Acot7 (NM_133348) Mouse Untagged Clone

CAT#: MC201104

Acot7 (untagged) - Mouse acyl-CoA thioesterase 7 (Acot7), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Acot7
• Synonyms: 2410041A17Rik; Ach1; Act; Bach; Cte-II; CTE-IIa; Lach1
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC013507 sequence for NM_133348
  GCCTGCTGGACACCTGTTCTCAGATTCCGCCGCCGCCGCCGTCGTCCGCCGTCGCAGCCAAGATGTCCGG CCCCACCACAGACACGCCGGCCGCCATCCAGATCTGCCGGATCATGCGTCCAGATGATGCCAATGTGGCT GGCAATGTTCATGGAGGGACCATTCTGAAGATGATTGAGGAGGCCGGGGCCATCATCAGCACGCGGCACT GTAACAGCCAGAATGGGGAGCGCTGTGTGGCTGCCCTGGCACGGGTGGAGCGCACTGACTTCCTGTCACC CATGTGCATCGGCGAGGTGGCTCATGTCAGTGCAGAGATCACCTACACTTCCAAGCACTCTGTGGAGGTC CAGGTCCACGTGATGTCGGAGAACATCCTCACAGGTACCAAAAAGCTGACCAATAAAGCCACCTTGTGGT ATGTGCCCCTGTCATTGAAGAATGTGGACAAGGTCCTTGAGGTGCCTCCCATTGTGTATTTACGGCAGGA GCAGGAGGAGGAGGGTCGGAAACGCTATGAAGCCCAGAAGCTGGAACGCATGGAGACCAAGTGGAGGAAC GGAGACATTGTCCAGCCTGTCCTGAACCCAGAGCCGAACACGGTGAGCTACAGCCAGTCCAGCCTGATCC ACCTGGTGGGGCCCTCGGACTGCACCCTTCATGGCTTCGTGCACGGAGGTGTCACCATGAAGCTCATGGA TGAGGTGGCTGGGATTGTGGCTGCACGCCACTGCAAGACCAACATAGTAACTGCCTCTGTGGATGCCATC AATTTCCACGACAAGATCCGGAAAGGCTGTGTCATCACCATCTCCGGACGCATGACCTTCACAAGCAATA AGTCCATGGAGATTGAGGTCCTGGTGGACGCTGACCCTGTGGTGGACAACTCACAAAAGCGCTACCGGGC CGCCAGTGCCTTCTTCACCTACGTGTCCCTGAACCAGGAGGGCAAGCCAATGCCTGTGCCTCAGCTTGTG CCAGAGACGGAGGATGAGAAGAAGCGCTTCGAAGAAGGCAAAGGCCGTTATCTGCAGATGAAGGCGAAGC GACAGGGCCACACAGAGCCTCAGCCCTAGGTGTCTTCCTCCTGTCCCGGGTCAGCACAGTTGTGGCAATA GCCCAGTATGCAGTCACTTAGAAATTGCCCCCTTGGCCAAACCCCCGATTTCCACTGAGAGCTGGTGTTG TGTGAAGTGTTGAGTGGCAGTGTTCCCTATGGCCCATCCCCAAAACCTGTGCACCAAAGCTTTATTTATG TCCCCAGTGTTGTCCCAAAGGCCACCATGGACACCAGAGCACACCGACTGGCCTGAAGAAGCCAGCATCA CTAATAAAGCTGCTGTCTGGCTGGAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_133348
• Insert Size: 1017 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC013507, AAH13507
• RefSeq Size: 1369 bp
• RefSeq ORF: 1017 bp
• Locus ID: 70025
• UniProt ID: Q91V12
• Cytogenetics: 4 E2
• Gene Summary: Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH (PubMed:15288813). Acyl-coenzyme A thioesterase 7/ACOT7 preferentially hydrolyzes palmitoyl-CoA, but has a broad specificity acting on other fatty acyl-CoAs with chain-lengths of C8-C18 (Probable). May play an important physiological function in brain (PubMed:15288813).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR and 5' coding region, compared to variant 1, resulting in an isoform (2) with a distinct and shorter N-terminus, compared to isoform 1.