Slc29a1 (NM_022880) Mouse Untagged Clone

CAT#: MC200560

Slc29a1 (untagged) - Mouse solute carrier family 29 (nucleoside transporters), member 1 (Slc29a1), nuclear gene encoding mitochondrial protein, transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Slc29a1
• Synonyms: 1200014D21Rik; AA407560; ENT1
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC006812 sequence for NM_022880
  GAGAAAGGCCCAGGGCAGTGTGGAAGCAGAGCCAGACAGGGCTCGATATTCCTTTACCCCCAGCAAGAGC CAGAGGGAGGGAGCTGCCAGCCAGGCACAGCCGAGAACACTGGAGCCATGACAACCAGTCACCAGCCTCA GGACAGGTATAAGGCAGTATGGCTTATCTTCTTTGTGCTGGGCCTGGGGACACTGCTCCCCTGGAATTTT TTTATGACCGCAACCAAGTATTTCACAAACCGCCTGGACGTGTCCCAGAATGTGTCCTCGGACACTGATC AATCATGCGAAAGCACCAAGGCCTTGGCTGACCCCACAGTGGCCTTGCCAGCCCGGAGTTCTCTCAGTGC CATCTTCAACAATGTCATGACCCTGTGTGCCATGCTGCCCTTGCTGGTCTTCACCTGCCTCAACTCGTTT CTGCATCAGCGGATCTCTCAATCTGTTCGGATCTTGGGCAGCCTGCTGGCAATCCTGCTGGTATTCCTTG TCACTGCCGCCCTGGTGAAGGTGGAGATGGATGCTCTGATCTTCTTTGTCATCACCATGATCAAGATTGT GCTCATCAATTCATTTGGTGCCATTTTGCAAGCCAGCCTTTTTGGTCTGGCAGGTGTCCTGCCAGCCAAC TACACAGCCCCCATCATGAGTGGCCAGGGCCTGGCTGGCTTCTTCACCTCTGTCGCCATGATCTGTGCCA TTGCCAGTGGTTCTGAGCTGTCAGAAAGCGCCTTTGGCTACTTCATCACAGCCTGTGCAGTTGTCATTTT GGCCATCCTGTGCTACCTGGCTCTGCCTCGGACGGAATTCTATCGCCATTACCTGCAGCTCAACCTTGCG GGGCCTGCAGAGCAGGAGACCAAGTTGGATCTCATAAGAGAGGAGCCAAAAGGAAGAAGAGAGGAATCTG GGGTGCCAGGCCCCAACTCTCCACCCACCAACAGAAACCAGTCTATCAAAGCCATACTTAAGAGTATCTG TGTCCCGGCTCTGTCTGTCTGCTTCATCTTCACGGTTACCATTGGGTTGTTCCCTGCTGTGACTGCTGAG GTGGAATCCAGCATCGCAGGCACAAGTCCCTGGAAAAGCTACTTCATTCCCGTGGCCTGTTTCTTGAATT TCAATGTCTTTGACTGGCTAGGCCGGAGCCTCACTGCTGTCTGCATGTGGCCTGGCCAGGATAGCCGCTG GCTGCCGGTTTTGGTCGCCTCGAGGATTGTGTTTATTCCCCTGCTGATGCTCTGCAACGTGAAGGCTCGC CACTGCGGCGCGCAGCGGCACCACTTCGTCTTTAAGCATGACGCCTGGTTCATCGCCTTCATGGCTGCCT TTGCCTTCTCCAATGGCTACCTCGCCAGCCTCTGCATGTGCTTCGGGCCCAAGAAAGTCAAACCAGCTGA GGCGGAGACAGCAGGAAACATCATGTCCTTCTTTCTGTGTCTGGGCCTGGCTCTGGGAGCTGTGTTGTCC TTCTTGTTAAGGGCACTTGTGTGACCCTGTGGGGACAGAAGAACTACACTGCCTGCTTCCTGCTCACTTC CTTCCCTGCCAGGGACGAGCAGGGGTCGAGAGGGGCTGTTCTTCTAGCTGACTTCTGCTTTCCTCTGGAC TGTGCTTCGCCCAGCTGTCCAGGAGCCAGCGATGGCCTGCGGGTGGACTTGGAATTCAGGGTCAGAATGG CAAGGGCTCAATGGCCTCTGACTGACAGCTCCGACTGATGCCCGCTTACTCCAAGCACAAGAGACTCCAG GGCCAAGAGAGATCTGTCCGCCTGCCTATCACAGGATAGGGCGGAGGCGGATGGCTGATTGGTGTCGTGT GACCTGATGTCCCTCCCCTTGCCCTTCTTCCTTCTGTGCCTGTTCCATGTCCCCAGCCCTTGTCATTTTA CTGCCTTTTTTATACTGACAGAAACCAGGTGCCTTCAGAGGCCATCTGATTAAATAAACATTTTTTTTCT CCATAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_022880
• Insert Size: 1377 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC006812, AAH06812
• RefSeq Size: 1979 bp
• RefSeq ORF: 1377 bp
• Locus ID: 63959
• UniProt ID: Q9JIM1
• Cytogenetics: 17 B3- C
• Gene Summary: Mediates both influx and efflux of nucleosides across the membrane (equilibrative transporter). It is sensitive (ES) to low concentrations of the inhibitor nitrobenzylmercaptopurine riboside (NBMPR) and is sodium-independent. It has a higher affinity for adenosine. Resistant to dipyridamole and dilazep inhibition (anticancer chemotherapeutics drugs).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR and uses an alternate in-frame splice site in the coding region, compared to variant 1. It encodes an isoform (2) that is two amino acids shorter than isoform 1. Variants 2, 4, and 5 encode the same protein.