Cyp2a4 (BC010761) Mouse Untagged Clone

CAT#: MC201111

Cyp2a4 (untagged) - Mouse cytochrome P450, family 2, subfamily a, polypeptide 4 (cDNA clone MGC:18511 IMAGE:4239813), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cyp2a4
• Synonyms: AI893559; Cyp15a1; D7Ucla4
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC010761
  ATCACCCCACTGCCACCATGCTGACCTCAGGACTCCTCCTGGTGGCTGCAGTGGCCTTCCTCAGCGTCCT GGTTTTGATGTCTGTCTGGAAGCAGAGAAAGCTCTCAGGAAAGCTGCCTCCAGGACCCACCCCACTGCCC TTCGTTGGGAACTTCCTTCAGCTGAACACAGAGCAAATGTACAACTCTCTCATGAAGATCAGCCAACGTT ATGGTCCTGTATTCACCATCTATCTGGGATCTCGCCGAATTGTGGTGCTGTGCGGACAGGAGACAGTCAA GGAAGCTCTGGTGGACCAAGCTGAGGAATTCAGCGGGCGGGGCGAGCAGGCTACCTTCGACTGGCTTTTC AAAGGCTATGGCATAGCCTTCAGCAGCGGGGAGCGAGCCAAACAGCTAAGGCGCTTCTCCATCACCACGC TGCGGGACTTCGGCGTGGGGAAGCGTGGCATCGAGGAGCGCATCCAAGAGGAGGCGGGCTTTCTCATCGA TTCATTTCGGAAGACGAACGGTGCTTTCATTGACCCCACCTTCTACCTTAGCCGAACAGTCTCCAATGTC ATTAGCTCAATCGTCTTCGGGGACCGCTTTGACTATGAGGACAAAGAGTTCCTGTCACTTCTTAGAATGA TGCTGGGAAGCCTCCAGTTCACTGCTACCTCCATGGGGCAGGTCTATGAGATGTTTTCTTCTGTGATGAA ACACCTGCCAGGGCCCCAGCAACAGGCCTTTAAGGAGCTGCAAGGCCTGGAGGACTTTATAACCAAGAAA GTGGAACACAATCAGCGCACGCTGGATCCCAATTCCCCAAGGGACTTCATCGACTCCTTCCTCATCCGAA TGCTGGAGGAGAAGAAGAACCCCAATACTGAGTTCTACATGAAGAACTTGGTGCTGACTACACTAAATCT CTTCTTTGCTGGCACAGAGACCGTCAGCACCACCCTGCGCTATGGCTTTCTGTTGCTCATGAAGTACCCA GATATTGAGGCCAAGGTCCATGAGGAGATTGATCGGGTGATTGGCAGGAACCGGCAGCCCAAGTATGAGG ACCGAATGAAGATGCCCTATACGGAGGCTGTAATCCATGAGATCCAGAGATTTGCAGACCTGATCCCCAT GGGCCTGGCTCGAAGGGTCACCAAGGACACCAAGTTTCGAGATTTCCTCCTCCCCAAGGGTACTGAAGTA TTTCCTATGCTGGGCTCTGTGCTGAAAGACCCCAAGTTCTTCTCCAACCCCAAAGACTTCAACCCAAAGC ACTTCCTAGATGACAAGGGACAGTTTAAGAAGAGTGATGCCTTTGTGCCCTTTTCCATTGGAAAACGGTA TTGTTTCGGAGAAGGACTGGCTAGGATGGAACTCTTCCTCTTCCTCACAAACATCATGCAGAACTTCCAC TTCAAATCCACACAGGAACCCCAGGACATCGATGTGTCTCCTAGACTCGTGGGCTTTGTCACGATCCCAC CAACCTACACTATGAGTTTCTTGTCCCGTTGATCCTGGGCTGCATGAGGTTAAAGGGAATGATTGAGACC AGACAAGTCAGGGGTTGAAACTTAGAAAAGGTCAAAGGTACAGAAGAAACAGAGGACACTTCGTAGACTT GCAGAGGATATTTCAAAGGTAGCCAGAGAAGGGGGAAATTATACTATGTTGTCAATAGGAATAATAAAAT AATAAAAGTAGATATTATTTATGGCAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: BC010761
• Insert Size: 1485 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC010761, AAH10761
• RefSeq Size: 1722 bp
• RefSeq ORF: 1485 bp
• Locus ID: 13086
• Cytogenetics: 7 14.39 cM
• Gene Summary: Highly active in the 15-alpha-hydroxylation of testosterone. Also active in the 15-alpha-hydroxylation of progesterone and androstenedione. Little or no activity on corticosterone, pregnenolone, dehydroepiandrosterone, estradiol or estriol.[UniProtKB/Swiss-Prot Function]