Pbef1 (BC018358) Mouse Untagged Clone

CAT#: MC200839

Pbef1 (untagged) - Mouse pre-B-cell colony-enhancing factor 1 (cDNA clone MGC:19365 IMAGE:2609271), (10ug)


Reconstitution Protocol

USD 457.00

In Stock*

Size
    • 10 ug

Product Images

Frequently bought together (3)
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Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Symbol Pbef1
Synonyms Nampt, Pbef, Visfatin
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>BC018358
CGGAGCCGCCGGGAAAGCCGCCGGAGCGCGCTGGGCAGGGGAGCGCGCTAGCCTCCACCGCCGCGTCACG GACCTGCCCGCCCTCCCGGCCCCTCCCCGCGCTCCGTTCCCTGCTCTCCCGCCCCCCTGCGAGCGAGCGG TGACTTAAGCAACGGAGCGCAGCGAAGCCCATTTTTCTCCTTGCTCGCAGCCGCGCCGGGCAGCTCGTGG CGCGGCGTCTCCGCTCCGGCCCGAGATGAATGCTGCGGCAGAAGCCGAGTTCAACATCCTGCTGGCCACC GACTCGTACAAGGTTACTCACTATAAACAATACCCACCCAACACAAGCAAAGTTTATTCCTACTTTGAAT GCCGTGAAAAGAAGACAGAAAACTCCAAAGTAAGGAAGGTGAAATACGAGGAAACAGTATTTTATGGGTT GCAGTACATTCTTAATAAGTACTTAAAAGGTAAAGTAGTGACCAAAGAGAAAATCCAGGAGGCCAAAGAA GTGTACAGAGAACATTTCCAAGATGATGTCTTTAACGAAAGAGGATGGAACTACATCCTTGAGAAATACG ATGGTCATCTCCCGATTGAAGTAAAGGCTGTTCCCGAGGGCTCTGTCATCCCCAGAGGGAACGTGCTGTT CACAGTGGAAAACACAGACCCAGAGTGCTACTGGCTTACCAATTGGATTGAGACTATTCTTGTTCAGTCC TGGTATCCAATTACAGTGGCCACAAATTCCAGAGAACAGAAGAAAATACTGGCCAAATATTTGTTAGAAA CCTCTGGTAACTTAGATGGTCTGGAATACAAGTTACATGACTTTGGTTACAGAGGAGTCTCTTCGCAAGA GACTGCTGGCATAGGGGCATCTGCTCATTTGGTTAACTTTAAAGGAACAGATACTGTGGCGGGAATTGCT CTAATTAAAAAATACTATGGGACAAAAGATCCTGTTCCAGGCTATTCTGTTCCAGCAGCAGAGCACAGTA CCATAACGGCTTGGGGGAAAGACCATGAGAAAGATGCTTTTGAACACATAGTAACACAGTTCTCATCAGT GCCTGTGTCTGTGGTCAGCGATAGCTATGACATTTATAATGCGTGTGAGAAAATATGGGGTGAAGACCTG AGACATCTGATAGTATCGAGAAGTACAGAGGCACCACTAATCATCAGACCTGACTCTGGAAATCCTCTTG ACACTGTATTGAAGGTCTTAGATATTTTAGGCAAGAAGTTTCCTGTTACTGAGAACTCAAAAGGCTACAA GTTGCTGCCACCTTATCTTAGAGTCATTCAAGGAGATGGCGTGGATATCAATACTTTACAAGAGATTGTA GAGGGAATGAAACAAAAGAAGTGGAGTATCGAGAATGTCTCCTTCGGTTCTGGTGGCGCTTTGCTACAGA AGTTAACCCGAGACCTCTTGAATTGCTCCTTCAAGTGCAGCTATGTTGTAACCAATGGCCTTGGGGTTAA TGTGTTTAAGGACCCAGTTGCTGATCCCAACAAAAGGTCAAAAAAGGGCCGGTTATCTTTACATAGGACA CCAGCGGGGAACTTTGTTACACTTGAAGAAGGAAAAGGAGACCTTGAGGAATATGGCCATGATCTTCTCC ATACGGTTTTCAAGAATGGGAAGGTGACAAAAAGCTACTCATTTGATGAAGTCAGAAAAAATGCACAGCT GAACATCGAGCAGGACGTGGCACCTCATTAGGCTTCATGTGGCCGGGTTGTTATGTGTGCAGTGTGTGTA TACATACATGCACGTATGTGTGCGCCTGTGCGTATGTACTAACATGTTCATTGTACAGATGTGTGGGTTC GTGTTTATGATACACTGCAGCCAGATTATTTGTTGGTTTATGGACATACTGCCCTTTTTATTTTTCTCCC AGTGTTTAGATGATCTCAGATTAGAAAACACTTACAACCATGTACAAGATTAATGCTGAAGCAAGCTTTT CAGGGTCCTTTGCTAATAGATAGTAATCCAATCTGGTGTTGATCTTTTCACAAATAACAAACCAAGAAAC TTTTATATATAACTACAGATCACATAAAACAGATTTGCATAAAATTACCATGCCTGCTTTATGTTTATAT TTAACTTGTATTTTTGTACAAACGAGATTGTGTAAGATATATTTAAAGTTTCAGTGATTTACCAGTCTGT TTCCAACTTTTCATGATTTTTATGAGCACAGACTTTCAAGAAAATACTTGAAATAAATTACATTGCCTTT TGTCCATTAATCAGCAAATAAAACATGGCCTTAACCAAAAAAAAAAAAAAAA
Restriction Sites RsrII-NotI     
ACCN BC018358
Insert Size 1476 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq BC018358, AAH18358
RefSeq Size 2292 bp
RefSeq ORF 1476 bp
Locus ID 59027
Cytogenetics 12 A3
Gene Summary The secreted form behaves both as a cytokine with immunomodulating properties and an adipokine with anti-diabetic properties, it has no enzymatic activity, partly because of lack of activation by ATP, which has a low level in extracellular space and plasma (By similarity). Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway. Plays a role in the modulation of circadian clock function. NAMPT-dependent oscillatory production of NAD regulates oscillation of clock target gene expression by releasing the core clock component: CLOCK-ARNTL/BMAL1 heterodimer from NAD-dependent SIRT1-mediated suppression.[UniProtKB/Swiss-Prot Function]

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