Ehhadh (NM_023737) Mouse Untagged Clone

CAT#: MC201157

Ehhadh (untagged) - Mouse enoyl-Coenzyme A, hydratase/3-hydroxyacyl Coenzyme A dehydrogenase (Ehhadh), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ehhadh
• Synonyms: 1300002P22Rik; HD; L-PBE; LBFP; LBP; MFP; MFP1; PBFE
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC016899 sequence for NM_023737
  CCCACGCGTCCGAGGATACCTTGAGAAAATGGCTGAGTATCTGAGGCTGCCCCACTCCCTGGCTATGATC CGCCTCTGCAATCCACCGGTCAATGCCATCAGTCCAACTGTAATCACAGAAGTAAGGAATGGACTCCAGA AAGCTAGTTTGGACCATACGGTTAGAGCCATAGTGATCTGTGGAGCAAATGACAACTTCTGTGCAGGTGC TGATATCCATGGCTTTAAATCTCCCACTGGCCTTACATTAGGAAGCTTGGTAGATGAAATACAGCGATAC CAGAAGCCAGTGGTGGCCGCCATCCAAGGCGTGGCTCTTGGAGGAGGACTAGAGCTGGCCTTGGGCTGTC ACTATCGGATTGCCAATGCAAAGGCTCGTGTTGGCTTCCCGGAAGTGATGCTGGGAATTCTTCCTGGTGC AAGAGGAACGCAGCTTCTACCCAGGGTCGTTGGAGTTCCTGTTGCTCTTGACTTAATTACCTCAGGAAGA CATATTTCAACAGATGAAGCACTCAAGCTTGGAATTCTGGATGTAGTTGTAAAGTCAGACCCAGTTGAAG AAGCCATCAAATTTGCTCAGACGGTTATAGGTAAACCCATAGAACCCCGCAGGATCCTAAACAAGCCAGT CCCAAGCTTGCCCAACATGGACAGTGTTTTTGCAGAAGCCATTGCCAAGGTACGGAAGCAGTACCCTGGC CGCCTGGCTCCGGAGACTTGTGTCCGTTCAGTCCAGGCCTCCGTGAAGCATCCATATGAAGTGGCCATCA AGGAAGAAGCAAAGCTGTTTATGTACCTTCGGGGGTCGGGGCAGGCTAGAGCCCTGCAGTACGCCTTTTT TGCTGAAAAGTCTGCAAATAAGTGGTCAACTCCCTCAGGAGCATCTTGGAAAACAGCATCTGCTCAACCC GTCTCCTCGGTTGGTGTTCTTGGCTTGGGAACGATGGGCCGAGGCATCGCCATCTCTTTTGCAAGGGTGG GGATCCCTGTGGTTGCTGTAGAGTCAGACCCAAAGCAGCTAGATACTGCAAAGAAGATAATAACTTCCAC CTTGGAAAAGGAAGCATCGAAGAGTGGCCAAGCTTCAGCAAAACCAAACCTCAGGTTCTCCTCATCCACA AAGGAGCTTTCGAGTGTGGATTTAGTGATTGAAGCAGTGTTCGAAGATATGAACCTGAAGAAGAAGGTCT TCGCTGAACTGTCAGCCCTGTGCAAGCCGGGAGCCTTTCTGTGCACCAATACCTCAGCACTGGATGTGGA TGACATTGCTTCTTCCACAGATCGCCCCCAGCTGGTGATTGGCACCCACTTCTTCTCCCCAGCCCACATC ATGAGGTTACTAGAGGTCATTCCTAGCCGATACTCTTCCCCCACTACCATCGCCACAGTCATGAGCTTAT CCAAAAGGATTGGAAAGATTGGAGTCGTTGTTGGTAACTGCTATGGATTTGTTGGGAATCGAATGTTGGC TCCCTATTACAACCAGGGCTATTTCTTGATAGAGGAAGGTAGTAAGCCAGAGGATGTAGATGGGGTCTTA GAAGAGTTTGGTTTTAGAATGGGACCCTTCAGGGTGTCTGACCTCGCAGGGCTAGATGTGGGTTGGAAAG TTCGCAAAGGGCAAGGCCTTACTGGACCGTCCTTACCTCCAGGAACCCCCACCCGAAAGAGGGGCAATAC CAGGTACTCCCCAATTGCTGATATGCTCTGTGAAGCTGGGCGATTTGGTCAGAAGACAGGTAAGGGCTGG TATCAGTATGACAAGCCACTGGGTCGCATCCACAAACCTGATCCCTGGCTTTCTGAGTTTCTGTCACAGT ATAGAGAAACCCATCACATCAAGCAGCGCTCCATCAGCAAGGAGGAAATCCTGGAGCGTTGCTTATATTC CCTTATCAACGAGGCATTCCGCATCTTGGAGGAGGGGATGGCCGCTAGCCCAGAGCACATTGATGTCATC TACTTGCATGGGTATGGGTGGCCAAGGCACGTGGGTGGGCCCATGTACTATGCTGCCTCAGTTGGGCTGC CCACAGTTCTAGAGAAATTGCAGAAATATTACAGACAGAATCCTGACATCCCCCAGCTGGAGCCCAGTGA CTACCTGAGGAGGCTGGTTGCCCAGGGAAGCCCTCCTCTGAAAGAATGGCAAAGCTTGGCAGGACCCCAT AGCAGCAAACTGTGATTCAGCCTTCTGCATTTTGCCATGTATGGTGGCATCTAATTTCAGTGTGATTCAT TCCTAAAATCCAAACAGATTTCTCTGAAGTACAAATGATGATTGCTGCTGTTGCTGCTGCTGCTGGTGGT GGTGGTGCTAAATGGTCAGCAAAAGCCCCTCTCTGTATCTTCCTACCTCATAATTCTAATGGCCAGATTT CAGGAATGTGTTTTCTATACCTCTGACGGTACTTCTATCAGAAAAATTCAGCAAATATGTATTTACTACT GCAAGCGTTCTTCTCTTTTGACTGTTTGCTTGGTATAGCCATAACTTATACAAAATGTTGCTAAGAGAAT AATTCATAACTCCAGTTGACAACCCAGATATACCTTTTAGCAAATCAACAAAAGTGGCATGGACATACAA GTCACACATTTCAGCTGTGTGACGGAACATGATGCACCATGGTGCTGTGTTGCAGCATTGCTCTGACTAA GAGGAGGCAGCCAAAGAAGGCCACATGTCATATGGATGTGGATATAAAATTCCAGAATATACAGATCTGA GAACAATGTAGATGAGTAGCCCAAGGCTATAATTGTTTTAAGTTAAAATTACTGCTGATTAATACAGAGT ATCTTTGGAGTTTGATAAACTTTTCTAAAATTACTTTAGTGGTGTATCTGTAACACATTGAGTTCAATGG TTCAAAGTGAACTATGTAGTGTGTGTGTATATACACACAACATATATATATATACATATATATATATATA TTCTATCTCAATGAAGTTAATAAAATTAATGATGGTAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_023737
• Insert Size: 2157 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC016899, AAH16899
• RefSeq Size: 2993 bp
• RefSeq ORF: 2157 bp
• Locus ID: 74147
• UniProt ID: Q9DBM2
• Cytogenetics: 16 B1
• Gene Summary: Peroxisomal trifunctional enzyme possessing 2-enoyl-CoA hydratase, 3-hydroxyacyl-CoA dehydrogenase, and delta 3, delta 2-enoyl-CoA isomerase activities. Catalyzes two of the four reactions of the long straight chain fatty acids peroxisomal beta-oxidation pathway. Optimal isomerase for 2,5 double bonds into 3,5 form isomerization in a range of enoyl-CoA species. Also able to isomerize both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species (By similarity). With HSD17B4, catalyzes the hydration of trans-2-enoyl-CoA and the dehydrogenation of 3-hydroxyacyl-CoA, but with opposite chiral specificity (Probable). Regulates the amount of medium-chain dicarboxylic fatty acids which are essential regulators of all fatty acid oxidation pathways (PubMed:24075987). Also involved in the degradation of long-chain dicarboxylic acids through peroxisomal beta-oxidation (By similarity).[UniProtKB/Swiss-Prot Function]