Hexa (NM_010421) Mouse Untagged Clone

CAT#: MC201099

Hexa (untagged) - Mouse hexosaminidase A (Hexa), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Hexa
• Synonyms: Hex-1
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC010755 sequence for NM_010421
  CTGGAAGGGGAGCTGGCCGGTGGGCCATGGCCGGCTGCAGGCTCTGGGTTTCGCTGCTGCTGGCGGCGGC GTTGGCTTGCTTGGCCACGGCACTGTGGCCGTGGCCCCAGTACATCCAAACCTACCACCGGCGCTACACC CTGTACCCCAACAACTTCCAGTTCCGGTACCATGTCAGTTCGGCCGCGCAGGCGGGCTGCGTCGTCCTCG ACGAGGCCTTTCGACGCTACCGTAACCTGCTCTTCGGTTCCGGCTCTTGGCCCCGACCCAGCTTCTCAAA TAAACAGCAAACGTTGGGGAAGAACATTCTGGTGGTCTCCGTCGTCACAGCTGAATGTAATGAATTTCCT AATTTGGAGTCGGTAGAAAATTACACCCTAACCATTAATGATGACCAGTGTTTACTCGCCTCTGAGACTG TCTGGGGCGCTCTCCGAGGTCTGGAGACTTTCAGTCAGCTTGTTTGGAAATCAGCTGAGGGCACGTTCTT TATCAACAAGACAAAGATTAAAGACTTTCCTCGATTCCCTCACCGGGGCGTACTGCTGGATACATCTCGC CATTACCTGCCATTGTCTAGCATCCTGGATACACTGGATGTCATGGCATACAATAAATTCAACGTGTTCC ACTGGCACTTGGTGGACGACTCTTCCTTCCCATATGAGAGCTTCACTTTCCCAGAGCTCACCAGAAAGGG GTCCTTCAACCCTGTCACTCACATCTACACAGCACAGGATGTGAAGGAGGTCATTGAATACGCAAGGCTT CGGGGTATCCGTGTGCTGGCAGAATTTGACACTCCTGGCCACACTTTGTCCTGGGGGCCAGGTGCCCCTG GGTTATTAACACCTTGCTACTCTGGGTCTCATCTCTCTGGCACATTTGGACCGGTGAACCCCAGTCTCAA CAGCACCTATGACTTCATGAGCACACTCTTCCTGGAGATCAGCTCAGTCTTCCCGGACTTTTATCTCCAC CTGGGAGGGGATGAAGTCGACTTCACCTGCTGGAAGTCCAACCCCAACATCCAGGCCTTCATGAAGAAAA AGGGCTTTACTGACTTCAAGCAGCTGGAGTCCTTCTACATCCAGACGCTGCTGGACATCGTCTCTGATTA TGACAAGGGCTATGTGGTGTGGCAGGAGGTATTTGATAATAAAGTGAAGGTTCGGCCAGATACAATCATA CAGGTGTGGCGGGAAGAAATGCCAGTAGAGTACATGTTGGAGATGCAAGATATCACCAGGGCTGGCTTCC GGGCCCTGCTGTCTGCTCCCTGGTACCTGAACCGTGTAAAGTATGGCCCTGACTGGAAGGACATGTACAA AGTGGAGCCCCTGGCGTTTCATGGTACGCCTGAACAGAAGGCTCTGGTCATTGGAGGGGAGGCCTGTATG TGGGGAGAGTATGTGGACAGCACCAACCTGGTCCCCAGACTCTGGCCCAGAGCGGGTGCCGTCGCTGAGA GACTGTGGAGCAGTAACCTGACAACTAATATAGACTTTGCCTTTAAACGTTTGTCGCATTTCCGTTGTGA GCTGGTGAGGAGAGGAATCCAGGCCCAGCCCATCAGTGTAGGCTACTGTGAGCAGGAGTTTGAGCAGACT TGAGCCACCAGTGCTGAACACCCAGGAGGTTGCTGTCCTTTGAGTCAGCTGCGCTGAGCACCCAGGAGGG TGCTGGCCTTAAGAGAGCAGGTCCCGGGGCAGGGCTAATCTTTCACTGCCTCCCGGCCAGGGGAGAGCAC CCCTTGCCCGTGTGCCCCTGTGACTACAGAGAAGGAGGCTGGTGCTGGCACTGGTGTTCAATAAAGATCT ATGTGGCATTTTCTCTAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_010421
• Insert Size: 1587 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC010755, AAH10755
• RefSeq Size: 1858 bp
• RefSeq ORF: 1587 bp
• Locus ID: 15211
• UniProt ID: P29416
• Cytogenetics: 9 32.02 cM
• Gene Summary: This gene encodes a member of the glycosyl hydrolase 20 family of proteins. The encoded preproprotein is proteolytically processed to generate the alpha subunit of the lysosomal enzyme beta-hexosaminidase. This enzyme, together with the cofactor GM2 activator protein, catalyzes the degradation of the ganglioside GM2, and other molecules containing terminal N-acetyl hexosamines. Mice lacking the encoded protein exhibit accumulation of gangliosides in the brain and membranous cytoplasmic bodies in neurons. Certain mutations in the human ortholog of this gene cause Tay-Sachs disease. [provided by RefSeq, Aug 2016]