Smg8 (NM_024262) Mouse Untagged Clone

CAT#: MC201444

Smg8 (untagged) - Mouse RIKEN cDNA 1200011M11 gene (1200011M11Rik), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Smg8
• Synonyms: 1200011M11Rik
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC020005 sequence for NM_024262
  GCTAGAGGAAGCTCTTTCTCCGCCATGGCGGGGCCGGTCAGCTTGCGCGAGCTGCTCATGGGGGCGTCGG CCTGGCTGGGCTCTGAGAGTCCTGGAGGCCCCTCCGCTGAAGGCGGGGGAAACGCGGCTGGCACGCCCGA GCCCCCGTGGAGGGAGGATGAGATCTGCGTGGTGGGAATCTTCGGCAAGACGGCTCTGCGCCTCAACTCC GAGAAGTTCTCTCTGGTGAATACTGTGTGCGATCGACAGGTTTTCCCACTCTTTCACCATCAGGATCCTG GAGATCCAGGAACTGGAATCAAGACCAAAGCTGTCTCTGTCGGAGAGGCGGGAGGAGCCGGAGACCCCGG GGCTGCAGCCGGGGATTCACTTCGCGGAGGTATGGCTACGGCGGAAGGTAACCGAGCTGAGCCAGGTCCC CAGGACTTCAGTCTTTTGCAGGCCTATTACAATCAGGAAAGCAAAGTTCTGTATCTTATTCTCACTTCCA TCTGTGACAACTCACAGCTTCTGCGTGCTTGTCGGGCCCTCCAGAGCGGTGAAGCCGGAGGGGGCCTCTC TTTACCTCATGCAGAAACACACGAGTTCTGGAAGCATCAGGAGAAGTTACAGTGTCTCAGCCTCCTTTAC CTCTTCTCTGTCTGTCATATCTTGCTCCTGGTCCATCCGACGTGTTCCTTTGACATCACTTATGATCGTG TATTCAGAGCCCTGGATGGACTGAGACAGAAAGTACTACCCCTCCTCAAAACAGCCATTAAGGATTGCCC AGTTGGCAAAGACTGGAAGTTAAACTGCCGACCTTGCCCACCTAGACTCCTCTTCCTCTTTCAACTCAAT GGGGCCCTTAAGGTGGAACCGCCTCGGAGCCAGGATACAGCTCATCCAGATAAACCCAAGAAGCACTCTC CCAAAAGAAGGCTGCAGCATGCCCTGGAGGACCAGATCTATAGAATCTTTCGCAAGAGTCGAGTCTTGAC TAATCAGAGTATCAACTGCCTCTTTACGGTACCTGCCAACCAAGCTTTTGTGTACATAGTCCCCGGAAGC CAGGAGGAGGACCCAATTGGCATGTTGCTGGACCAGCTTAGGAGCCATTGTACCGTGAAAGACCCAGAAT CGTTGTTGGTGCCCGCACCCCTTTCTGGACCTAGGCGGTACCAAGCGATGAGACAGCACAGCCGCCAACA GCTCTCCTTCCACATTGATAGCAGCACCTCGAGTTCTTCAGGCCAGCTAGTGGATTTCACTCTTCGAGAA TTCCTGTGGCAGCACGTGGAGCTGGTCCTAAGCAAAAAAGGTTTTGATGACAGTGTGGGCAGGAACCCAC AGCCTTCCCATTTTGAACTGCCCACTTACCAGAAGTGGATCTCTGCAGCAGCAAAGTTGTATGAAGTGGC TATCGATGGGAAGGAAGAGGACTTGGGCTCCCCCACTGGGGAGCTAACATCTAAGATTTTAAGCAGTATT AAAGTCTTAGAAGGGTTTTTGGATATAGACACAAAATTCTCGGAAAACCGGTGCCAAAAAGCTTTACCCA TGGCTCATAGTGCCTACCAGTCAAACTTGCCGCATAATTACACCATGACAGTCCATAAGAATCAGCTTGC TCAGGCTCTTCGGGTATACAGTCAGCATGCTCGAGGTCCAGCCTTTCACAAATATGCCATGCAATTACAC GAAGACTGCTACAAATTTTGGAGCAATGGCCATCAGCTGTGCGAAGAGAGGAGCTTGACTGACCAACACT GTGTCCACAAATTTCATTCCTTACCTAAATCAGGAGAAAAGCCAGAGGCAGATAGGAACCCTCCTGTGCT CTACCACAATAGCCGAGCCCGATCCACCGGAGCCTGCAACTGCGGAAGGAAGCAGGCACCTCGAGACGAC CCCTTCGATATCAAGGCTGCTAACTATGACTTTTACCAGCTTCTGGAAGAAAAATGTTGTGGGAAATTGG ATCATATCAATTTCCCTGTATTTGAGCCAAGTACTCCAGATCCCGCTCCTGCTAAAAATGAACCCTCTCC TGCCCCGCCAGACTCGGACGCCGAGAAACTGAAGGAGAAAGAACCTCAAACCCAAGGAGAGAGCACAAGC CTCAGCTTAGCTTTGAGCTTGGGCCAGTCAACAGATAGCCTAGGTACCTACCCCGCTGATCCACAGGCAG GAGGAGACAACCCAGAAGTCCACGGGCAAGGAGAAGGAAAGTCTGAGAAGAGACCAAACTTGGTTGACCG TCAGGCATCCACAGTTGAATATCTCCCAGGCATGCTACATTCAAATTGCCCCAAAGGTCTCCTACCCAAA TTCTCCAGCTGGTCTTTGGTTAAACTAGGCCCAGCCAAGTCTTACAACTTTCATACAGGTTTGGACCAAC AGGGCTTCGTTCCAGGAACAAACTATCTTATGCCTTGGGACATTGTCATCAGGACTAGAGCTGAAGATGA AGGAGACTTAGACACGAACTCCTGGCCTGCTCCAAATAAAGCTATTCCTGGGAAGAGAAGTGCAGTTGTG ATGGGCAGAGGAAGGCGGAGGGATGACATAGCTCGAGCCTTTGTTGGCTTTGAGTATGAAGACTCTCGAG GTCGGAGGTTCATGTGCTCAGGGCCTGACAAAGTAATGAAAGTGATGGGAAGTGGACCCAAGGAGTCGGC TTTAAAAGCCCTCAACAGTGACATGCCCCTGTACATCCTGTCCTCGTCGCAGGGCAGAGGGCTGAAGCCA CATTATGCTCAACTTATGAGGCTTTTTGTTGTGGTTCCTGATGCTCCTTTGCAGATAATACTGATGCCTC AGGTTCAACCAGGCCCACCACCATGTCCAGTATTTTACCCAGAAAAACAAGAAATCACTCTCCCACCTGA TGGCCTTTGGGTCTTGAGATTTCCTTATGCCTATGTGACTGAGAGAGGACCTTGTTTCCCTCCCAAGGAG AATGTGCAGCTCATGAGCTACAAGGTGCTCCGAGGAGTCCTCAAAGCAGTTACACAATGAGTCTTCCAGC CACTCTGCCGCAGACTGAAGCTGCTGTTTGTCTTGTTTTTGATGTGTTTGGGTTTTTTTTTAATGTGTAT ACTGTGTCTTCCCAAAACCCAATGTGTTTTGGTTACAAGTATTCAGTATTTGGACAATAATTGTTCCATT ATTTCATTATATTGATATTTATTGTAAAATCAATAAAAGTTTGTTCTAGAAAAAAAAGGATACAAAATGT AAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_024262
• Insert Size: 2976 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC020005, AAH20005
• RefSeq Size: 3236 bp
• RefSeq ORF: 2976 bp
• Locus ID: 74133
• UniProt ID: Q8VE18
• Cytogenetics: 11 C
• Gene Summary: Involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Is recruited by release factors to stalled ribosomes together with SMG1 and SMG9 (forming the SMG1C protein kinase complex) and, in the SMG1C complex, is required to mediate the recruitment of SMG1 to the ribosome:SURF complex and to suppress SMG1 kinase activity until the ribosome:SURF complex locates the exon junction complex (EJC). Acts as a regulator of kinase activity (By similarity).[UniProtKB/Swiss-Prot Function]