Trnt1 (NM_027296) Mouse Untagged Clone

CAT#: MC201476

Trnt1 (untagged) - Mouse tRNA nucleotidyl transferase, CCA-adding, 1 (Trnt1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Trnt1
• Synonyms: 2410043H24Rik; 2610044E04Rik; 9830143O18Rik; AA408384; C76540; CGI-47; mt-Trat
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC031764 sequence for NM_027296
  GCGGCCCGGGCAGCAGGCGGATGTGTGATTGGCGACTTGTCTCCAGATGCAGAGCGTCCTGTATCCTTGG CACAGGCAGGTCCTGAGGTGTAGTTGGAGTAGATTGTGCCTTCTGAAGCGATACCTGTTTACAATGAAGT TGCAGTCCCCAGAATTCCAGTCCCTTTTCACCGAAGGATTGAAGAGCCTGACAGAATTATTTGCCAAAGA GAATCATGAATTAAGAATAGCAGGAGGAGCAGTGAGGGATTTACTGAATGGGGTGAAGCCTCAAGATGTG GATTTCGCCACCACTGCCACCCCTACTCAGATGAAGGAGATGTTCCAGTCAGCTGGCATTCGCATGATCA ACAACAAAGGAGAGAAACATGGGACTATCACTGCCAGGCTTCATGAAGAAAATTTTGAAGTTACTACACT CCGAATTGATGTTACCACTGATGGAAGACATGCAGAGGTAGAATTCACAACTGACTGGCAGAAAGACGCT GAACGAAGAGATCTCACTATAAATTCTATGTTTCTAGGTTTTGATGGTACACTGTTTGATTACTTTAATG GTTATGCAGATTTAAAAAATAAAAAGGTTCGATTTGTTGGACATGCAAAACAGAGAATTCAGGAAGATTA TCTTCGAATTTTAAGGTATTTCAGGTTTTATGGCAGAATTGTCGACAGACCTGGTGACCATGACCATGAG ACTCTAGAAGCAATTGCAGAAAATGCCAAAGGTTTGGCTGGAATATCAGGAGAGAGGATTTGGGTAGAAC TGAAGAAAATTCTTACTGGTGACCATGTAAACCATTTGATCCACCTGATCTATGATCTTGGTGTGGCTCC TCACATAGGTTTACCTGCTAATGCAAATTTAGAAGAATTTAACAAAGTCAGTAAAAATGTTGAAGGCTTT TCACCAAAACCAATGACTCTTTTGGCTTCTTTATTCAAAGTACAAGATGATGTCACAAAATTGGACTTGA GATTGAAAATTTCAAAAGAGGAGAAAAACCTGGGTTTATTTATAGTTAAAAACAGGAAAGACTTGATTAA AGCAACAGATAGTTCAGAACCATTGAAACCATACCAAGACTTTGTGATAGACTCTAGGGAACCAGATGCA ACTGCCCGTGTTTGTGAGCTGCTGAAGTACCAAGGAGAGCATGGTCTTCTCAAAGAGATGCAGCAGTGGT CTGTCCCGCCGTTCCCTGTGAGTGGACATGACATCAGGAAAGTGGGTATTTCTTCAGGGAAGGAAATTGG GGCCCTGCTACAGCAGTTGCGTGAACAGTGGAAGAAAAGTGGTTACCGGATGGAAAAAGATGAACTACTA AGTTACATAAAGAAGACCTAAAGATGAGCCCTGTATGAAACAGGGGAAGTCTTGAAAGTTCATCTAAAAC TAAATGAAGAGTCAACAAGAAAAGTAAACAGCTGTATTACCCGGGAAACCAGCTGTGCACTGTAGAAGGC CGGCCTATCAGATTCAGGTTGGACGCTTTATACACAGTCAGTGTCTCATGTACATATCCATGAGGATTCA CATATGCTGCCATTGGACCACATAAGGATAAACTACAGACAAAGAAATAATGTGGAGAATAAGACAATGA AAAGAAAAATAGTAATCCCAGTTTTTTACTTATTTGGGACATAAGTTATGCTTTTTAACCTTTGAATTTT AGATATTATATAGTACTTTGTGTAAAATGTAAGACAAGTATACAGGAGGTTGGCAGATATTCTGTTGCTT AAAAATGTTCTTTGATGATTCAAAGGTAAATATATGTGTTTACAAAGCCTAGTGAGGTCATGTTTTCATC TTTGGACATTTAGTTACTTTACCTACATATCAGTTTCCTGACCTTCTTGATCTGTAGTTCTAATGAATTC ATCAAAGTGTTAAGCTTCACAGCATCAGCACATGTTCAGAGACCCACCCAATTTTGGTCCCGTGTAGCAG AACTAGCTGAAGTATAATGCTTGAAGCATTCCTATTATGCTTTAAGATAGGATGTGTCTAAACAAAAATT GAGGGGAAAAAGACTGTATTACAGATGTTTCCAGGCTGTAAGACAGCTTGCCCTGTGTTTATCTCCTTTA CTGAGATAAACGCATATTTTTTAGGCACTGGAGAAAATATGGAAAGTGGAACCACAGAAGGGAATATGGA AATATATTAAAAATGTAATAAAGATCTTTCCTAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_027296
• Insert Size: 1305 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC031764, AAH31764
• RefSeq Size: 2240 bp
• RefSeq ORF: 1305 bp
• Locus ID: 70047
• UniProt ID: Q8K1J6
• Cytogenetics: 6 E1
• Gene Summary: Adds and repairs the conserved 3'-CCA sequence necessary for the attachment of amino acids to the 3' terminus of tRNA molecules, using CTP and ATP as substrates.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 both encode the same isoform (a).