Rnf128 (NM_023270) Mouse Untagged Clone

CAT#: MC201428

Rnf128 (untagged) - Mouse ring finger protein 128 (Rnf128), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rnf128
• Synonyms: 1300002C13Rik; AI987883; GRAIL; Greul1
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC010477 sequence for NM_023270
  CCCACGCGTCCGGCTTGCTTGCAGGAGCTGCGTCTGCAGTAGCCTGGCCGCTGACGCTGCGTGCCGGCTG GCAGGGCAGCCTGCGACCTCGCTGGCCCCGCGCCCGCTGCTAGCCGCCGGCTCCCCACCTGGTTCGCACC TAGTCCCAGCCCGGGTCGCCTGCCGAGTGCGCGCCATGGGGCCGCCGCCCGGGATCGGGGTCTACTGCCG CGGCGGCTGCGGAGCTGCCCGGCTACTGGCTTGGTGCTTCCTTCTGGCTCTGAGTCCGCACGCGCCCGGT TCCCGGGGAGCCGAAGCCGTGTGGACCGCGTACCTCAACGTGTCCTGGCGGGTTCCGCACACCGGAGTGA ACCGCACGGTGTGGGAGCTGAGCGAGGAGGGCGTGTACGGCCAGGACTCGCCGCTGGAGCCCGTCTCCGG GGTCCTGGTACCGCCCGACGGGCCCGGGGCGCTCAACGCCTGTAACCCGCACACCAATTTCACGGTGCCC ACGGTTTGGGGGAGCACGGTGCAAGTATCTTGGTTGGCCCTCATCCAACGCGGTGGAGGCTGCACCTTCG CGGACAAGATCCATCTGGCTTCAGAGAGAGGGGCTTCTGGAGCGGTCATCTTTAACTTCCCTGGGACCCG CAATGAGGTCATCCCCATGTCTCACCCGGGTGCTGGGGACATTGTTGCAATCATGATTGGCAATCTGAAA GGAACAAAAATTCTGCAGTCTATTCAAAGAGGCATCCAAGTCACAATGGTCATCGAAGTAGGGAAAAAAC ATGGCCCTTGGGTGAATCATTATTCAATTTTCTTCGTTTCTGTGTCCTTTTTCATAATTACGGCAGCAAC CGTGGGCTATTTCATCTTTTATTCTGCTCGAAGATTACGAAATGCAAGAGCTCAAAGCAGGAAGCAGAGG CAGTTAAAGGCAGATGCTAAAAAAGCTATTGGAAAGCTTCAGCTGCGCACCTTGAAACAAGGAGACAAGG AAATTGGCCCTGATGGAGATAGCTGTGCTGTGTGCATTGAGCTCTATAAGCCAAATGATTTGGTGCGCAT CCTAACCTGCAATCATATTTTCCATAAGACATGTGTGGACCCGTGGCTTTTAGAACACAGGACTTGCCCC ATGTGCAAGTGTGACATTCTCAAAGCTCTGGGAATTGAGGTGGATGTTGAAGATGGATCAGTGTCTTTAC AAGTTCCTGTTTCTAATGAAGCATCTAATACTGCCTCTCCCCATGAAGAGGACAGTCGCAGTGAGACTGC ATCATCTGGATATGCTTCAGTACAAGGAGCAGATGAGCCACCTCTGGAGGAACATGCGCAGTCAGCAAAT GAAAATCTACAGCTGGTAAACCATGAAGCAAATTCTGTGGCCGTGGATGTTGTTCCCCATGTTGACAACC CAACCTTTGAAGAAGATGAAACTCCTGATCAAGAGGCAGCTGTTCGGGAGATTAAATCTTAAAAATCTGT GTCAATAGAAAACTTGAACCGTTAGTTAACAACAGGACTGCCAATCAGGGCCTAGTTTACTATGAATGAA CTGGGTAAACGTAAAACAAGAATGATACTGAAAGTGCTGAGGTAACTTATATTATACTATAGTTAAATGG CTTAACATATTTACCCCAGTACCGTTTTCCACAAACTCACCATAACGTTTTTCATAGGCAAGTTTCCTCT TGGTGATAGTGATAGCAACATTTTTAACATTCAGAACCGTCTATGAGTAGTCAGGTTTTTCATTTACAAC AACTTTGTTATAAAAAAATATGTTGCTTTAAAAGTGTGGAGTAGCTGTAATCACTTTGTTTTATGATAGT ATCATAATTAAACAATACTACTACTTTAGCTTGGGCTCTGTGTGTCGGGGTTTGTCTCCAGGTGCTTATA TTGATCTGGAATTTGTTTAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_023270
• Insert Size: 1287 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC010477, AAH10477
• RefSeq Size: 1928 bp
• RefSeq ORF: 1287 bp
• Locus ID: 66889
• UniProt ID: Q9D304
• Cytogenetics: X F1
• Gene Summary: E3 ubiquitin-protein ligase that catalyzes 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains formation. Functions as an inhibitor of cytokine gene transcription. Inhibits IL2 and IL4 transcription, thereby playing an important role in the induction of the anergic phenotype, a long-term stable state of T-lymphocyte unresponsiveness to antigenic stimulation associated with the blockade of interleukin production. Ubiquitinates ARPC5 with 'Lys-48' linkages and COR1A with 'Lys-63' linkages leading to their degradation, down-regulation of these cytosleletal components results in impaired lamellipodium formation and reduced accumulation of F-actin at the immunological synapse. Functions in the patterning of the dorsal ectoderm; sensitizes ectoderm to respond to neural-inducing signals (By similarity).[UniProtKB/Swiss-Prot Function]