Kcnj15 (BC010794) Mouse Untagged Clone

CAT#: MC201073

Kcnj15 (untagged) - Mouse potassium inwardly-rectifying channel, subfamily J, member 15 (cDNA clone MGC:18857 IMAGE:4221516), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Kcnj15
• Synonyms: IRKK, Kir4.2
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC010794
  GGAGAACTGGGGAGTCCTTGCCATTCCAAGCCGCCCTGTGCCGGAGCCTGCACTGGGCACTCGGCAGGTA AGAAGCTGAAGTGTCTGTCACTGTGGGGGCACGCAGCATGCTGGAGATGCTTCTGAGAAACTTGACTTCC AAAGGCCATAGCAGAGCCCCATGGTAGCCAGGTGGGAGAAGGGGAGCGAGGACGCGCCACTTACCCTGCA GAAGATTCCTGACCTACAGAGTGAGTGACCAGGTGGTCCAAAGATGGATGCCATTCACCTTGGCATGTCC AGTGCCCCACTGGTGAAGCATACCAACGGGGTTGGACTCAAGGCCCACAGACCCCGAGTCATGTCAAAGA GTGGGCACAGTAATGTGAGAATCGATAAGGTAGACGGAATCTATTTACTCTACCTCCAGGACTTGTGGAC AACCGTCATCGACATGAAGTGGCGATACAAGCTCACCCTATTTGCTGCCACCTTTGTGATGACCTGGTTT CTGTTTGGAGTGGTCTACTATGCCATAGCCTTTATTCATGGTGACTTACAACTTGGGGAATCTAATTCCA ACCACACACCCTGCATTATGAAAGTGGACTCTCTCACAGGAGCATTCCTCTTTTCCTTGGAATCTCAGAC AACCATTGGCTACGGGGTCCGTTCCATCACAGAGGAGTGTCCCCATGCTATCTTCCTCTTAGTCGCCCAA CTGGTCATCACCACATTGATTGAGATCTTCATTACGGGGACCTTTCTGGCTAAAATTGCAAGACCCAAAA AGCGAGCCGAGACCATTAAGTTCAGCCACTGTGCTGTCATCAGCAAGCAGAATGGAAAGCTATGCCTGGT CATCCAGGTGGCCAACATGAGGAAGAGTCTCCTGATTCAGTGCCAGCTCTCTGGAAAACTCCTGCAGACA CACGTCACCAAAGAGGGAGAACGCATTCTCCTCAACCAGGCCACTGTCAAATTCCACGTGGACTCCTCTT CCGAGAGTCCCTTCCTCATCCTGCCCATGACCTTCTACCACGTGTTGGATGAGACAAGCCCCCTGCGGGA CCTCACACCCCAAAACCTAAAGGAGAAGGAGTTTGAGCTGGTGGTACTTCTCAACGCCACGGTGGAGTCT ACCAGCGCCGTCTGCCAGAGCCGAACGTCTTACATCCCGGAGGAGATCTACTGGGGCTTTGAGTTTGTGC CTGTGGTTTCTCTCTCCAAAAATGGAAAGTATGTGGCTGATTTCAGTCAATTTGAGCAGATCAGGAAGAG CCCGGATTGTACCTTCTACTGTGCCGATTCTGAGAAGCAGAAGCTTGAAGAACAGTACAGGCAAGAGGAC CAGAGGGAGCGGGAGCTGAGGAGCCTCCTGCTACAGCAGAGCAATGTCTGACTCCACCGGCCTCTGGGCG TCTCCTAACCAGGGCTCCTTTCAAAAGCAATGGCTGCCATGAACGTGGGATGGGTGGCCCCACTACCAAA TCACACAGACGTTCGTGGCCATCTTCCCCTTTGTTCTGATGGCTGAACAAGCATTTCTGAATATAAAAAG ATGTCTTCAGAGTGCCTGGTCAGAGGTGAGCACTCAAATTCAGATTTCCCCCCACCCCTAAAATGGAGCT ATACATTGAAGAACTGAGTGTAGGAAGATGGAGAGAATTCCCGCTGGAGGGCAGACCCAGCAGTGCTGAC AGTGGATGGCATGTATGCCTACTCAAGAACATCTGCTCATAATGCAAAATATTTATTTACCCACCTAACA AGGGCATTGGTGGCTGAGGTCTGAGGCGTAATTTGCTATTTGCTCATAGCACTGCTTAAGAACTCACTCC TTCTCATGTTCAAGTGCATACGTTTTTATTTGCAATGACGGTTATCTGGAGATGACAAAAAAAAAAAAAA A
• Restriction Sites: RsrII-NotI
• ACCN: BC010794
• Insert Size: 1128 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC010794, AAH10794
• RefSeq Size: 1891 bp
• RefSeq ORF: 1128 bp
• Locus ID: 16516
• Cytogenetics: 16 55.86 cM
• Gene Summary: Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium.[UniProtKB/Swiss-Prot Function]