Smarcal1 (NM_018817) Mouse Untagged Clone

CAT#: MC201448

Smarcal1 (untagged) - Mouse SWI/SNF related matrix associated, actin dependent regulator of chromatin, subfamily a-like 1 (Smarcal1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Smarcal1
• Synonyms: 6030401P21Rik; AI851716; Mharp
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC029078 sequence for NM_018817
  CAGGTCTCAGCAAAGAGGAATGTCTATGTGGTGGTGTGCATTTGTGGTTTATGGTTTATTAGTTGGACCT CTCATTCCGTGGTCTCATCTCCCCAACTGGGTCAGTCAGCATACCAGGCCTTGCTTTTACCAACTTCCCA ACTAAAGGGTGACATCCCTGCATGAGCCTATCCTGTTAAAAATGTCCTTGCCACTTACAGAGGAGCAGAG GAAAAAGATTGAAGAGAATCGGCAAAAAGCTTTGGCCCGTCGAGCTGAGAAGTTGTCAGAACAGCCACAG AGCACAGCTTCAGGCTCCTCTGCTGCCGGCCCGTCCCAGTCCAAGCAGGGCTCTCTCCTGAATCTCCTAG CGGAGCCTTCAAAGCCAGTGGGCCATGCCTCCATTTTCAAGCAACAGAATCTCAGTAATTCACTTCCTAC TGATCAGAGACCTCACAGCTCCCGCTGTTCTCAGCCCAGCCCAGCAGAGGAAACGACGGGGCTATGGAAG ACCCAAGGAGAGATGTCCACAGCCTGCCCAAAGCCTAACCCAAGTCCTCCAGGAGCCTCTAACCAACCAC TTTTGGGTTATAAATCCAGCGAGGGTCAACCCCAGGCAACCTGGGACACAGGAGCTTCCTCTTCTGGACC ATTCCCCAGGGACCCCGAGTTAGAGGCCAAGGCAGCAAGGCCTTCCACCTCTAGGCAGAGCATTTCGGAC AGCTTTTATGTGTTAGGGGGAAAAACACCTAGGACAGAAGGGAGACCCCCAAACATACTGCAAACCACAC CCCAAAACACAGGCTTTCTACGGGGAGCGTGCATAAAGACTGGGGATCGCTTCCGGGTAAAGATCGGCTA CAACCAAGAGCTCATTGCCGTGTTTAAGTCTCTGCCCAGCAGACACTATGATTCTTTTACAAAGACCTGG GACTTCAGCATGAGCGACTACAGAGCCTTGATGAAGGCAGTCGAGCGACTCTCCACGGTCTCCCTGAAGC CACTGGACGAGGCTGGTGGCAGTGTTGGAGGACAGACCAGCCTTCCCTCAGCTCCATCCCTGACCTTTGT GACAGGGAAGTGCATGCTCATCTCTCGAGTCCGCTTCGAGGTTGACATCGGCTATTCGGAGGCGGTGATT GGCCTGTTTAAACAGATGGAGTCCAGGAGTTACGATATCAAGACCAGGAAGTGGAGTTTTCTCTTGGAAG AGCACAATAAACTAATTGCAAGGTCACGTGAACTGAAGCAAGTTCAGCTGGACCCACTGCCCAAGACAGT GACCCTGGCATTTGCATCTCAGCTGGAGAAAACGTCTCCCAAACTGAAAGCAGACGTGCCAGAAGCGGAC CTTTCCGGAGTGGATGCCAAGCTTGTGTCTAGCCTCATGCCCTTTCAGAGGGAGGGAGTTAGCTTTGCCA TATCAAAAAGAGGCCGCCTGCTACTTGCCGATGACATGGGCCTAGGGAAGACCGTCCAAGCCATCTGCAT AGCAGCTTTTTACCGAAAGGAATGGCCACTGCTGGTGGTCGTGCCTTCCTCTGTGCGCTTCACCTGGGAG CAGGCCTTTCTTCGGTGGCTTCCATCTCTTAGCCCAGAGAACATCAACGTGGTGGTGACCGGGAAGGGCC GCCTAACAGCTGGCTTGGTCAATATTGTCAGCTTTGACCTCCTTTGCAAGTTGGAGAGGCAGCTAAAAAC CCCTTTCAAAGTCGTCATTATTGATGAATCACACTTCCTCAAGAACATAAAGACTGCCCGCTGCCGAGCA GCTGTGCCTATCCTAAAGGTTGCCAAGAGGGTGATCCTACTGTCAGGCACACCCGCCATGTCGCGGCCCG CAGAGCTCTACACACAGATCATTGCTGTCAAGCCAACCTTCTTCCCTCAGTTTCATGCCTTTGGACTCCG CTACTGTGACGCCAAACGGCTCCCTTGGGGCTGGGACTACTCAGGCTCCTCCAACCTGGGCGAGCTGAAG CTGCTGCTAGAGGAAGCAATCATGCTGCGGCGCCTCAAATCCGACGTGCTCTCCCAGCTTCCGGCCAAGC AGCGCAAGATGGTGGTGGTCAACCCTGGTCGGATCAGCTCCAGGGCTAAGGCAGCCCTGGATGCAGCAGC CAAAGAAATGACCAAGGACAAAACTAAACAGCAACAGAAAGAAGCTCTCCTTGTCTTCTTCAACAGGACA GCAGAAGCTAAAATCCCATGTGTCGTCGAGTATATCCTGGACCTTCTGGACAGCGGACGAGAGAAGTTTC TAGTGTTTGCACACCATAAGGTGATTCTGGATGCAGTTGCCAAGGAGCTCGAGAGGAAGAACGTTCAACA CATCCGGATCGATGGCTCCACTCCCTCAGCAGACCGGGAGGCTCAGTGCCAGCGGTTCCAGCTGTCCAAA GGGCACACAGTGGCACTGTTGTCCATCACAGCCGCCAACATGGGCCTCACCTTCTCCACAGCTGATCTGG TCGTGTTTGCTGAGCTCTTCTGGAACCCAGGGGTGCTGATCCAGGCCGAGGACCGTGTGCACCGCATTGG ACAGACAAACTCTGTGAGCATCCACTACCTGGTGGCAAAAGGCACAGCAGATGACTACCTTTGGCCTCTG ATTCAAGAGAAGATTAAAGTTCTGGGGGAAGCTGGGCTTTCTGAGACCAATTTCTCGGAAATGACGGAAG CTACAGACTACGTGCACAAGGACCCAAAGCAGAAGACGATCTATGACCTGTTCCAGCAGTCCTTTGAAGA CGATGGAAATGACATGGAATTCCTGGAAGCAGCAGAGTCCTTTGAGCTAGGAAGTACTTCAGGAACCTCT GGAAACATTTCCCAGGATCTGGGAGATCTCTTAGATGAAGACGAAGGCAGTCCGCCAAAGAAAAGCAGAT TTGAATTCTTTGATAATTGGGACAGCTTTTCCTCTCCCTTTTGACAGGGTCAGAAAAGGAAAGAAACGAA AGAAAGCAGTCACAAAATCATGGGGCTCATTGAAATAAAGTATTTTCTTTAAAAAAAAAAAAAAAAAAAA AAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_018817
• Insert Size: 2733 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC029078, AAH29078
• RefSeq Size: 3015 bp
• RefSeq ORF: 2733 bp
• Locus ID: 54380
• UniProt ID: Q8BJL0
• Cytogenetics: 1 36.72 cM
• Gene Summary: ATP-dependent annealing helicase that binds selectively to fork DNA relative to ssDNA or dsDNA and catalyzes the rewinding of the stably unwound DNA. Rewinds single-stranded DNA bubbles that are stably bound by replication protein A (RPA). Acts throughout the genome to reanneal stably unwound DNA, performing the opposite reaction of many enzymes, such as helicases and polymerases, that unwind DNA. May play an important role in DNA damage response by acting at stalled replication forks (By similarity).[UniProtKB/Swiss-Prot Function]