Snrpn (NM_001082962) Mouse Untagged Clone

CAT#: MC201309

Snrpn (untagged) - Mouse small nuclear ribonucleoprotein N (Snrpn), transcript variant 3, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Snrpn
• Synonyms: 2410045I01Rik; HCERN3; Peg; Peg4; Pwc; sm-D; SMN; snRNP-N
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC019589 sequence for NM_001082962
  GGAGAGGGAGCCGGAGATGCCAGACGCTTGGTTCTGAGGAGTGATTTGCAACGCAATGGAGCGAGGAAGG GATCGCTTACACTTGAGAAGAACTACTGAACAGCACGTGCCCGAGGTCGAGGTCCAGGTCAAACGTCGAA GGACAGCCTCACTGAGCAACCAAGAGTGTCACTTGTACCCACGACGTTCTCAGCAACAGCAAGTTCCTGT GGTGGATTTCCAGGCAGAACTAAGACAGGCATTCTTAGCTGAGACACCAAGAGGTGGTTAAAGCAGTATT GCAACTTCAAGGTGGTGGAATTCAAGGAAAACAGGACCCATCCTCTGTCTGTGAAACTTTGGTCAAGCTT ACATTGTTTGTTAATAGCCTGCATCGAACCTTTATTTATATCCCTTCCCCGAGTATTAAGGATCTTGAAA TTTTAGTGTTGACAACGGCTATTGTGGAACAGCAATCATGACTGTGGGTAAGAGTAGCAAGATGCTGCAG CACATTGACTATAGGATGAGATGTATCCTGCAAGATGGGAGAATCTTCATTGGCACCTTCAAGGCTTTTG ACAAGCATATGAATTTGATCCTCTGTGATTGTGATGAGTTCAGGAAGATCAAGCCAAAGAATGCAAAACA GCCAGAACGTGAAGAAAAACGGGTTTTGGGTCTGGTCTTGCTACGTGGGGAGAACTTGGTTTCAATGACT GTGGAGGGCCCACCTCCTAAAGATACTGGCATTGCTCGTGTGCCTCTTGCTGGCGCTGCAGGTGGCCCTG GGGTTGGAAGAGCAGCTGGCAGAGGAGTGCCAGCAGGTGTACCTATTCCCCAGGCTCCTGCTGGATTAGC AGGCCCTGTCAGAGGAGTTGGAGGCCCATCCCAGCAGGTCATGACCCCACAGGGAAGAGGCACTGTTGCA GCTGCTGCTGTTGCTGCTACTGCTAGCATTGCAGGAGCCCCAACCCAGTACCCGCCAGGACGGGGAACTC CACCTCCACCTGTAGGCAGAGCAACCCCACCTCCAGGCATTATGGCTCCTCCACCTGGTATGAGACCACC CATGGGCCCACCCATTGGGCTTCCCCCTGCTCGTGGGACACCTATAGGCATGCCTCCTCCAGGAATGAGA CCCCCTCCACCAGGAATTAGAGGCCCACCTCCCCCAGGAATGCGCCCACCAAGACCCTAAGATACAGTTG ATAAATCTCAGCCCTTCTCTTCCCCTACAATGCTTCTTGTGAAATTGTGTACCTGCAAGCTTTTTGACCC CTCTTACTGCATTAACTATAGATAATAAATACATAGAGCAATTCAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_001082962
• Insert Size: 723 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC019589, AAH19589
• RefSeq Size: 1319 bp
• RefSeq ORF: 723 bp
• Locus ID: 20646
• UniProt ID: P63163
• Cytogenetics: 7 34.04 cM
• Gene Summary: This locus represents a paternally-expressed imprinted gene that encodes a component of the small nuclear ribonucleoprotein complex, which functions in pre-mRNA processing. Genomic and genetic changes in this region result in growth defects and lethality; the corresponding region in human is the critical region for Prader-Willi Syndrome. Alternative promoter use and alternative splicing result in a multitude of transcript variants encoding the same protein. Transcript variants may be bicistronic and also encode the SNRPN upstream reading frame protein (Snurf) from an upstream open reading frame. In addition, long spliced transcripts for small nucleolar RNA host gene 14 (Snhg14) may originate from the promoters at this locus and incorporate exons shared with this gene. [provided by RefSeq, Mar 2017]
  Transcript Variant: This variant (3) represents use of an alternate promoter compared to variant 1.