2610002M06Rik (NM_025921) Mouse Untagged Clone

CAT#: MC201281

2610002M06Rik (untagged) - Mouse RIKEN cDNA 2610002M06 gene (2610002M06Rik), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: 2610002M06Rik
• Synonyms: 8430408J07Rik; AI876413; Chmp1b2
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC016070 sequence for NM_025921
  CTCGAAACCCGCTACAACCGCCACCCTCCGCGCGCCCAAGCGACCCACAACTCGCGGGTCCAGCCCCTGT CCCGCGTGTTACCTGCCCCTCCCCCTCCTCCCGCCCGGTGAACTCGAGAGATCATGTCGAACATGGAGAA ACACTTATTTAACTTGAAGTTTGCTGCAAAAGAACTTAATAGGAATGCCAAGAAGTGTGATAAAGAAGAA AAAGCTGAAAAGGCCAAGATAAAAAAGGCCATTCAGAAGGGCAATACAGAAGTTGCAAGAATACATGCTG AAAATGCAATCCGTCAGAAAAACCAAGCAATCAATTTCTTGAGAATGAGTGCTAGGGTTGATGCTGTGGC AGCCAGAGTTCAAACTGCAGTAACAATGGGCAAGGTGACAAAATCCATGGCAGGTGTAGTTAAATCTATG GATGCTACATTGAGGAGTATGAACCTTGAGAAGATTTCTGCCTTAATGGACAAATTTGAACATCAGTTTG AAACATTGGATGTTCAGACACAGCAAATGGAAGATACAATGAGTAGCACTACTACCCTTACGACACCACA GAACCAAGTGGATATGCTGCTCCAGGAAATGGCAGATGAAGCTGGCCTCGATCTCAATATGGAACTACCA CAGGGTCAGACAGGGTCTGTTGGTGCAAGTGTTGCTTCTACAGAGCAGGATGAACTGTCACAGAGGCTTG CTCGTCTTCGTGATCAAGTGTAACTTAAATAACTGGTGCTTCTGTTCCTCCACATATTTCTGAGCCATCA TTTGTGTGTGTGTCTATAGATAAGGTGTATTCCAGTTACAAAAACATCATACATGACAGGATGTTTGGAC ATCATTTCTATAAGATGCTTTCTTTTGCCTTGTGTATTTCACAGTGCATTGCGAAATTCCAAGGAATTTC CACATTGGCAGATTTTATAGTTCTATATATTGTGTGTAAAAGTGTGAGGGACATATTTTTCTAGATGTCT TTGGCAAAGCTAGCATTAGTTTTCTGTATATTATACATCTTCCGCAAATATTAGAGCAAACATCTAGCAC CAAAGTAATGCTAAGTAAAGTAATTTTGTCAGAATAATGTATAAAGGCTTTGTTTTTACTTATTGAAAGT AACTTTCAATTATTGTCTGTGATGAATTTTGATTTTGTAAAAATAAAAACAGGAAAGCATGTTCATTTAA TATGTTCGCATAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_025921
• Insert Size: 600 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC016070, AAH16070
• RefSeq Size: 1220 bp
• RefSeq ORF: 600 bp
• Locus ID: 67028
• UniProt ID: Q9CQD4
• Cytogenetics: X D
• Gene Summary: Probable peripherally associated component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis. ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Involved in cytokinesis. Involved in recruiting VPS4A and/or VPS4B and SPAST to the midbody of dividing cells (By similarity).[UniProtKB/Swiss-Prot Function]