Uhrf1 (NM_010931) Mouse Untagged Clone

CAT#: MC200929

Uhrf1 (untagged) - Mouse ubiquitin-like, containing PHD and RING finger domains, 1 (Uhrf1), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Uhrf1
• Synonyms: AL022808; ICBP90; Np95; RNF106
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC022167 sequence for NM_010931
  CCACGCGTCCGCTGGAAGGAACCCGCGCTCTAGTGCTCACTTGGGTCTTCAGCCACTCACGCGGCTCCCT TCTGGGTCACCCAGCCGCAGAGCCCTAGCCTAGAACCAGGCGTTCCAAGGGAGAGGAGAGTGCGGATCGC CGCCGAGAGAGAGTACATCGGCATCATGTGGATCCAGGTTCGAACTATGGATGGGAAGGAGACCCACACC GTGAACTCTCTGTCCAGGTTGACCAAGGTGCAGGAGCTGAGGAAAAAGATTGAGGAAGTGTTTCACGTGG AACCCCAACTGCAGAGACTCTTTTACAGGGGCAAACAGATGGAGGACGGCCACACACTCTTCGATTATGA TGTGCGCCTCAATGACACAATCCAGCTGCTCGTGCGCCAGAGTCTGGCACTGCCTCTCAGTACAAAAGAA CGGGACTCGGAGCTCTCAGACTCGGACTCTGGCTATGGTGTGGGTCACAGTGAATCAGACAAGTCGTCCA CGCATGGTGAAGGGGCAGCTGAAGCGGATGACAAGACTGTGTGGGAGGACACGGACCTGGGGCTGTACAA GGTCAATGAGTATGTGGACGTGCGTGACAATATCTTCGGTGCATGGTTTGAGGCCCAGGTGGTCCAGGTA CAGAAGAGAGCCCTATCTGAGGACGAGCCCTGTAGCTCCAGTGCCGTTAAGACCTCGGAGGATGACATCA TGTACCATGTCAAGTATGATGACTATCCAGAGCATGGAGTGGACATTGTCAAAGCCAAGAATGTCCGTGC TCGTGCTCGCACTGTGATACCATGGGAGAACCTGGAGGTGGGTCAGGTGGTCATGGCCAACTATAACGTG GACTACCCCAGGAAACGCGGCTTCTGGTATGATGTTGAGATCTGTAGGAAGCGCCAAACCAGGACGGCAC GTGAGCTATACGGCAACATCAGGCTCTTGAATGACTCTCAGCTCAACAACTGTCGGATCATGTTTGTGGA TGAAGTCTTGATGATTGAGCTCCCTAAAGAGAGGAGGCCTTTGATCGCTAGCCCCTCGCAACCCCCTCCT GCCCTCCGGAACACAGGGAAGAGCGGCCCATCCTGCCGGTTCTGCAAGGATGATGAAAACAAACCATGTC GAAAGTGTGCCTGCCACGTGTGTGGTGGGCGCGAGGCTCCTGAGAAACAGCTGTTGTGTGATGAGTGTGA CATGGCCTTCCACCTGTACTGCCTGAAGCCACCGCTCACCTCTGTCCCCCCTGAGCCGGAGTGGTACTGC CCCAGCTGCCGCACTGACTCAAGTGAGGTGGTACAAGCAGGGGAGAAGCTGAAGGAGAGCAAGAAGAAGG CGAAGATGGCATCAGCCACTTCCTCCTCCCGGCGGGACTGGGGCAAGGGCATGGCATGTGTGGGCCGCAC CACAGAGTGTACCATTGTGCCCGCCAACCACTTCGGGCCCATCCCTGGTGTCCCTGTGGGCACCATGTGG CGCTTCAGAGTCCAGGTCAGTGAGTCCGGTGTGCATCGGCCTCATGTGGCAGGCATCCATGGCCGGAGCA ACGACGGTGCCTACTCATTGGTCCTGGCTGGTGGCTATGAGGATGATGTGGACAATGGCAATTACTTCAC ATACACAGGGAGTGGTGGCCGAGACCTCTCTGGCAACAAGCGTACAGCAGGCCAGTCCTCTGACCAGAAG CTCACTAATAACAATAGGGCTCTGGCACTCAATTGCCACTCCCCAATCAATGAGAAAGGTGCGGAGGCTG AAGACTGGCGCCAAGGGAAGCCAGTGCGTGTGGTCCGGAACATGAAGGGCGGGAAACACAGCAAGTACGC TCCTGCAGAGGGCAACCGCTATGATGGCATCTACAAGGTGGTGAAGTACTGGCCAGAGAGAGGGAAATCT GGCTTCCTCGTGTGGCGTTATCTCCTTCGACGAGATGACACAGAGCCTGAGCCCTGGACCCGGGAGGGCA AGGACCGCACTCGACAGCTGGGGCTCACTATGCAGTACCCTGAAGGCTACTTGGAGGCCTTGGCTAACAA GGAGAAGAGCAGGAAGCGCCCGGCCAAGGCCTTGGAGCAGGGACCCTCATCTTCCAAGACAGGCAAAAGC AAGCAGAAGTCCACAGGGCCGACCCTCTCCAGCCCCCGTGCCTCTAAGAAGAGCAAGCTGGAGCCGTACA CACTCTCAGAGCAGCAGGCTAACCTCATCAAAGAGGACAAGGGCAACGCCAAGCTGTGGGATGATGTGCT AACTTCACTTCAGGATGGGCCGTACCAGATCTTCCTGAGCAAGGTGAAAGAGGCTTTCCAGTGCATCTGC TGCCAGGAGCTGGTGTTCCGGCCAGTCACCACCGTGTGTCAGCACAACGTCTGTAAGGACTGTCTGGACA GGTCCTTCCGGGCCCAGGTGTTCAGCTGCCCCGCCTGTCGCTATGAGCTGGACCACAGCTCCCCAACCCG GGTGAACCAGCCCTTGCAGACCATTCTCAACCAGCTCTTCCCTGGCTATGGCAGCGGCCGGTGATGCTGA GACCCAGGCAGAGGGCTCATGGTTCCAACTTCATAGTGTGTTTAGCTTGAAGGTGTTGTCCTTCACGTCT GCCTGTGGCAACTCTTCTGATCTGGTCACCAGTTAACCAGGCATCTATAGGAATCCCATGTTCTGGCTAC CAGTTGGACTTCTGTTTTTCATCCCTCCACCCCCTTTCTATAACAAAGCCTGCGATTTATTGTCCAAGAG AAGGAAAAAAATGTTTTTGTTTTTGTTTTTGTTTTTTTTTGAGATGGGTTTAGAAACCGTTGAGTTTCAT ACCAGGCCTCATGTCATGCTGGTAGGAGTTCTCCCCACACCAGCTGCCTAGAAACCATCTGCACCCATCT TTGAAAAGCCCAGACATGTCTTCCTGGCAGCTATCTGTGTGCTTCATTCCAGGGGTGGCCCCTGCAGCCG CCCCTCAGACCTTCATAGATGCCCTGGCCCGCGCCATTCACTGACATGATTGCAGAGGGAGTTGGCTGTG GCCTCAGGCCAGGGGAGCAGCTGGGCTGAAGCTGCATGTGGCTTGGGCTGGCCACTTCTTCACTCCTCAC CACTGAGCACTGGATGCCCACCGTGGAAGCCTGGTTTTCAGAGAGTGCACTGGCTGGGTGTCATGGAAGG CTGAGATGTGTTATCAGTTCCATCTCGTTTTTAACTCAGCAACAGAATTCTGAGGGTTTCTGTATTAATG GGTTTTTTTATTTTTATTTTTTAAAGCGAGATAAATGGGTTTCCTCGATGAATTTCTTTTGTAGTTACAG CCTGTACTGAGGAAGATGTTTTCCTTGGTAGGTTTTGTTTCTTTTGTAACTTTACCAAAGTGTGCAGGTG TGTCTCATGGAAACTGAACCATTTACTACAGGCAGACTGGACAGCACAGTTCTAAGGGTTGTGTTTTGTT TTGTGTGGGGTATTTTTTCCTTGTTAGATTGTTAACATATTGCAATAAAATAAAATTCTGGCCGGGCAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_010931
• Insert Size: 2349 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC022167, AAH22167
• RefSeq Size: 3555 bp
• RefSeq ORF: 2349 bp
• Locus ID: 18140
• UniProt ID: Q8VDF2
• Cytogenetics: 17 D
• Gene Summary: Multidomain protein that acts as a key epigenetic regulator by bridging DNA methylation and chromatin modification. Specifically recognizes and binds hemimethylated DNA at replication forks via its YDG domain and recruits DNMT1 methyltransferase to ensure faithful propagation of the DNA methylation patterns through DNA replication. In addition to its role in maintenance of DNA methylation, also plays a key role in chromatin modification: through its tudor-like regions and PHD-type zinc fingers, specifically recognizes and binds histone H3 trimethylated at 'Lys-9' (H3K9me3) and unmethylated at 'Arg-2' (H3R2me0), respectively, and recruits chromatin proteins. Enriched in pericentric heterochromatin where it recruits different chromatin modifiers required for this chromatin replication. Also localizes to euchromatic regions where it negatively regulates transcription possibly by impacting DNA methylation and histone modifications. Has E3 ubiquitin-protein ligase activity by mediating the ubiquitination of target proteins such as histone H3 and PML. It is still unclear how E3 ubiquitin-protein ligase activity is related to its role in chromatin in vivo. May be involved in DNA repair.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (A). Variants 1 and 2 encode isoform (A).