Gorasp1 (NM_028976) Mouse Untagged Clone

CAT#: MC201037

Gorasp1 (untagged) - Mouse golgi reassembly stacking protein 1 (Gorasp1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gorasp1
• Synonyms: 5430411C10Rik; GOLPH5; GRASP65; P65
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC012251 sequence for NM_028976
  CCCCACGTGACTAGGCCACGCAGCAGCGGGAGAGGGCGGCCATGGGGCTAGGGGCAAGCAGCGAGCAGCC GGCGGGCGGCGAGGGCTTCCATCTGCACGGGGTACAAGAGAACTCGCCGGCCCAGCAGGCAGGCCTGGAG CCCTACTTCGACTTCATCATCACCATCGGGCACTCGAGGCTGAACAAGGAGAACGACACGCTGAAGGCAT TGCTGAAGGCCAATGTGGAGAAGCCGGTGAAGCTGGAGGTATTCAACATGAAGACCATGAAGGTGCGCGA GGTAGAGGTGGTGCCCAGCAACATGTGGGGCGGCCAGGGCCTCCTGGGAGCCAGCGTGCGCTTCTGTAGC TTCCGCAGGGCCAGCGAACACGTGTGGCATGTGCTGGATGTGGAGCCCTCTTCACCTGCTGCCTTGGCCG GCCTGTGCCCTTACACAGACTACATAGTTGGCTCTGACCAGATTCTCCAGGAGTCAGAAGACTTCTTTAC TCTCATTGAGTCCCATGAGGGGAAGCCTCTGAAGCTGATGGTGTATAACTCGGAGTCCGACTCCTGCCGG GAGGTGACTGTGACTCCCAATGCAGCCTGGGGTGGAGAGGGCAGTCTGGGGTGTGGTATTGGTTATGGGT ACCTGCATCGGATCCCAACGCAACCCTCCAGCCAGCACAAGAAGCCACCCGGTGCCACACCACCTGGCAC TCCAGCTACGACCTCACAACTTACTGCCTTTCCTCTTGGTGCCCCACCACCTTGGCCTATCCCTCAGGAC TCTTCTGGCCCAGAGTTGGGTTCCAGGCAGAGTGACTTCATGGAGGCCCTACCACAAGTCCCTGGTAGCT TTATGGAGGGACAGCTCCTTGGGCCTGGGAGTCCCAGCCATGGCGCTGCTGACTGTGGGGGATGCCTGCG TGCTATGGAGATCCCGCTTCAGCCTCCACCTCCAGTGCAGCGGGTCATGGACCCAGGCTTCCTGGATGTA TCAGGCATGTCCCTCCTGGACAGCAGCAACATAAGCGTGTGCCCCAGCCTGTCATCTTCCACAGTGCTGA CCTCCACAGCTGTTTCAGTCTCAGGACCAGAGGACATTGGTTCTAGCAGCAGTTCTCACGAGCGGGGTGG TGAAGCCACGTGGTCAGGGTCAGAGTTTGAGATCTCCTTCCCAGACAGTCCAGGTGCCCAGGCCCAGGCG GACCACCTGCCCCGGCTGACTCTCCCCGATGGCCTCACATCTGCGGCCTCACCTGAAGAAGGGCTGTCTG CAGAGCTGCTGGAAGCACAGACTGAGGAGCCGGCAGACACAGCCAGCCTGGATTGCAGGGCAGAGACTGA GGGGAGAGCCAGCCAAGCGCAGGCCACCCCAGATCCAGAGCCTGGGCTGTGAGAACACCCCTGGTGACAT TTGGTGTGGCTTAGCTGGGTAGCTCTCTAGGCTGCAGGCTCTTGTGAGGCACGGGCTTTGCTACAATACA TACATGGTCCTGGGTCGAAGGGACAGCACACCCGAGCAGCAACCAGCTTCTGTAGAGGCAGGTGACGGAA TCTAGACTGTCAATGAAGCATCGATGCCCCATCACAATATCCAGGTGAAGATGGGGCGAGGGACAAGAGG CTGTGTCCATAGGCAGCCACACCTGTACTGAGGAAGACCCTAAGTCCCAATGCCCTTCCTCACTCTAATC CTTACACTGGATGAGCGAGGTGGTATATCTGGCCACTTTATTTCTTAGGATGGGAAATTCTAGGGATTTA CCTTCAGAGGGATCTACCTCCGCTAGCTGTTCACAGCCCCAGGGGGAGGTACAAAGGAGCTTACACTCCA GTTCTTGGGTCCTATGACGCTCCCTTCAGCCCTCACGTAGACAGCTGGCTCTGGTGGCAGACGGGTCAAA CTACGCTTCCTGTCAAAACCCCTCCACCTGCCCGTGCTCAGGGTTCTTCTCCTGAAGAGCTGGAAAAGGC CCCTCCCACCTGTCCTCAGCGTAAGGGAAAGGAGCCTTTACAGGCTCAGTCTCCGAGAGGACAGCTGCAC CGCTGCCTTGCTACAGATGGTGCCAGGCATGTCCTCTGCAGCCCCAGTTCCATGTCCTTTGCCAATCACT GTAAGCTGCCTTTGGTCTTCTTTTCCAGCCTCTTTGTTCCATCATCCACAGACCATGTAACTGTCTGTTC AGGGTACTTAGGCTGGTAAGCCAAGGACTCTGTTCCAAGAAGCACAGGCCAAATGGTTCAGTGGGCCAGG TCCTGGATGGGCAAACAGAACCCAAAGCACTAGGAATACATTTATAGCCAGGCCTGTGAGGCCCCCGCTC CCCAGGGGCCACTGAGAGGCCTGAGGCCGTACAACATGGCTTACCACAGGGCTGCTCTGGGGCCAGCTCA ATTTAGCTGTGGTTAGGCACTCTGACCCCCAGCTGCCTTCATTGGAAGGTTCTGCTCCCCAGGACCCCAT GTGGGGTGAAACGGAGGCTGATGCTGTTTCAAGTATGAACCCCCAGATGGTTTCTTTCCCTGTAATCTGC TATGGGGAAAGTGACAAGTGAAAAAATAAACATGTCCAGCACACTGAAGTGTTTGTAACTAAAGCTTTAT TCTATACAACCCGAGATACAGACTCTGCCCCTTTGGCGTTGCAGACTGTCCAGTTTTCTGGAAACTTAGT ACTGATTTTTGACTAGCTGGGGAAAAAATTACAACCATGCTGGGGTAAACTCCTCCATAGGGGCACAACT GCCTGTGGATCAGTACTGCGCCAGTGAACCAACCAGCAAACCCTACGCTTAGTGCAGAGGCCCCTCCTGC AAACACGACCACCCCACCCCCTGCTTTTACTTTATAGCTATCACTAAAAAATATGCACCAAAAGTGCACC GATGTGTCCAGCATAGAAAGGACTTCTGTAGAAAAGCTGGCTACTCTAGGGCGGAGAATGGTTTAACACT GGCAAGTGTTCTTGCTCACTTGAGGGTATGGAAGCCATGGCCTGTCTGTGTTCCAGAGTTAAGTGAGGGG ACCCTGTTCTGCAGTCAATTTGTAAGGGACAGATCTGGGCCCCTCAGCTGTTCCCTGCAGGACCTGTGAG ATAGAGAGAAAACATCTTTCTTCCCCAAGCCCCCCTCCCCTGTGCTCCTGTGGGCAGGGACCACACACAC AAGGCAGGAGCAGACGGACGGATGGGGAAAGCTGCACCCCCCACCCCCAAGGCACGTGTGCTTCCGCTGT GCTCAAAGGTTCATCTCCTGCAGCTTCACGGGCTGGACCATTTGTCTGAATGTAAATGACCTTGGGAAGA ATCCAAAGTAGTTTTTGCCAAAAGTTGAAGTGTTTATCCCCAATAAGTTAATATTCTGTACAAGATCCAA GTGAGTCTATGAAAGCTTTTGACTGTTAGAAAGACGGCAGAAAGACCTGGACGAAGGCGTAACAAAAGCC CGTGTATAAAACTGGAAAAACAAACATGAACCACAATATAAATATCAAACCAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_028976
• Insert Size: 1341 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC012251, AAH12251
• RefSeq Size: 3495 bp
• RefSeq ORF: 1341 bp
• Locus ID: 74498
• UniProt ID: Q91X51
• Cytogenetics: 9 F4
• Gene Summary: Plays an important role in assembly and membrane stacking of the Golgi cisternae, and in the reassembly of Golgi stacks after breakdown during mitosis. Key structural protein required for the maintenance of the Golgi apparatus integrity: its caspase-mediated cleavage is required for fragmentation of the Golgi during apoptosis (By similarity). Also mediates, via its interaction with GOLGA2/GM130, the docking of transport vesicles with the Golgi membranes (By similarity). Mediates ER stress-induced unconventional (ER/Golgi-independent) trafficking of core-glycosylated CFTR to cell membrane (By similarity).[UniProtKB/Swiss-Prot Function]