Cast (BC010603) Mouse Untagged Clone

CAT#: MC200735

Cast (untagged) - Mouse calpastatin (cDNA clone MGC:12116 IMAGE:3710078), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cast
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC010603
  GCAGTCGTCCCCTAAACCAAGCTGAGGCCAGTCGTCATACTCTGAGTGACAGCTTCCTCTGCTGCCACCA GCAAGTCCCCCAGCATGAGTACCACAGAGACTAAGGCTGTGAAAACAGAGTCTAAGAAACCACAATCAAG TGAGCAACCTGTGGTTCACGAGAAAAAGTCAAAAGGAGGGCCAAAGGAAGGGTCAGAGCCCAAAAATCTT CCGAAGCACACATCAAGTACAGGAAGCAAGCATGCTCATAAAGAAAAAGCACTTTCCAGATCAAATGAAC AGATGGTGTCAGAGAAACCATCAGAATCAAAGACAAAATTTCAAGATGTTCCTTCAGCTGGTGGAGAGAG TGTGGCTGGAGGTGGGACTGTGGCCACTGCCTTGGACAAAGTGGTTGGTAAGAAAAAAGAACAGAAACCA TTCACACCAGCTTCACCTGTGCAGTCCACACCGAGCAAGCCATCTGACAAGTCAGGCATGGATGCTGCCT TGGATGACCTGATAGACACCTTAGGTGGACATGAAGATACTAACAGAGATGATCCACCATATACTGGACC AGTAGTTTTGGACCCAATGTATTCTACCTACCTTGAGGCACTGGGTATAAAAGAAGGGACTATTCCTCCA GAGTATAGGAAACTTTTGGAAAAAAATGAAGGGATCACACAACCTCTTCCAGACTCTCCTAAACCTATGG GGACTGACCAGGCTATAGATGCCTTGTCATCTGATTTCACCTGTAGTTCTCCAACTGGAAAGCAAAGTGA GAAAGAGAAGTCTACTGGGGAAATTTTCAAAGCTCAGTCTGCAGGAGTGACCAGAAGTTCTGTTCCCCCC AAGGAGAAGAAAAGGAAGGTGGAAGAGGAGGTCATAAATGATCAAGCACTTCAGGCTCTGTCAGATTCAC TGGGCACCCGGCAGCCAGATCCTCCGAGCCACGTTAGCCAAGCTGAACAAGTCAAAGAGGCAAAAGCAAA AGAAGAAAGGCAGGAGAAGTGTGGTGAAGATGAGGACACAGTCCCAGCTGAGTACAGGTTAAAACCAGCA AAGGATAAAGATGGAAAACCACTATTGCCAGAGCCTGAAGAAACATCTAAGAGCCTGAGTGAGTCGGAGC TGATTGGGGAGCTTTCAGCAGATTTTGACCGATCTACATATCAAGACAAACCATCTACGCCAGCTGAAAA GAAATCCAATGACACATCCCAAACTCCTCCGGGGGAGACTGTGCCTCGGGCCTCCATGTGCAGTATACGG TCAGCGCCACCCAAACTAGCATCCTTGAAGGGCGTGGTACCAGAAGATGCTGTTGAAACCTTGGCTGGAA GCCTGGGGACAAGGGAAGCAGATCCAGAACATGAAAAAACTGTGGAGGATAAAGTCAAGGAGAAAGCTAA AGAAGAAGAGCATGAAAAACTTGGTGAAAAAGAAGAAACAGTGCCTCCTGATTATCGACTAGAAGAAGTC AAGGATAAGGATGGAAAACCACTCCTGCCCAAAGAATCCCAGGAACAACTTGCACCCTTAAGCGATGACT TCCTTCTTGATGCCTTGTCTCAGGACTTCTCCAGTCCGGCAAACATATCGTCTCTTGAATTTGAAGATGC CAAACTTTCTGCTGCCATTTCTGAAGTAGTTTCTCAGACACCTGCTCCAAGCACCCATGCAGCAGCTCCA CTGCCTGGCACTGAGCAGAAAGACAAAGAACTTGATGATGCCTTGGATGAACTTTCTGACAGTCTTGGAC AAAGGCCGCCTGATCCAGATGAGAACAAACCACTGGATGACAAAGTGAAGGAGAAAATCAAACCAGAGCA TAGCGAGAAACTGGGAGAAAGAGACGACACCATCCCCCCTGAATACAGGCATCTCTTGGATAATGATGGG AAGAAACCTGATCAGGACCGGGACCCCATTGATGCCCTCTCAGAAGATTTGGATAGCTGCCCCTCAACTA CAGAGACCTCAAAGAATACAGCAAAGGGGAAGAGCAAGAAGACTTCAAGTTCCAAAGCATCCAAGGACGG AGAGAAAACAAAGGACTCTTCCAAGAAGACAGAGGAAGTGTCCAAGCCAAAGGCTAAAGAAGATGCAAGA CACAGTTAATGAATTTGGGTATCTGAATAGAAATTCTTCAGCTGGTGGATGGTGACTTTTGAAGAACAAA GGCTTTGATGACAGTTTTTTCTGGGTGGTTTCTGGACAGTGGTATTTGTTATGTCTTTTGACATCTTAAA CACTGAGTTGTTATTCTTTTCCAGAACGAAAATGAATTTGTCTGCTTCACCTGTGTTCTGTATTGAGTAT TGATAAACTTCAAAGATTTTTAATTGCCCTTAGATGGGAGAAGGAAGCTTTATATTTGTAAGAAATATAT TTGATAGTTTCTTAAAACACCAAAAATCCCAGAAAGCCTTTACAAACCTTTGCACTTTATATATACAGTG CCTGTAAATCTCATTAGTATTTTTGGTTTGCACTAAATTTTTTTAGCATTTGAAAAATAATGCTCCCAAC TTTTGTCAGTATTTTGATTTCTTATTATGTCTTTTTCTTCAGCTTTGATGTATCTTTGGGTTAATGTTGA TAAGTCAAACATGAAATATTGTATACTCGGTGTATAACTCTTTTGGGGATGCTAATAATAAATTAATGAT AGATGACCCAAGGTAAACCTTGGGAGCATCAATCTCAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: BC010603
• Insert Size: 2025 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC010603, AAH10603
• RefSeq Size: 2712 bp
• RefSeq ORF: 2025 bp
• Locus ID: 12380
• Cytogenetics: 13 C1
• Gene Summary: This gene encodes an inhibitor of the calcium-dependent cysteine protease, calpain. This protein plays roles in multiple processes, including apoptosis, cell cycle regulation, and membrane fusion. Multiple protein isoforms exist which contain unique N-terminal domains, and multiple inhibitory domains that share homology with each other. Some isoforms may be tissue-specific. Two different pseudogenes of this gene are found on chromosome 19. [provided by RefSeq, Jul 2014]