Eef2 (NM_007907) Mouse Untagged Clone

CAT#: MC200488

Eef2 (untagged) - Mouse eukaryotic translation elongation factor 2 (Eef2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Eef2
• Synonyms: Ef-2
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC007152 sequence for NM_007907
  CGGACGCGTGGGGCCGCCGCCATCGTCGGCGCGCTTCCCTGTTCACCTCTGACTCTGAGAATCCGTCGCC ATCCGCCACCATGGTGAACTTCACAGTAGATCAGATCCGTGCCATCATGGACAAGAAAGCCAACATCCGG AACATGTCAGTCATCGCCCATGTGGACCACGGCAAGTCCACGCTGACCGACTCCCTTGTGTGCAAGGCTG GCATCATTGCCTCTGCCCGAGCTGGGGAGACGCGCTTCACTGACACTCGCAAGGATGAGCAGGAGCGCTG CATCACAATCAAATCCACCGCCATCTCCCTCTTCTACGAGCTCTCTGAGAACGACCTGAACTTCATTAAG CAGAGCAAGGATGGCTCGGGCTTCCTCATCAACCTCATCGACTCTCCAGGCCATGTGGACTTCTCTTCAG AGGTGACAGCTGCCTTGCGTGTCACCGATGGAGCTCTGGTGGTGGTGGACTGTGTGTCTGGCGTGTGTGT GCAGACAGAAACTGTGCTGCGCCAGGCCATCGCTGAACGCATCAAGCCCGTCCTGATGATGAACAAGATG GACCGGGCCCTGCTGGAGCTGCAGCTGGAGCCCGAGGAGCTCTACCAGACCTTCCAGCGCATCGTGGAGA ACGTCAACGTCATCATCTCTACCTACGGCGAGGGCGAGAGTGGGCCCATGGGCAATATCATGATTGACCC CGTCCTGGGCACCGTAGGCTTTGGTTCTGGCCTGCATGGCTGGGCCTTCACCCTGAAGCAGTTTGCGGAG ATGTATGTGGCCAAGTTTGCAGCCAAGGGTGAGGGCCAGCTGAGCGCAGCCGAGCGTGCCAAGAAAGTAG AGGACATGATGAAGAAGCTGTGGGGAGACCGGTACTTTGATCCGGCCAATGGCAAGTTCAGTAAGTCAGC CAATAGCCCAGATGGGAAAAAACTTCCCCGCACCTTCTGCCAGCTCATCCTGGACCCCATCTTCAAGGTG TTCGACGCCATCATGAACTTCAGGAAGGAGGAGACAGCCAAGCTGATCGAGAAGCTGGACATCAAGCTGG ACAGCGAGGACAAAGACAAGGAGGGCAAGCCATTGCTCAAGGCTGTGATGCGCCGCTGGCTGCCTGCAGG GGACGCCCTCCTGCAGATGATCACCATCCACTTACCATCCCCCGTCACTGCACAGAAGTACCGTTGTGAG CTGCTATACGAGGGGCCTCCCGACGATGAGGCCGCCATGGGTATTAAGAGCTGCGACCCCAAAGGCCCAC TTATGATGTACATTTCCAAGATGGTGCCAACCTCTGACAAAGGCCGCTTCTATGCCTTTGGTAGAGTGTT CTCTGGGGTGGTGTCCACGGGCCTGAAGGTCCGGATCATGGGCCCCAACTACACGCCTGGGAAGAAAGAG GACCTATACCTGAAGCCTATCCAGAGAACCATTCTGATGATGGGCCGCTACGTGGAGCCGATTGAGGACG TGCCATGTGGAAACATTGTGGGGCTGGTCGGAGTGGACCAGTTCCTTGTGAAGACGGGGACCATCACTAC CTTTGAGCACGCTCACAACATGCGCGTGATGAAGTTCAGCGTCAGCCCTGTCGTCCGTGTGGCAGTGGAG GCCAAGAACCCAGCTGACCTGCCCAAGCTGGTGGAGGGGCTGAAGCGGCTGGCTAAGTCTGACCCTATGG TGCAGTGCATCATTGAGGAGTCTGGAGAACATATTATTGCTGGCGCCGGTGAGCTGCACCTGGAGATCTG CCTTAAGGACCTGGAGGAGGACCATGCCTGCATCCCCATCAAGAAATCTGACCCTGTTGTGTCATATCGG GAGACAGTCAGTGAGGAATCCAACGTGCTGTGTCTGTCCAAGTCCCCCAATAAGCACAACCGGCTGTACA TGAAGGCCAGGCCCTTCCCTGATGGCCTGGCAGAGGACATCGATAAGGGTGAGGTGTCTGCCCGCCAGGA GCTCAAGGCACGTGCCCGCTACCTGGCCGAAAAGTATGAGTGGGACGTTGCTGAAGCCCGCAAGATCTGG TGCTTTGGCCCTGATGGCACTGGCCCCAACATTCTCACCGACATCACCAAGGGTGTGCAGTACCTGAATG AGATCAAGGACAGTGTGGTGGCTGGCTTCCAGTGGGCTACTAAGGAGGGCGCTCTCTGTGAGGAAAACAT GCGTGGTGTGCGGTTTGATGTTCATGATGTGACCCTGCATGCTGATGCCATTCACCGGGGAGGTGGCCAG ATCATCCCCACAGCACGCCGCTGCCTGTATGCCAGTGTGCTGACCGCACAGCCCCGCCTCATGGAGCCTA TCTATCTGGTGGAGATCCAGTGTCCTGAGCAAGTGGTGGGTGGCATCTACGGTGTCCTGAACAGGAAGCG TGGCCATGTGTTTGAGGAGTCCCAGGTGGCTGGTACCCCCATGTTTGTGGTCAAGGCATACCTGCCTGTC AATGAGTCCTTTGGCTTCACCGCTGATCTGCGATCCAACACCGGCGGCCAGGCCTTCCCCCAGTGCGTGT TTGACCACTGGCAGATCCTGCCTGGGGATCCTTTTGACAACAGCAGCCGCCCCAGCCAAGTGGTAGCTGA GACGCGCAAGCGCAAGGGCCTGAAAGAGGGCATCCCAGCGCTGGACAACTTCCTGGACAAACTGTAGGCA GCCTGATACTGCCACATGCTGCACAGTGCCCACCCATCAGAAGACACCTTGAGACTGTCCCACAGTGCTC CTCTAGAGGCTGCTGGGGCCACCCTGACATCACTCAGCACTCACTTGCTACCAATTCTATTTATTTCGGA ATTACAAGATAGCGGGAATCTCTCTGCAGGCTGGACTGGCAGGCTGTGGGGTGGGCGGGACACGGCTCTT AACATTTTCAGAGGGAAACGCGCAGATGTCCAAAAGTCTAAATAAATGCATTCAGAGGTTTTTGGGGTCC ATGGCCAAGTGGAGTTCCCCCAGAGGGGGAGGTGGGGTAAGTGCCTCCAGGAAGGCAGGCAGCCTGCCTT AGACTTGCAGCCCGGCTGTGGGAATGAATCATTGGAGTAATAAACTACAGTGGTTGATCCAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_007907
• Insert Size: 2577 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC007152, AAH07152
• RefSeq Size: 3111 bp
• RefSeq ORF: 2577 bp
• Locus ID: 13629
• UniProt ID: P58252
• Cytogenetics: 10 C1
• Gene Summary: Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.[UniProtKB/Swiss-Prot Function]