Spef1 (NM_027641) Mouse Untagged Clone

CAT#: MC200500

Spef1 (untagged) - Mouse sperm flagellar 1 (Spef1), (10ug)


  "NM_027641" in other vectors (4)

Reconstitution Protocol

USD 300.00

In Stock*

Size
    • 10 ug

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Frequently bought together (3)
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Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Symbol Spef1
Synonyms 4931426K16Rik; Clamp
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>BC006046 sequence for NM_027641
GCGAGAGGAGGCATTTGAAAGGTCCTGGGGCTGGCGCTGGGCTCTGTCGGGAACGTTGAGGCCTTGCTCT GGATCCAATGGAAAGCAGCGTGGATGAGGAGGCTCTGCACCAGCTGTACCTCTGGGTAGACAATATCCCT CTGTCCAGACCCAAGAGAAACCTCTCCCGGGACTTCAGTGACGGAGTCCTGGTTGCAGAGCTTATCAAGT TTTACTTCCCCAAGATGGTGGAGATGCACAATTATGTCCCTGCCAACTCTCTCCAGCAGAAGCTCAGCAA CTGGGGTCACCTGAACAGAAAGGTGCTGAACAAGCTGAACTTCTCGGTCCCTGACGATGTGATGCGGAAG ATTGCGCAGTGTTCTCCGGGAGTAGTGGAGCTAGTGCTCATTCCACTGAGGCAGCGCCTGGAGGAGCGGC AGAGGCGCCAGAAGCTGGGTGTTGGCTCCTTACAGGAGCTGGCTCCTCAGGATAGCAGTGGCTACATGGA TATGGGTTTGCCCCAGAAGGTTCGAGGAGAGGGTGCCCCAGCCTTGGGAGAGCAGCTCAGAGAGGGCCGT CCGTTGGCTTCCCGACCCCCTGGGTATAACCAGGCGCTGCAGGGTGATCCGAGCTTCGTCCTCCAGATCG CTGAAAAGGAGCAGGAGCTGTTGGCCTCTCAAGAGACAGTACAGGTCCTTCAGATGAAGGTGAAGCGTCT GGAACATCTGCTCCAACTTAAGAACGTGCGCATCGATGATCTCTCCCGGCGGCTCCAGCAGGCCGAGCGC AAGCAGCGGTGAGCGGTGCCAGGGCTGCGCCGGGCTTCCCAGTACCCGCCCGAGCCCAGACGCCGCGACC CACCCACTCACCTGCCGACGCAGGGTTGGGAGGGCGGAGCGAGCTGCTCACTAGTGCCTCCTGCAGCTAC GGCGCTCGGCTGCGGAGGGTTCGAGCGAACGTGGGGTTGCTCAACGCCGAGGCCGGGGACTGATCCTCCT CCTTACACTCCATCTGGGCCAGGAAAAAGGATCACTTTCCACAGCCCAGAAAGCCTGTACATCGCATGAT CTCCCAAACCACTTTGAAGCTCCCGGCGCTGGGCCTGGAGAGGCGGTCCTCTGTCTCGTTCCACTCCTCT AGAACCGGTTCCTTTCTCAGCATAGAAGATAGCTTCATCTCTATCCTTGCTAAATCTCCAAGGCCTCCGA CACTCGAGAAATTATCCCGGGAAGCTGAGGAATTCTCTTGCCCATCCTGAGTGCTGACCGGGGACCAGTT TTAGGGCTGGAAATCACAGCAGCTGGAGAGGAAGGGATAAGGTGTTGAAGTAGGCGGGAGTAAGGGAGGC ACCCAGCTGTGCAGTCGCTCTCCTCTCACGGAGCATTCTAAGGAGTGCCTGGGAAGATAGGGCAGAGTTG TCTCCATTAAAGGTGCTGTTGTGCGTTAAAAAAAAAAAAAAAA
Restriction Sites RsrII-NotI     
ACCN NM_027641
Insert Size 705 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq BC006046, AAH06046
RefSeq Size 1443 bp
RefSeq ORF 705 bp
Locus ID 70997
UniProt ID Q99JL1
Cytogenetics 2
Gene Summary Microtubule-associated protein that promotes microtubule bundling and stabilizes microtubules against depolymerization in response to cold shock (PubMed:16206169). Microtubule-associated protein involved in the stabilization of microtubules along the axis of migration during radial intercalation. Promotes the establishment and stabilization of an axis of microtubules required for the active migration of cells into the outer epithelium (By similarity).[UniProtKB/Swiss-Prot Function]

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