Hck (NM_010407) Mouse Untagged Clone

CAT#: MC200941

Hck (untagged) - Mouse hemopoietic cell kinase (Hck), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Hck
• Synonyms: AI849071; Bmk
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC010478 sequence for NM_010407
  CCCACGCGTCCGCCCACGCGTCCGCCCACGCGTCCGGCGCCACTGGGTTGCGGCTCCCAGCTTGCACTCC CCGCGGCGGCTCTCAGACGGGTGGGAGGGGACCAAAGTCGCCCGTGAAGGGGGCTCTGACCCGTTCCGAG CGCCAACGCAGCCTCTGTAGCCCGCAAGTCTTCGTCGCTTGCTCCGGGCTCTGAAGTCCGGGGCCACCAG GGGCCGCAGCGCTGGGGGGTCGGTCTAGCTGCGAGGATCCGGGCTGCCCACGAAGCGAAGGGCGGGCGCC CAGGATGGGATGCGTGAAGTCCAGGTTCCTCCGAGATGGAAGCAAGGCCTCAAAAACAGAGCCAAGTGCC AATCAGAAGGGCCCTGTGTATGTGCCGGATCCCACGTCCTCCAGCAAGCTGGGACCAAACAACAGCAACA GCATGCCCCCAGGGTTTGTGGAGGGCTCTGAGGATACCATTGTGGTCGCACTGTACGACTATGAGGCTAT TCACCGTGAAGACCTCAGCTTCCAGAAGGGAGACCAGATGGTGGTTCTGGAGGAGGCTGGGGAGTGGTGG AAGGCACGGTCCCTGGCTACCAAGAAGGAAGGCTACATCCCAAGCAACTATGTGGCTCGAGTTAACTCTT TGGAGACAGAAGAGTGGTTCTTCAAGGGGATCAGCCGGAAGGATGCAGAGCGCCACCTCCTGGCTCCAGG CAACATGCTGGGCTCCTTCATGATCCGGGACAGTGAGACCACCAAAGGGAGCTACTCGTTGTCTGTTCGA GACTTTGACCCCCAGCACGGAGACACCGTGAAGCACTATAAGATCCGGACGCTGGACAGTGGAGGCTTCT ACATCTCTCCAAGGAGCACCTTCAGCAGCCTGCAGGAACTCGTGCTCCACTACAAGAAGGGGAAGGATGG GCTCTGCCAGAAGCTGTCAGTGCCCTGTGTGTCTCCCAAACCCCAGAAGCCATGGGAGAAAGATGCTTGG GAGATTCCTCGAGAATCCCTCCAGATGGAGAAGAAACTTGGAGCTGGGCAGTTTGGAGAAGTGTGGATGG CCACCTACAACAAGCACACCAAAGTGGCGGTGAAGACAATGAAGCCAGGGAGCATGTCCGTGGAGGCCTT CCTGGCTGAGGCCAACCTGATGAAGTCGCTGCAGCATGACAAACTGGTGAAGCTACACGCTGTGGTCTCT CAGGAGCCCATCTTTATTGTCACGGAGTTCATGGCCAAAGGAAGCCTGCTGGACTTTCTCAAGAGTGAAG AAGGCAGCAAGCAGCCACTGCCAAAACTCATTGACTTCTCAGCCCAGATCTCAGAAGGCATGGCCTTCAT TGAGCAGAGGAACTACATCCACCGAGACCTGAGGGCTGCCAACATCTTAGTCTCTGCATCACTGGTGTGT AAGATTGCTGACTTTGGACTGGCACGAATCATCGAGGACAATGAGTACACAGCTCGGGAAGGAGCCAAGT TCCCCATCAAGTGGACAGCTCCTGAAGCCATCAACTTTGGTTCCTTCACCATCAAGTCAGATGTCTGGTC CTTTGGTATCCTGCTGATGGAAATTGTCACCTATGGCCGGATCCCTTACCCAGGTATGTCAAACCCAGAG GTGATTCGGGCACTAGAGCATGGGTACCGTATGCCTCGACCAGATAACTGTCCAGAAGAGCTCTACAATA TCATGATCCGCTGCTGGAAGAACCGCCCCGAGGAACGGCCCACCTTTGAATACATCCAGAGTGTGCTGGA TGACTTCTACACGGCCACTGAGAGCCAGTATCAGCAGCAGCCTTGACAGCAGTAAGGACATGAGCAGAGC CAGAAGCCCCATCAGTGCCTTGACACGCCCAACTTGCTGGGCCCACTCTCAGACACCACACCACACACAC TGCAGCTGTTGAGTGGGTGGGAGGACTTCACAATCTCTTTCTGACTCTAGTCATCTGCAATCCGCCACTC TCAGGGCCTCCAAGTTGGTATGTCTCATTTGCCTGGAATGACTGAATTCAATCTATAGCTGTGATTTAAG TGGAAACTGTTAGAATAGTATTTAAATAAAAGATATGAATGTCAAAGGCTTTCAAAAAAAAAAAAAAAAA AAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_010407
• Insert Size: 1575 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC010478, AAH10478
• RefSeq Size: 2104 bp
• RefSeq ORF: 1575 bp
• Locus ID: 15162
• UniProt ID: P08103
• Cytogenetics: 2 75.41 cM
• Gene Summary: The protein encoded by this gene is a member of the Src family of tyrosine kinases. This protein is primarily hemopoietic, particularly in cells of the myeloid and B-lymphoid lineages. It may play a role in the innate immune response and the STAT5 signaling pathway. Alternative translation initiation site usage, including a non-AUG (CUG) codon, results in the production of two different isoforms, that have different subcellular localization. [provided by RefSeq, Feb 2010]
  Transcript Variant: This transcript (1) encodes multiple isoforms due to the use of alternative translation initiation codons, as demonstrated in PMID:1875927. The longer isoform (p59Hck) is derived from an upstream non-AUG (CUG) start codon, while the shorter isoform (p56Hck) is derived from a downstream AUG start codon. The longer isoform (p59Hck) is represented in this RefSeq. CCDS Note: This CCDS ID represents the longer mouse Hck isoform, known as p59Hck, as described in PMID:1875927. This isoform initiates translation from a non-AUG (CUG) start codon that is well-conserved and present in a strong Kozak signal context. Alternative translation initiation from a downstream AUG start codon produces an isoform that is 21 aa shorter at the N-terminus. The shorter isoform, which is known as p56Hck, is represented by CCDS 50756.1. These isoforms exhibit distinct subcellular localization, as indicated in PMID:1875927.