Phlda3 (NM_013750) Mouse Untagged Clone

CAT#: MC201656

Phlda3 (untagged) - Mouse pleckstrin homology-like domain, family A, member 3 (Phlda3), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Phlda3
• Synonyms: Tih1
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC023408 sequence for NM_013750
  GGAGCGGGCGTGCGGGCGGCGAGGCGCAGGGCACCGGGTAGAGGCTCGGGGAGGTCGGAGCGGCGCGCAG GGTTCCAAGCCGGCGGCGAGAGAGTAGCATCGCACGAGCCTCCCGGTACCCGCTGTCTGCGAGGCGGCCC GGGCCGCAGGCTCGACCGCACAGGGAGCAAGGCCAACCAGCTCCAGGGCGCGAGAAGCCGGCGCTGTAGA GCCGGGACAGCCGGGATCGCCGCAGCCTCCAGAGCTGTGGGAACCTCGGGGTGCCCATGACGGCGGCGGC GACCGTGCTGAAGGAGGGCGTGCTGGAGAAGCGCAGCGGCGGGCTGCTGCAGCTGTGGAAGCGGAAGCGC TGCGTGCTCACCGAGCGCGGGCTGCAGCTCTTCGAGGCCAAGGGCACGGGCGGCCGGCCCAAGGAGCTCA GCTTCGCCCGCATCAAAGCCGTGGAGTGCGTAGAGAGCACCGGGCGCCACATCTACTTCACGCTAGTGAC CGAAGGCGGGGGCGAGATCGACTTCCGCTGCCCCCTCGAAGACCCTGGCTGGAACGCTCAGATCACCCTG GGCCTGGTCAAGTTCAAGAATCAACAGGCCATCCAGACTGTGCGGGCCCGGCAGAGTCTTGGGACTGGGA CCCTTGTGTCCTAAACCATGAGGCGTATCATTTTATCGACATGCCCCCCCTCTCCCATCTCCGTGCCCAG AAGACATGTCAGCTTCTCTGTCCACTTTGGTTGGTTGGAGTCAGGTTACATGTGATGAGCCAGAAGCTAT CAACATGAGGAAGATAGGCCTATCATCATGGAAGGAGCCAACCAGCCAGAGACGGAGAGCAGAAGCCAGT AGGGGCTGAGCATGAAGATTCAGTTCCTGGACACTAAGACTGTTGTTATATCCAGCTCAGACCTGCGAGC CACAGGCCTGGCCTCCAGTATCATGATGGAGTACTTGGGCCTTACCTGGTCCAGCCCTTGGTTCTGGGTT CTGCAGACTGCTGTTTGACCTCTGGCTTTGAGACATGCCCAAAGAAGGGCTGGCTGTTCTTCATGGCGTG CTAAGCCAGTGCCTCAGAACTCAGGAGGCCAGCCTGGGGTCCAGAAGATGACCACCTTACCTTAGGACAG CCACTGGACTCAGCTTGTGGAGGGGGGTCTGCTGGGCTGGAGTCCTGTGCCTGGGGGGGGTCTCCTGCTG CTTAGGTCCTTTTGGGACCCCCCCGCCCATCTGTACCCTTTCTTTGCACACTTCTTCCCTCACCCCTGTT GCCCTCTCCTCACTTCGGTGTTGGGCCTGGAGGGGGTGGGGGTGGGGTAAGGGTTGGCCCTTTACCATTT CATGCTTATTCCAGAATGAAGAGGCCCAGTTCGTGGGCAGTAACCAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_013750
• Insert Size: 378 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC023408, AAH23408
• RefSeq Size: 1390 bp
• RefSeq ORF: 378 bp
• Locus ID: 27280
• UniProt ID: Q9WV95
• Cytogenetics: 1 59.26 cM
• Gene Summary: p53/TP53-regulated repressor of Akt/AKT1 signaling. Represses AKT1 by preventing AKT1-binding to membrane lipids, thereby inhibiting AKT1 translocation to the cellular membrane and activation. Contributes to p53/TP53-dependent apoptosis by repressing AKT1 activity. Its direct transcription regulation by p53/TP53 may explain how p53/TP53 can negatively regulate AKT1. May act as a tumor suppressor (By similarity).[UniProtKB/Swiss-Prot Function]