Tcea3 (NM_011542) Mouse Untagged Clone

CAT#: MC201153

Tcea3 (untagged) - Mouse transcription elongation factor A (SII), 3 (Tcea3), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tcea3
• Synonyms: S-II; SII-K1
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC010807 sequence for NM_011542
  CGGACGCGTGGGTCGACGTGCGGCTCGCGCCTTGGCGCCCGCTGCTGCCCTGCGCCCCTAACCCCGCCGT GCGTTGTGGACCAACATGGGCCTGGAAGAGGAGCTGTTGAGGATCGCCAAAAAGCTGGAGAAGATGGTGT CCCGGAAGAAGACGGAAGGGGCCCTGGACCTTCTGAAGAAGCTGAACAGTTGCCAGATGTCCATCCAGCT GCTACAGACCACCAGGATCGGAGTGGCAGTCAACGGAGTCCGCAAGCACTGCTCAGACAAGGAAGTGGTG TCCTTGGCCAAAGTCCTCATTAAAAACTGGAAGCGGCTGCTGGACTCCCCCAGAACCACCAAAGGAGAAA GAGAAGAAAGAGAAAAAGCGAAGAAGGAGAAAGGACTTGGCTGTTCAGACTGGAAACCAGAAGCAGGCCT TTCTCCACCAAGGAAGAAAGGAGGGGGAGAGCCCAAAACTAGGAGAGACTCTGTAGACTCCAGGTCATCC ACCACCTCCTCTCCAAAAAGGCCTTCGCTGGAAAGATCCAACAGCAGCAAATCTAAAGTGGAAACTCCCA CGACCCCCAGCAGCCCCTCAACTCCCACCTTTGCCCCCGCTGTCTGTCTCCTGGCCCCCTGCTACCTCAC CGGGGACTCTGTCCGGGACAAATGTGTGGAGATGCTGTCAGCAGCTCTGAAGGCAGAAGACAATTTCAAG GACTATGGAGTCAACTGTGACAAGCTGGCCTCAGAAATAGAAGACCATATCTATCAAGAACTCAAGAGCA CAGACATGAAGTACCGGAACCGTGTGCGCAGCCGCATCAGCAACCTGAAGGACCCCAGGAACCCAGGCCT GAGGCGGAATGTGCTCAGTGGGGCCATCTCCCCAGAGCTCATTGCCAAGATGACAGCGGAGGAAATGGCC AGTGATGAGCTGAGGGAGCTGAGGAACGCCATGACTCAAGAGGCCATCCGTGAGCACCAGATGGCGAAGA CAGGCGGCACCACCACTGACCTCTTGCGGTGTAGCAAGTGCAAGAAGAAGAATTGTACCTATAACCAGGT GCAGACACGCAGTGCTGATGAGCCCATGACCACTTTTGTCTTATGCAATGAATGTGGGAATCGCTGGAAG TTCTGCTGACGGAACCACCCACCAGGATGGCAGAGGATAAAGTGAAGAAGACCCAGCAGGAAGCACAGAC CACGTGACCCAGAGCAAAAAAAAATAAAAAATACAAATTCAAATGACAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_011542
• Insert Size: 1044 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC010807, AAH10807
• RefSeq Size: 1252 bp
• RefSeq ORF: 1044 bp
• Locus ID: 21401
• UniProt ID: P23881
• Cytogenetics: 4 D3
• Gene Summary: Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus.[UniProtKB/Swiss-Prot Function]