Myl12b (NM_023402) Mouse Untagged Clone

CAT#: MC201459

Myl12b (untagged) - Mouse myosin, light chain 12B, regulatory (Myl12b), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Myl12b
• Synonyms: 1500001M02Rik; C77744; Mylc2b; RLC-B
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC028878 sequence for NM_023402
  CTCTCGGGTGTGCAGCATCAGGTCAGATTTAAACCGCCACCATGTCGAGCAAAAAAGCGAAGACCAAGAC CACCAAGAAGCGCCCTCAGCGCGCAACCTCCAATGTGTTCGCCATGTTTGACCAGTCCCAGATCCAGGAG TTCAAAGAGGCCTTTAACATGATTGACCAGAACCGGGATGGCTTCATTGACAAGGAGGACCTGCACGACA TGCTGGCGTCTCTGGGGAAGAATCCCACTGATGCCTACCTGGACGCCATGATGAACGAGGCCCCGGGCCC CATCAATTTCACCATGTTCCTCACCATGTTTGGGGAGAAGCTGAACGGCACTGACCCCGAGGACGTCATC AGAAACGCCTTCGCTTGCTTTGATGAGGAAGCCACAGGCACCATCCAGGAGGATTACCTGAGGGAGCTGC TGACCACCATGGGCGACCGCTTCACAGACGAGGAAGTGGATGAGCTGTACAGAGAGGCCCCCATTGACAA AAAGGGGAACTTCAACTACATTGAGTTCACACGCATCCTGAAGCACGGCGCGAAAGACAAAGATGACTGA AGAGCTGCGGCGGCTGACACGCCTCTTGCGTTTGCTTGCTCACTTCCTGTCTCAGACACACACACCCCAT AGGACCTACTGCGTGCCACTTAGTTTCTCAGTCACTTCCCCTCCCCCATAGGGCCTACTGTGTGCCACTT AGTTGAGAGCTTTTTTTTTAAATGTATTTATTCCAGGCCCTTTCTGTCACTTAGCATGTGCATAATCAGA CTGGAACGGGGGACAATGTTGTAAATTGTACTGAAAACGAGATGCAAATAAAAAATGAACCACTGTGAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_023402
• Insert Size: 519 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC028878, AAH28878
• RefSeq Size: 896 bp
• RefSeq ORF: 519 bp
• Locus ID: 67938
• UniProt ID: Q3THE2
• Cytogenetics: 17 E1.3
• Gene Summary: Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Phosphorylation triggers actin polymerization in vascular smooth muscle. Implicated in cytokinesis, receptor capping, and cell locomotion.[UniProtKB/Swiss-Prot Function]