Prkcsh (BC009816) Mouse Untagged Clone

CAT#: MC200908

Prkcsh (untagged) - Mouse protein kinase C substrate 80K-H (cDNA clone MGC:13908 IMAGE:4008182), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Prkcsh
• Synonyms: 80K-H; PKCSH
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC009816
  CCACGCGTCCGATTGACCTTTGCCTCTACCTCGTTGAGAGGCCATCGCGTATCTTCCTGCCGCATAACAC ACTTCCGGTCTCGGCCCGGACGGGATGCTGCTGCTGCTGCTACTACTACTACCCCTCTGTTGGGCTGTAG AAGTCAAGAGACCCCGGGGCGTTTCCCTCAGCAACCATCACTTCTATGAAGAATCTAAACCTTTCACCTG TTTGGACGGCACAGCCACCATCCCATTCGATCAGGTGAACGACGACTACTGCGACTGTAAGGACGGTTCA GATGAGCCTGGCACAGCTGCTTGTCCCAATGGCAGCTTTCACTGCACCAACACTGGGTACAAGCCCTTGT ACATCCTCTCCAGCCGGGTCAATGATGGGGTATGTGACTGCTGTGATGGCACAGACGAGTACAACAGCGG CACGGTCTGCGAGAACACCTGCAGAGAGAAGGGTCGCAAGGAAAAAGAGTCCCTGCAGCAGCTGGCGGAA GTCACCCGTGAAGGGTTCCGCCTGAAGAAGATTCTCATTGAGGAGTGGAAGACAGCCCGGGAAGAAAAGC AGAGTAAGCTTCTTGAGCTTCAGGCTGGAAAGAAGTCTCTGGAAGACCAGGTAGAAACACTGCGGGCAGC GAAAGAAGAAGCAGAGAGGCCAGAGAAGGAGGCCAAGGACCAGCACCGGAAGCTGTGGGAAGAGCAGCAA GCTGCTGCCAAGGCCCGGCGGGAACAGGAGCGGGCAGCCAGTGCCTTCCAGGAACTTGACGACAACATGG ATGGGATGGTCTCGCTGGCTGAGTTACAGACTCACCCGGAGCTGGACACAGATGGAGATGGAGCGCTGTC TGAGGAGGAGGCCCAGGCCCTTCTCAGTGGAGACACACAGACTGACACCACCTCCTTCTATGACCGTGTC TGGGCTGCCATCAGGGACAAGTACCGCTCTGAGGTCCCGCCCACTGACATACCTGTTCCGGAGGAGACTG AGCCCAAAGAGGAAAAGCCACCAGTGTTGCCACCCACAGAGGAGGAGGAAGAGGAGGAGGAGGAGCCAGA AGAAGAGGAGGAGGAAGAGGAAGAGGAGGAGGTGCAGGGGGAGCAGCCCAAGGAGGCTCCGCCCCCACTG CAGCCCCCACAGCCTCCCAGCCCCACAGAGGATGAGAAGATGCCGCCCTATGATGAGGAGACCCAGGCCA TCATCGATGCTGCACAGGAGGCCCGGAGTAAGTTTGAGGAAGTCGAACGGTCCTTGAAAGAGATGGAAGA GTCCATCAGGAGTTTGGAACAAGAGATCTCCTTTGATTTCGGTCCCTCTGGAGAGTTTGCATATCTCTAC AGCCAATGCTACGAGCTCACCACCAATGAGTACGTCTACCGGCTTTGCCCCTTCAAACTGGTCTCCCAGA AACCCAAACATGGGGGCTCCCCGACCAGCCTGGGCACATGGGGCTCCTGGGCTGGCCCTGATCATGACAA GTTCAGTGCGATGAAGTACGAGCAGGGCACGGGCTGTTGGCAGGGCCCCAACCGATCCACCACAGTGCGC CTGCTGTGTGGCAAAGAGACTGTGGTGACCAGCACCACGGAGCCCAGTCGCTGTGAGTACCTCATGGAGC TGATGACACCAGCAGCCTGCCCAGAGCCGCCACCAGAAGCACCCAGTGATGGGGACCATGACGAGCTGTA GCCTGAACCTCAAGGCCTGCAACCAGTCTACTGATCCTGAAGTCTGCAGACCAAGGCCTGCCCACCCTGG GCTTCCTGGCCATCCTCTGGGAAGCAGGAACCCTGAGGGGCTCTAAGCCCCACTTCTGGCCCAGGCTTGC CAAGCCATGTTCCTGAGGCCCCAGTGGCCTCCAGAGATGAATAATGAGCTCGCCTACCTTGGATTCCTAA GGGGAGTTAGTCCCTGCACCCACCTCACCCCTGCCTCCCCGGCTGGTGGGAGATAAATGGATGACTTGAC CTGTGACCTCAGAACAATAAATGTGACTTGTCCAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: BC009816
• Insert Size: 1587 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC009816, AAH09816
• RefSeq Size: 2011 bp
• RefSeq ORF: 1587 bp
• Locus ID: 19089
• Cytogenetics: 9 8.04 cM
• Gene Summary: Regulatory subunit of glucosidase II that cleaves sequentially the 2 innermost alpha-1,3-linked glucose residues from the Glc(2)Man(9)GlcNAc(2) oligosaccharide precursor of immature glycoproteins (PubMed:27462106, PubMed:9148925). Required for efficient PKD1/Polycystin-1 biogenesis and trafficking to the plasma membrane of the primary cilia (PubMed:21685914).[UniProtKB/Swiss-Prot Function]