Erlin1 (BC011220) Mouse Untagged Clone

CAT#: MC201116

Erlin1 (untagged) - Mouse ER lipid raft associated 1 (cDNA clone MGC:19110 IMAGE:4208238), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Erlin1
• Synonyms: 2810439N09Rik; C80197; Keo4; Spfh1
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC011220
  CAGTTGGCGGGGCCGGGTGGCGGCTGCACGTGCGGGCGGGGGCGATGCGTCACTGACCGGAGGAACAAGG ATGAATATGACTCAAGCCCGGCTTCTGGTGGCTGCAGTGGTGGGGTTGGTGGCGATCCTCCTGTACGCCT CCATCCACAAGATCGAAGAGGGACACTTGGCCGTGTACTACAGGGGAGGAGCTTTGCTAACGAGCCCCAG TGGACCAGGCTATCATATCATGTTGCCTTTCATTACAACATTCAGATCTGTGCAGACAACACTACAAACG GATGAAGTTAAAAATGTGCCTTGTGGAACAAGTGGTGGAGTCATGATCTATATTGACCGAATAGAAGTGG TTAATATGTTGGCTCCTTATGCAGTGTTTGACATTGTGAGGAACTATACTGCAGACTACGACAAGACTTT AATCTTCAATAAAATCCACCATGAGCTGAACCAGTTTTGCAGTGCCCACACACTTCAAGAAGTTTACATA GAATTGTTTGATCAAATAGATGAAAACCTGAAGCAGGCCCTGCAAAAAGATTTAAACACCATGGCCCCAG GTCTCACTATCCAGGCTGTGCGTGTTACAAAACCCAAAATCCCAGAAGCCATAAGAAGAAATTTTGAATT AATGGAGGCAGAGAAGACAAAACTTCTCATAGCTGCACAGAAACAAAAGGTGGTGGAGAAAGAAGCTGAG ACGGAGAGGAAAAGGGCTGTTATAGAAGCAGAGAAGATTGCACAAGTAGCAAAAATTCGATTTCAACAGA AAGTGATGGAGAAAGAAACTGAAAAACGCATTTCTGAGATTGAAGATGCTGCGTTCCTGGCCCGAGAGAA GGCGAAAGCAGATGCCGAGTATTACGCTGCACACAAATACGCCACCTCAAACAAGCACAAACTGACCCCA GAGTATCTGGAGCTCAAGAAATACCAGGCCATTGCCTCAAACAGTAAGATCTACTTTGGCAGCAACATCC CCAGCATGTTTGTGGACTCCTCCTGTGCTCTGAAATACTCTGATGGTAGGACTGGGAGAGAAGACTCCCT TCCCCCAGAGGAGGCCCGTGAGCCCTCTGGAGAGAGCCCCATCCAAAACAAGGAGAACGCAGGTTGATGC AAGAGGTGGAAATGTTCTCCCATATCAAGATGCGACCCAAGGGGCTAAGTGGGAACAGTGGTTATGTGGA CTCGTAAGATTCACAGAGAATGTGTGCTCTGTTGTGATTCTCTTGTCATAGTCCTGGTTTGCCAGCTGAC TACAGGATAGACCCAGCTGTCTGGCACTCAAACGGTCTCTGCAGCCACAGTTTTATCAAGTATCCTGTAT GTGTTCCTTTGTAAACCGGTACTCATGAATGAGGGAAAGTCTGATGCTAAGATACTGCCTGCACTGGAAT GTCAAACACTATATAACAAGCTGTGGTTTTTAAAAGCTATTGAATAATGTTTACATTGGTCCCTGAGGAC ATGTGTGCTCAGACATTCAAGAGCTAGGAGGCCAGAGAGAAGACCTTCAGAAAACGGTAAGTTAAAGAAG ACAAGTGTCATCAGACACTTGGGACCCGGGCTCTCTTTAAAGTCTAGTCCCGGCATTCCTCCATGTGATT GACAGCCAGACCTCTGGGTTCCCAGGAAATTATCTTCCAGTCGAATGACCATTTACTTGATACAAATTGT ACCTTTCTGTTTTTCTAGTCAGGTTGGTGGCCTGCAGGGACGCGTACTTTGCCACCCGACCAGAGGTTCC TCGAAGATATTCCCAATCACTAGTTTATTGCGTTAGGAGACTCAGAGATATAGAAAGCAGCTGAAATTTA AGGGAGATAAAGCCTGCACTGCACCAAAGCTACGGGTCCCTGTGTTTCCTCTATTCAGTGATGTCATCAA CCTCACTGTCCCAGCCCATGTGTGACTAAAGTGCCCGGTTTTAGCCACAGACAACTGCTTAGATGTCACC TCTTGGCTGACCAAAGCTGGGACAGGGCTTTAACCAGACATAGGAGCAGTGTGCAATTCCTGATTCACTG CACAGTATTATGTCATAATTGCAGGAATTATTTTTTGTTTTTAAAACTGGATTTGGGGCACATTCATTCA CCCCAACACTTCTATCTAAAGGCCAAGGTTCTAGGGCTGCTATGGTCACTAACACACTGATTCTCCTTAA AGTAATTCTCGAAGTGTGGAACAAAGTGACCGAGACAGCATCCTCAGTCATCTTTGTCTCCTTCCCTGGG ATGCAGATACCGAAGTTGCTTTTCCAACTTTCGCCTCCGCTAGGAGATCAGAAAGAATTCTTGTGACTTC CTGGGCAGCCATTGAATTCATTTTCCATGAGAAGATGACAGAGTTAGCCTGTGGCTATAGGAGATCATGT CATCCAGACCTTTTTGCCCATCACATTAACTTTCCTGGAATATTGTGCTGCACAGGTAGACCTGAGTCTG CCCAGCTTGTTGACAGCTCTTGTGTATACTGTGTTGAAGCCAGACAGAAAAGTAATGGGGCCACTTCTGA AACCTCTCAGCTGTTGATCTCACAGCAGCTAAAGGGTTGTGCCAAACATTTTATTAAGAAAGTAAAGCCC AGATTTGAATGGGGGTTTTCCCTAGGCCTTATAGTATAGAGGCATTTGTAATATGGAGAAAATAATTTTT CTCATTTAATTATAGAAATTACCTTCAAACAGATTTTGTGTTCTTTGGCCCTTCAAATACTGGTGTTACA TTGTTGCTGCAGATAAATGATGATTGTCGTGGGATATCTGGATCACTGAGCTCTGTGCTTTCATTCCTAG AGATGTTTCTCATTCCCATTTAGTGAAATGCTGTTGCCCCAAAGTGATGGTTGTGGGATTTCTTACCGGT CATAGGCCCCGGTGAGGAGCAGGGAAGCGCCATTGTGAAAGATTAAAGAAAGCACTTCCACTTGAGCTCC TTATGGAGTGAGCTTCCCTGTGCCCACTCAGTGAACTAAGTCTGACCATCCTTCAGGGACGTTCCTTTTG GTAAATATACACTGTAATCTTTAAGTCTAAATTTATATGTGAAAGTTAACTTTTTTTAAAAACCTAAATA AAATTATTTTCCTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: BC011220
• Insert Size: 1041 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC011220, AAH11220
• RefSeq Size: 3127 bp
• RefSeq ORF: 1041 bp
• Locus ID: 226144
• Cytogenetics: 19 C3
• Gene Summary: Component of the ERLIN1/ERLIN2 complex which mediates the endoplasmic reticulum-associated degradation (ERAD) of inositol 1,4,5-trisphosphate receptors (IP3Rs). Involved in regulation of cellular cholesterol homeostasis by regulation the SREBP signaling pathway. Binds cholesterol and may promote ER retention of the SCAP-SREBF complex (By similarity).[UniProtKB/Swiss-Prot Function]