ACGACCAGGCTTACGCCATCAACCTGGCCGCCGTGGGCACTGTGGCCTTTGGTTGCACAGTGCAGCAATC TAGCTTCTATGGCTACACGGGATTACTGCCCAAGAGGTACACGCAGGGTGTGATGACTGGAGAGAGCACG GCAGGGGTGATGATCTCTCTGAGTCGAATCCTCACTAAGCTGCTGCTCCCGGACGAGCGGGCCAGCACCA TCATCTTCTTCCTGGTGTCTGCCGGCTTGGAGCTCCTGTGCTTCCTGCTGCATCTGCTGGTGCGGCGCAG CCGTTTTGTGCTCTACTATACCACTCGGCCCCGTGACAGCCGCCCCGTCCAGGCAGGCTACCGAGTGCAC CACGATGTCGCATCCGGAGACATCCACTTTGAGCACCAAACTCCAGCCCTATCCAGCAGCAGGTCCCCGA AGGAGAGCCCGGCCCATGAGGTGACGCACAGCAACAGTGGGGTCTACATGCGTTTTGATGTGCCCCGGCC ACGAGTCAAACGAAGCTGGCCCACCTTCAGAGCCCTGCTGTTGCACCGATACGTCGTGGCGCGGGTCATC TGGGCCGACATGCTGTCCATTGCGGTAACCTACTTCATTACACTGTGCCTGTTTCCGGGCCTGGAGTCCG AGATCCGCCACTGCGTGCTGGGGGAGTGGCTGCCCATCCTGGTGATGGCCGTGTTCAACCTGTCAGACTT TGTGGGCAAGATCCTGGCAGCCCTGCCCGTGGAGTGGCGGGGCACTCACCTGCTCGCCTGCTCTTGCCTG CGTGTGGTCTTCATCCCCCTCTTCATCCTGTGTGTGTACCCCAGTGGCATGCCTGCCCTCCGCCACCCTG CCTGGCCCTGTGTCTTCTCGCTGCTAATGGGCATCAGCAATGGCTACTTTGGCAGTGTGCCCATGATCCT GGCAGCTGGAAAAGTGAGCCCCAAGCAGCGGGAACTGGCAGGAAACACCATGACTGTGTCCTACATGTCG GGGCTGACCCTGGGCTCTGCTGTGGCCTACTGCACCTACAGCCTCACCCGAGATGCCCACGGCAGCTGCT TTCAAACAGCCACCGCCGCCGCCGCCAACGACTCCATCCCTGTCGGTCCCTGAGCCGGGCCCACCCAATC CTGCCCCCTGCCCAGGAAGAGCTGCCTGGGCCAGTTACCCCAAGGCCTGAGGACCCTCCCACCCCTGCCT CAGTGCCTGCGGGGTCCTGGCCACCTCCTAAGCCACTAACGCCACCTTGCCACCTTGTCCCAGTTGTCCA AAACAGCTGGAACTTGTCTTCACCGCCCAGGGCACCGCCTCCCCGTGTCCTGTGTTTGGTCAGACACCTG CCCTTCACCCTCTGTGTCCAGAGGCCCAGCATCTGTCACACATCAAGGGGGCGTTTATTCAGTCTTAGAG CCACTCGGAATGGCTGGCCACAGTCTGAGCTGACTCCCTTCAGTGTGACCCCACTGTGTGGTTCCATGGT CTTCGATGTGGTTTTCATTTACTGGTCAGGGGGCACCTCCCTGTATTAGAAATCTTGGAGGAAAGTCCCC AACTCCGTTCTGTCCCTTTCTTGTTCTTAGACACTTCTTCACAGCCCTACCCCTGCACCCGCACACATAA AGCGTCAAGTCTGGAAAACTCATTCCTCATACACAGGCCTCCTGTAGTCATAGCAACAGAGATGACCTAG GTGCACCCACCTTAGGTGGAGGACTTCCCCATGCACACCACCACCCCTCTTAGAGCACAGGCTAAGGGCC ACGGCTAACAGGGACGTCCCATTTCTTTCCTGTTCCATCCAACTTACCCATCATGCAGGGACAGCTGAGG TCCACTCGTGACCTAGGCCGCCCCACTCCCATCCACCGAGAGTCAAGTGATGACACGACACACTTGAAAC CCTGGCAAGTGGCTGCAAGGTGAGGATTGGACTCGTGGGTGCCAGGATCGGCTGGAGGACCTCCTGCCAA AGAGAGCTTTTGCTGAGCCGGGACACTTGCCTCTCCACACAGTGGCCCCAGCCGTGACCACCCTGTACCA GTCCCTGCTGTGTCCTGTCTACCTGTGTGTTCTCTGACTACCGCACTGGCCATTAAATATGGCAGAGACT GCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_146257
• Insert Size: 1587 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC025599, AAH25599
• RefSeq Size: 2886 bp
• RefSeq ORF: 1587 bp
• Locus ID: 243328
• UniProt ID: Q8R139
• Cytogenetics: 5 G2
• Gene Summary: Functions as a polyspecific organic cation transporter, efficiently transporting many organic cations such as monoamine neurotransmitters 1-methyl-4-phenylpyridinium and biogenic amines including serotonin, dopamine, norepinephrine and epinephrine. May play a role in regulating central nervous system homeostasis of monoamine neurotransmitters. May be involved in luminal transport of organic cations in the kidney and seems to use luminal proton gradient to drive organic cation reabsorptprev reabsorption. Does not seem to transport nucleoside and nucleoside analogs such as uridine, cytidine, thymidine, adenosine, inosine, guanosine, and azidothymidine. In (PubMed:16873718) adenosine is efficiently transported but in a fashion highly sensitive to extracellular pH, with maximal activity in the pH range 5.5 to 6.5. Glu-206 is essential for the cation selectivity and may function as the charge sensor for cationic substrates. Transport is chloride and sodium-independent but appears to be sensitive to changes in membrane potential. Weakly inhibited by the classical inhibitors of equilibrative nucleoside transport, dipyridamole, dilazep, and nitrobenzylthioinosine. May play a role in the regulation of extracellular adenosine concentrations in cardiac tissues, in particular during ischemia (By similarity).[UniProtKB/Swiss-Prot Function]