Galnt11 (BC011428) Mouse Untagged Clone

CAT#: MC200863

Galnt11 (untagged) - Mouse UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 11 (cDNA clone MGC:19374, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Galnt11
• Synonyms: A430075I06Rik; AI648252; E430002F06Rik
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC011428
  GTCGTGGGCCGCGTCGCGCGCCGCGGCTCGTGCGGCTCGGCCCGGGCTGTGGCGGGAGCGGAGATGCCGC AGCCCGTGCCCTGGAGCGCCGCTATCCCGGCCTGCGGGTGTCGCGGAAACTGAAAATGGCCTTTGAGCTA TGCTAGGTCTATAATGGGAAGCATCACAGTTCGATATTTTTGTTATGGGTGCCTTTTTACATCTGCTACC TGGACAGTTCTGCTTTTCATTTATTTTAACTTCAGTGAAGTGACTCAGCCCCTTAGGAATGTGCCCATCA AGGGGTCTGGGCCCCACGGGCCATTTCCCAAAAAATTCTACCCACGTTTCACTCGAGGTCCAGGTCGGGT ATTAGATCCACAGTTCAAAGCAAACAGAATCGACCGTTTGATGAATAATCACATTGAAGATCCAGATAAA GGCCTCTCAAAATCTTCCTCTGAACTAGGTATGATTTTCAATGAACGTGATCAGGAATTGAGAGACCTGG GCTACCAGAAGCACGCCTTTAATATGCTCATTAGTAACCGCTTGGGATACCACAGAGACGTGCCGGACAC CAGGAATGCAGAGTGTAGGAGGAAGTCCTACCCGACTGACCTGCCAACTGCTAGCATTGTCATCTGTTTC TACAATGAAGCCTTTTCTGCCTTACTGCGGACTGTGCACAGTGTTGTGGACCGCACACCGGCCCACCTTC TTCATGAGATCATCCTTGTGGATGACAGCAGTGACTTTGATGATTTAAAAGGAGAACTGGATGAGTACAT CCAAAGATACCTGCCTGCAAAAGTCAAGGTCATAAGAAATATGAAGCGCGAGGGATTGATCCGAGGACGA ATGATCGGAGCAGCCCACGCTACAGGAGAAGTCCTGGTGTTCCTGGACAGCCACTGTGAAGTGAATGTGA TGTGGTTACAGCCCCTGCTGGCCATTATCCTTGAGGACCCACACACAGTGGTGTGCCCCGTGATTGACAT CATCAGTGCAGACACACTCGCTTACAGCTCATCCCCAGTAGTTCGGGGAGGTTTCAACTGGGGGCTGCAC TTCAAGTGGGATCTAGTTCCCGTATCTGAGCTGGGAGGCCCTGACGGCGCCACGGCCCCAATAAGGTCAC CTACAATGGCTGGAGGCTTGTTTGCCATGAACAGACAGTATTTCAATGATCTTGGACAGTATGACAGTGG CATGGATATCTGGGGAGGAGAAAATCTGGAAATCTCATTTCGGATCTGGATGTGTGGCGGCAAGCTCTTC ATCCTCCCTTGCTCAAGAGTAGGACACATTTTCCGAAAAAGGCGACCGTACGGATCTCCGGAAGGCCAGG ACACCATGACACACAACTCCCTGAGACTGGCACATGTCTGGCTGGATGAGTACAAGGAGCAATATTTTTC CTTAAGACCTGATTTGAAGAACAAAAGCTTCGGGAATATCAGTGAGCGTGTTGAATTGAGGAAGAAACTG GGCTGTCAGTCATTCAAGTGGTATTTGGATAATATTTACCCTGAGATGCAGATACCTGGCCCGAATGCCA AACCTCAGCAGCCCGTTCTCATCAACAGAGGACCCAAGCGCCCCAGAGTCCTGCAGCGTGGAAGGCTCTA CCACTTGCAGACCAACAAATGCCTGGTGGCCCAGGGCCGCTCCAGCCAGAAAGGAGGCCTAGTGCTGCTT AAGACCTGTGACTACGGAGACCCAACTCAGGTCTGGATTTATAATGAAGACCATGAATTGATTTTAAACA ACCTCCTTTGCCTTGACATGTCAGAAACTCGCTCATCGGATCCACCCCGACTCATGAAGTGCCATGGGTC AGGAGGATCCCAGCAGTGGACCTTTGGGAAGAACAACCGGCTGTACCAAGTGTCCGTTGGGCAGTGCCTG AGAGTTATGGACCTGATGGATCAGAAGGGCTACGTTGGCATGGCGATCTGCGATGGCTCCTCCTCTCAAC AGTGGCGTCTAGAAGGTTAAAGGTGGGCCCTGGCTGGACACAACATAATTCACCTGGTTTTGTCATGCCT GCCATAAATCAAGGTGCTTTAAGGTTGCTGACTGCAGCAGACAAGAACCTTGGTGGAGGGCGACCATCTC AGGAGCGCCACTGGGATTTGGGTTTACTTCCGAGCTGCTGTTAAGGGATTCTTGTTTATCCAGGGAAAAC AGAAAATAATTCTAAAGAGTTGGGTCTACAGAGTATAAACCCAGGAAGTAGGCATTTGTATTCATATTTA TGTTTTTCGCATTCTCCCTTGGAGATGGAATTGTTTCTGTCCAATCAGGAACTTGTCACAATTTCCTTTC ATAGAGGACTTCATAAGAGATTTTTTTCTATTTCTATCATGCCTAATGTTCCAAATAGATTTTAAAAACT GGTTGAGTGCATATTACTTTTAGCTTCAGAAGATATCATGTATATTTAAGAGGCATTTAACTATTATAAA TTATTTTGATGACTTAATATTGTCTGTCTACATTAAATAAAGGATCTTTTTGATCATTTAAAAAAAAAAA AAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: BC011428
• Insert Size: 1827 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC011428, AAH11428
• RefSeq Size: 2539 bp
• RefSeq ORF: 1827 bp
• Locus ID: 231050
• Cytogenetics: 5 A3
• Gene Summary: Polypeptide N-acetylgalactosaminyltransferase that catalyzes the initiation of protein O-linked glycosylation and is involved in left/right asymmetry by mediating O-glycosylation of NOTCH1. O-glycosylation of NOTCH1 promotes activation of NOTCH1, modulating the balance between motile and immotile (sensory) cilia at the left-right organiser (LRO). Polypeptide N-acetylgalactosaminyltransferases catalyze the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Displays the same enzyme activity toward MUC1, MUC4, and EA2 than GALNT1. Not involved in glycosylation of erythropoietin (EPO) (By similarity).[UniProtKB/Swiss-Prot Function]