Pdcd6ip (NM_011052) Mouse Untagged Clone

CAT#: MC201554

Pdcd6ip (untagged) - Mouse programmed cell death 6 interacting protein (Pdcd6ip), transcript variant 3, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pdcd6ip
• Synonyms: AI480591; Aip1; Alix; AW544830; C76364; Eig2; mKIAA1375
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC026823 sequence for NM_011052
  CGGTCCGCTCCTCCGCTGCCGGTGCCGAGCGCGCCTAGGCCGTGGTCGCTGTCCCTCCGTCTGTGCGGCC CGACGCGGCGGCCGGGCGCTCACCATGGCGTCGTTCATCTGGGTGCAGCTGAAGAAGACCTCGGAGGTGG ACCTGGCCAAGCCGCTGGTGAAGTTCATCCAGCAGACGTACCCGAGCGGCGGCGAGGAGCAGGCCCAGTA CTGCCGTGCGGCCGAGGAGCTCAGCAAACTGCGGCGCTCGGCGCTCGGTCGCCCGCTGGACAAGCATGAG GGCGCCCTGGAGACGTTGCTGAGGTATTATGATCAGATTTGTTCCATTGAACCCAAGTTCCCATTTTCTG AAAATCAGATCTGCTTGACGTTCACGTGGAAGGATGCTTTTGATAAAGGTTCCCTTTTTGGAGGGTCTGT AAAATTGGCTCTTGCAAGCTTAGGATATGAAAAGAGCTGTGTGTTGTTCAATTGTGCTGCCTTAGCTAGC CAGATTGCAGCAGAGCAGAACCTGGATAATGATGAAGGATTGAAAACCGCTGCTAAGCAGTACCAGTTTG CTAGTGGTGCCTTTTTACATATTAAAGACACAGTGTTATCTGCCTTAAGTCGAGAGCCTACTGTGGACAT ATCTCCAGATACTGTTGGAACTCTCAGTCTTATTATGCTGGCTCAAGCTCAAGAAGTATTTTTCTTAAAA GCCACAAGAGATAAGATGAAGGATGCCATCATAGCTAAGCTGGCAAATCAGGCTGCGGATTACTTTGGCG ATGCTTTCAAGCAGTGTCAGTACAAGGACACGCTCCCCAAGGAGGTATTCCCCACCCTGGCTGCAAAGCA GTGCATCATGCAGGCCAATGCTGAGTACCACCAGTCCATCCTGGCCAAGCAGCAGAAGAAGTTTGGGGAA GAGATCGCAAGGTTGCAGCACGCAGCAGAACTGATCAAGAATGTGGCCTCTCGCTATGATGAGTATGTCA ATGTGAAGGATTTTTCTGACAAAATCAACCGTGCCCTTACTGCAGCAAAGAAGGATAATGATTTTATTTA TCATGACCGTGTTCCGGACCTTAAGGATCTGGATCCTATCGGCAAAGCCACACTTGTGAAGCCCACCCCA GTCAATGTACCTGTCAGCCAGAAGTTCACGGATTTGTTTGAGAAGATGGTCCCTGTGTCTGTGCAGCAGT CCCTGGCTGTGTTTAGTCAGAGGAAAGCTGACTTGGTCAACAGATCAATCGCTCAGATGAGAGAAGCTAC GACTTTGGCAAATGGAGTATTGGCTTCCCTTAACCTTCCAGCAGCAATTGAAGATGTGTCTGGAGACACT GTACCTCAGTCTATACTTACCAAGTCTACATCTGTAGTTGAACAGGGAGGCATCCAGACTGTCGACCAGC TGATAAAAGAGCTACCTGAGCTGCTGCAAAGAAATAGAGAAATATTAGAGGAGTCGCTAAGATTGTTGGA TGAAGAAGAAGCAACTGACAATGATTTAAGAGCAAAATTCAAGGACCGCTGGCAAAGGACTCCATCCAAT GACCTGTACAAGCCTTTACGAGCAGAGGGAGCCAAATTCAGAGCCGTTTTAGATAAAGCTGTGCAAGCGG ATGGACAGGTGAAGGAGCGCTACCAGTCCCATCGAGACACCATCGCACTTCTGTGTAAGCCGGAGCCAGA GCTGAATGCTGCCATCCCCTCTGCTAACCCAGCAAAGACCATGCAGGGCAGCGAGGTTGTAAGTGTCTTA AAGTCCTTATTATCAAATCTTGATGAAATCAAGAAGGAAAGAGAGAGTCTTGAGAATGACCTGAAGTCAG TGAATTTTGACATGACAAGCAAGTTTTTGACAGCTCTGGCCCAAGATGGCGTGATAACTGAGGAGGCTCT CTCTGTCACTGAGCTGGATCGGATCTATGGCGGTCTAACAAGTAAAGTTCAAGAGTCTCTGAAGAAACAA GAGGGACTTCTAAAAAATATACAGGTCTCACACCAAGAATTCTCCAAAATGAAGCAATCTAACAACGAGG CTAACTTGAGAGAAGAAGTTCTGAAGAACCTAGCAACTGCGTATGACAACTTTGTTGAGCTTGTAGCTAA CTTGAAGGAGGGCACAAAGTTTTACAATGAGCTGACTGAGATCCTGGTCAGGTTCCAGAACAAATGCAGT GACATAGTGTTTGCACGGAAGACAGAAAGAGACGAGCTCTTGAAGGATCTGCAGCAGAGCATTGCCAGAG AGCCCAGCGCTCCTTCAATCCCTCCTCCAGCCTATCAGTCCTCCCCAGCAGCGGGGCATGCAGCAGCGCC TCCAACTCCAGCCCCAAGAACCATGCCGCCTGCTAAGCCCCAGCCTCCAGCCCGGCCTCCACCTCCTGTG CTTCCTGCAAACCGAGTTCCTCCTGCTTCTGCTGCTGCTGCCCCTGCAGGCGTGGGGACGGCTTCAGCAG CGCCGCCACAGACCCCTGGCTCTGCTCCCCCGCCACAGGCTCAGGGACCACCATACCCTACCTATCCAGG ATATCCCGGGTATTGCCAAATGCCCATGCCCATGGGCTACAACCCCTACGCATATGGCCAGTACAATATG CCGTACCCACCGGTGTATCACCAGAGCCCCGGACAGGCTCCATACCCAGGACCCCAGCAGCCTACCTACC CCTTCCCTCAGCCCCCGCAGCAGTCCTACTATCCACAGCAGTAACGCTGCCACGTGCTGCTGGTTCAGAT CAGAGCGACAGGACAGCAGCTGCCACCAGCTCTAAGCCACGCTCTGGCCACTCGAGAGTATCTTGCTCTA TTGATTGCTGTGGATGATTTCTGTCTGTGGCTAAAGCCGAAGGCTGGGCCCCACCTCCACATTTGATCGC ACTCGTGAGATTCTGCTGCTGTTGCAGTATAAACGCTAGCTATAATAGCATTTGAAAAAAATTACAGTTC CATAAAATGCTGAAAATGAGAAATTAAACCTGCAAGTGAAACATTTGAAATTAGCATACTTTATAAGATG CAGTTGGGACAAAGATGGCTTAAGTACTGATATTTAAGGAAAAAGTTTTCTTTCTCTTTTGGTTTATTGA TTTAGTTTAATTTCTATTATGATATTTTGCATAATCAAGGCATTGTAAATCTTATAATTTAAAAATAAAT TACTTACGAACAGTTAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_011052
• Insert Size: 2610 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC026823, AAH26823
• RefSeq Size: 3181 bp
• RefSeq ORF: 2610 bp
• Locus ID: 18571
• UniProt ID: Q9WU78
• Cytogenetics: 9 F3
• Gene Summary: Multifunctional protein involved in endocytosis, multivesicular body biogenesis, membrane repair, cytokinesis, apoptosis and maintenance of tight junction integrity. Class E VPS protein involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome. Binds to the phospholipid lysobisphosphatidic acid (LBPA) which is abundant in MVBs internal membranes. The MVB pathway requires the sequential function of ESCRT-O, -I,-II and -III complexes. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis. Adapter for a subset of ESCRT-III proteins, such as CHMP4, to function at distinct membranes. Required for completion of cytokinesis. May play a role in the regulation of both apoptosis and cell proliferation. Regulates exosome biogenesis in concert with SDC1/4 and SDCBP (By similarity). By interacting with F-actin, PARD3 and TJP1 secures the proper assembly and positioning of actomyosin-tight junction complex at the apical sides of adjacent epithelial cells that defines a spatial membrane domain essential for the maintenance of epithelial cell polarity and barrier (PubMed:27336173).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. This results in a shorter protein (isoform 3), compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.