Rab7b (NM_145509) Mouse Untagged Clone

CAT#: MC201452

5430435G22Rik (untagged) - Mouse RIKEN cDNA 5430435G22 gene (5430435G22Rik), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rab7b
• Synonyms: 5430435G22Rik
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC019395 sequence for NM_145509
  CTCAGAGGGAAGTGGCCTCTCACCAAAACCGTTTGCGTTCTCAATACTGGCCAGATTGAAACTGCCACTC CTAGAAGAGAGACCCAGACCTAGAACTCCCACCTGTGTGAGGCCCTGAAGCCCCTCATGAATCCCCGAAA GAAAGTGGACTTGAAACTTATCATTGTTGGTGCACTTGGCGTGGGAAAGACCTCTCTTCTTCACCAATAT GTACACAAAACATTTTTCGAGGAATACCAGACCACACTGGGGGCCAGCATCCTCTCCAAAATCATCATAT TGGATGACACAACTTTGAAGCTGCAGATCTGGGACACAGGTGGTCAGGAGCGGTTCCGCTCAATGGTATC AACATTCTACAAAGGTTCCGATGGCTGTATCCTGGCATTTGATGTTACTGATCCAGAGTCCTTTGAAGCC CTGGATATCTGGAGGGATGATGTCCTGGCAAAGATTATCCCTATGGAGCAGTCATATCCCATGGTGGTGC TGGGGAACAAAATCGATCTGGAAGATAGGAAGGTGTCCCAGGAGGTGGTCCATGGATGGTGTAAAGAGAA GGATATGCCATATTTTGAAGTTAGTGCGAAGAATGACATCAATGTGGTACAAGCCTTTGAGGTTCTGGCC AGCCGGGCTCTGTTGAGGTACCAGGGCACCGCAGAGAACCACCTCATAGACTCCATCAAGCTCTCTCCAG GCCAGCCAAAGAGCAGATGCTGCTGATCCTGGCCTCCCTCCGGACTCCCGATGACAGCTGTGCAGCCATT CCAAGTGCAATCCCACCAGCCGGTACTCAGCTAGGAGGCTGGGGCACTGCAGCAGGAGAGAATGTCACAG GCAAATCCTGGTCCCAGAGAAATCACGGGCGTCTCCATGACTCAAGCTCCTCTTTCTCCTCTGCCTCAAC ACCGTCCTGCTCATCTCTGCAGACAAACTGCTCCCTTCAGCACCATGGACCGACAGGTTTGGATGGAGAT GCAGTTCTGATTCCCTCAGCAGCCAGAACATGGACCGCTCCTGACAGGCAGAAGCCCACAGGCCTGAGAC TTCCCACAGCTTCTTTCAGGGAGAGCTGAGGGAGCAAGATAAAAACAGTTGTACCTATTTCCTGGGTGCT CAAACAGCTCCCATAACTGACCAGGCCTCAGTGCGCCCTCTGCCAAATGAGAGTTCCCAAGGCTTCCCCA TATCCTAGGGCGACTGAGAACCATAATCTCATTTAAATCCCACGTGCTGGTGTGGACACACATGCTCACT CCTTTGGAGAGTATTTTTGATGCCTTCGATGTACATTGCACTGAAGTCACCAGACACACAAAGCAGACAT GAGTCCTTCACTCCCAGAAGCGCACCTGGAGAGAGAGGGCCAGCAGACATGAGCAAGACAGACAACAGCA ACGAGGTACTGTTTTAGACAAAGGGGCCAAGAGGGCTTTCCTGTGACGGTGACCGTTGGGCTGGCAGCTC CAGGAAGAGCTACAGGAAGAACATGCCAGGAGAGGGAGCAGCTAGGCCAAGGCTCTGAGGCAATGGGCAG AGTGCTGCATTCCATTCCCTGAGGACTCAAAGCTGCTCAGAGAAAAAAAGGCCAGGGATGTCCTGTCCCC AGAAGGTACTTTGATCATTTGATGACTGGCCACTAGGGGGCAGAAGTGTCCTCTGCTTTTAGGCTGAGGA GTTCTGGTAACACTTGAAAAGCTGGTGTTTGTGTCAAGAAAATGGCTGAAGCTTGTTCCCAGGCTGTAGG AATGTTGAACCTGGGCTAGTTGGGTTAAAAAAAAAAAATCAAATGTCTTGATTCAGGCAACAAATCGAAA TCAGAGACAGATCCCAGAGCGAGGTACTTGGAGAGCTGTGGATTCTGTGTCCTGTTCATTTCAAAGCTAG AACCTATTTTTTTTTCTTCTGGCTTCACACTGCACAGATAGGGTGGCTCATTCTGAAGGAGGTGGACAGA ACGAACTTTCAGGGTCTTCAATGGTCAGGTTCATACCTCATGCCTTCCACAGACATTCCTGTCTCCACAG AAGTCTTCTTTCCTGAGTTCCAAGCAGATCCGAGGTGCCAATCTCTTACACGCATTCTCACGACCGGCCA GGAAGAACGCAGCAAACCAGAATCTTCTGCGGCAAAACTTTATTGCTTACATCTTCAGGAGCAAGAGTGC AAGAGAGCAAGAGCTAGAAAGCAAGAAAAAGAACAAGAACAAGAAAGCAAGAGCAAGAGAGAGAAAAAGC AAGAACAAGAGAGAGAGAAAGAATGGCAAAACCCCGTCCCTTTTAAGGAGAATTATCCTCCGCCTAGGAC GTATTACTCCCTGATTGGCTGCAGCCCATCGGCTCAGTTGTCATCACGAGAAAGGCAGAACACATGGCGG GAAAACTGCCCCTGCACGTGTGCAGATTATGTTTACTACTTAGAACACAGCTGTCAGCGCCATCTTGTAA TGGCAAATGTGAGGGCGGCTCCCAACATCAATCTCTAGGAAGCCAGTCAGGGGGTACTAACTGGGGGTCT GGGGGCTCTGTCTGCACCCGCCTAGCTCTGAGGTATAAAGCAACTTACTAAGGACAGCAGTCACAGGAAC AGTGAGCCCTGCGTCCTTCAGAGATGAACACTGTGCCCCCGTCACACATTGGTGTGGCTCTCCTGAAGTC AGACTGTGGAGGGATGTGTGTGTGTGTGGGGGTGCATTCGGCAGCACAATGAACATAAGGACCATATCTA AGGGTACGCAGCAAATAGTATGTGTTTCCATTGTTTATATCCTTTATGGGACAATAGTCCCTCTTGGTCA GTGATACCAGTCTCAGTTACCCATGGTCAACTGTGGACCAAAAATATTAAATAGCAAATCCCAGAAATAA ATAACTTACAACTTTCAATGCAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_145509
• Insert Size: 987 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC019395
• RefSeq Size: 2909 bp
• RefSeq ORF: 987 bp
• Locus ID: 226421
• UniProt ID: Q8VEA8
• Cytogenetics: 1 E4
• Gene Summary: Controls vesicular trafficking from endosomes to the trans-Golgi network (TGN). Acts as a negative regulator of TLR9 signaling and can suppress TLR9-triggered TNFA, IL6, and IFNB production in macrophages by promoting TLR9 lysosomal degradation. Also negatively regulates TLR4 signaling in macrophages by promoting lysosomal degradation of TLR4. Promotes megakaryocytic differentiation by increasing NF-kappa-B-dependent IL6 production and subsequently enhancing the association of STAT3 with GATA1. Not involved in the regulation of the EGF- and EGFR degradation pathway.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).