Mthfd2 (NM_008638) Mouse Untagged Clone

CAT#: MC201235

Mthfd2 (untagged) - Mouse methylenetetrahydrofolate dehydrogenase (NAD+ dependent), methenyltetrahydrofolate cyclohydrolase (Mthfd2), nuclear gene encoding mitochondrial protein, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mthfd2
• Synonyms: AW558851; NMDMC
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC019511 sequence for NM_008638
  CCGGGGTATAGCTTCGTGGCCCGCGCGCGCGCTTCTCTCCGGCGCGGGCACCGGCGCGAGCCATGGCTTC AGTTTCCTTGTTGTCTGCGTTGGCTGTGCGGTTGTTGCGCCCCACGCACGGCTGCCACCCCCGCCTACAG CCCTTCCACCTGGCGGCAGTGCGAAATGAAGCCGTTGTCATTTCTGGAAGGAAACTGGCCCAGCAGATCA AGCAAGAAGTACAGCAGGAGGTGGAAGAGTGGGTGGCCTCAGGCAATAAGCGGCCACACCTCAGTGTCAT TCTGGTTGGTGACAATCCCGCCAGTCACTCCTATGTTCTCAACAAAACCAGGGCAGCAGCTGAAGTGGGA ATCAACAGTGAGACAATTGTGAAACCAGCCTCAGTTTCAGAGGAAGAGCTGCTGAATTCAATCAGGAAGC TGAACAACGATGAGAACGTAGATGGCCTCCTTGTTCAGCTGCCACTCCCAGAGCACATTGATGAGAGGAA GGTCTGCAATGCCGTTTCTCCTGACAAGGATGTTGATGGCTTTCATGTCATTAACGTGGGGCGAATGTGC TTGGACCAGTACTCTATGCTGCCAGCAACCCCATGGGGCGTGTGGGAGATAATTAAGCGAACAGGCATTC CAACCTTAGGGAAGAATGTGGTAGTGGCTGGCAGGTCAAAAAACGTTGGAATGCCGATTGCAATGTTGCT GCACACAGATGGAGCTCACGAACGGCCCGGGGGTGATGCCACAGTCACAATATCTCATCGATACACTCCC AAAGAGCAGCTGAAGAAGCACACGATCCTTGCAGACATTGTAATATCTGCCGCTGGCATTCCAAATCTGA TCACAGCTGACATGATCAAGGAAGGAGCAGCGGTCATCGATGTGGGAATAAACAGAGTTCAAGATCCTGT CACTGCAAAGCCCAAATTGGTTGGAGATGTGGATTTTGAAGGAGTCAAGAAGAAAGCTGGTTATATCACT CCCGTCCCTGGTGGTGTGGGTCCCATGACCGTGGCCATGCTCATGAAGAACACCATTATTGCCGCAAAGA AAGTGCTAAGACCAGAGGAGCTGGAAGTGTTCAAGTCCAAACAGCGCGGAGTCGCCACCAACTAGCAGCA CTCTGAGCCAGCCCGAGAGGCTAAGCAGGCCAAGAGGAATGCAGTGTTTTTAATTTATTCTGTCGAAATG GTTTAAAATGTTTGTATTTATTGAAAGCTTAAACGGGTGGCTGTGTGCGCGCATGGCTCTACAGAACCTC ACCAGGATGCCCATCAGTTGTCCTGCCTTTGACCTGGTGATGCACAGGAGACGTCCTCACACCGAGAGTG GATGCTGAACTTCATCAAAAAAAAAAAAAGGTCTGCCGTTTTAGGCTCTCATTTCCCAAGTGCTATTCCA ATACAAGTTGATGACTCATCTTAGGTCCCAAGCCTTTGAGTTCAACTTTCAAACCAAAGGAAACCTTAGT AGAGGAAATGAAGGGAAAGGAAGAGAAAGCAGCACAGGGTTTCAACGGCACTCCCTTACTCTCTCGGGAG TGCAGCCAGGAGTGCTCCGATTGCCCTGAGGAGAGCTCAGCTGGCTGGGCCTAGCTGTCCCCCTGTGGCG GGTCGTCAGGGAAGGGTTAGAGTCTCCCCACTTGCTGTTATCCTCCTGTGAGCTTGTCCTATCTATTTTA ATTTTCTAGAATCGAAAAAGCTCATTTTATAAATGATGTGTGTATTATCACGTTTGGCTTTTGCAGCACA TTTAAACTTTTAAGTTTCAGACTGTTAAGAGAGTTGTGTGTCTGTTTTCTTTGGCGGGTGCTTTGAGGCC TATTTGTTTTTGAAAAACAAAACAAAACTTGTGCGTGATAATCACGAGGGCAGCTCGTGCCACCAAAGAA CTGTCAGCAGCTGCCTGCACGTCTTTCACGTACCCCGTTGGCTTTCCTGTAAGCTATCCAGGAGCTTGGG TTAATATAAGACTTAGTCGGATTTTCTTATTAAAGGGTTTTCTTGCAGTTGGGTTTTTTGTTTGTTTTTA AATAAAGCTCATCTGTCGGGTGTGGAATGCGTTTCTGAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_008638
• Insert Size: 1053 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC019511, AAH19511
• RefSeq Size: 2083 bp
• RefSeq ORF: 1053 bp
• Locus ID: 17768
• UniProt ID: P18155
• Cytogenetics: 6 35.94 cM
• Gene Summary: Although its dehydrogenase activity is NAD-specific, it can also utilize NADP at a reduced efficiency.[UniProtKB/Swiss-Prot Function]