Eef2k (NM_007908) Mouse Untagged Clone

CAT#: MC200428

Eef2k (untagged) - Mouse eukaryotic elongation factor-2 kinase (Eef2k), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Eef2k
• Synonyms: C86191; eEF-2K
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC003433 sequence for NM_007908
  GGCAGCAGCCCCAACTCCAGCCGGTGCTCGGCTCCAGCGCTCCGACGTCTTCAGGCGCCCCACTGCGGCT GCGGGCCTGGCTCTCTGTCGCCTGCGCTTTGTACCGGGGATTCTCCTCCCTCCCTGCACCCTGGAGCGCC CGGCCGCCAGCCTTTGCCAGCTCCTTGGGATCCTTGCGGCCGTGGGCAGCAGCTGTCGCGCCTGCCACAC TGTGGGAGGACCATCCTTGCTGCACGTGCGCAGTAACTCTGGCTGCACAGCGGAGCCCCCGGATAACACG GTCGCCCCCGCAACATGGCAGACGAAGACCTCATCTTCTGCCTGGAAGGTGTTGACGGTGGCAGGTGCTC CCGAGCTGGCCACAATGCGGACTCTGACACAGACAGTGACGATGATGAGGGCTATTTCATCTGCCCCATC ACTGATGACCACATGTCCAATCAGAATGTCAGCTCCAAAGTCCAGAGCTACTATAGCAACCTAACAAAAA CAGAGTGCGGCTCCACAGGGTCACCAGCCAGCTCCTTCCACTTCAAGGAAGCCTGGAAGCATGCGATCGA GAAAGCCAAGCACATGCCTGACCCCTGGGCTGAATTCCATCTCGAGGACATCGCCACAGAACATGCTACT CGGCACAGGTACAACGCTGTCACCGGGGAATGGCTGAAAGACGAGGTTCTGATCAAGATGGCGTCTCAGC CCTTCGGCCGTGGAGCAATGAGGGAGTGCTTCAGGACGAAGAAACTCTCCAACTTCTTGCACGCCCAGCA ATGGAAGGGGGCCTCCAACTACGTGGCCAAGCGCTACATCGAGCCGGTGGACAGGAGCGTGTACTTTGAG GATGTGCAGCTCCAGATGGAGGCGAAGCTCTGGGGGGAGGATTACAATCGGCACAAGCCCCCCAAGCAGG TGGATATCATGCAGATGTGCATCATTGAGCTAAAGGACAGACCAGGCCAGCCCCTCTTCCACTTGGAGCA CTACATTGAGGGCAAGTACATCAAGTACAATTCCAACTCAGGCTTTGTCCGTGATGACAACATCCGACTA ACCCCACAGGCCTTCAGCCATTTCACATTTGAGCGTTCTGGTCATCAGCTGATTGTAGTGGACATCCAGG GTGTGGGTGACCTTTATACCGACCCACAGATCCACACTGAGAAAGGCACTGACTTTGGAGATGGTAACCT TGGTGTCCGGGGAATGGCTCTCTTCTTCTACTCTCATGCCTGCAACCGGATTTGTCAGAGCATGGGCCTT ACGCCCTTTGACCTCTCCCCACGGGAACAGGATGCGGTGAATCAGAGCACCAGGCTATTGCAATCAGCCA AGACCATCTTGAGGGGGACAGAGGAGAAGTGTGGGAGTCCCCGCATAAGGACACTCTCTAGCAGCCGGCC CCCTTTGCTCCTTCGCCTGTCAGAGAACTCCGGGGATGAGAACATGAGTGACGTGACCTTTGACTCTCTG CCTTCCTCCCCGTCTTCAGCTACACCACACAGCCAGAAACTGGACCACCTCCATTGGCCAGTGTTTGGTG ACCTCGATAACATGGGCCCTAGAGACCATGACCGCATGGACAATCACCGGGACTCTGAGAATAGTGGGGA CAGTGGGTATCCAAGCGAGAAGCGAAGTGACCTGGATGATCCTGAGCCCCGAGAACACGGCCACTCCAAC GGCAACCGAAGGCATGAATCTGACGAGGATAGCCTGGGCAGCTCTGGACGGGTCTGTGTGGAGACGTGGA ACCTGCTCAATCCCTCCCGCCTGCACCTGCCGAGGCCCTCGGCCGTGGCCCTAGAAGTGCAGAGGCTAAA TGCCCTGGACCTTGGAAGGAAAATCGGGAAGTCTGTTTTGGGGAAGGTCCATCTGGCCATGGTGCGATAC CACGAGGGCGGGCGCTTCTGCGAGAAGGATGAGGAGTGGGATCGAGAGTCAGCCATCTTCCATCTGGAGC ATGCAGCTGACCTGGGAGAACTGGAGGCCATCGTGGGCCTAGGCCTCATGTACTCTCAGCTGCCCCACCA CATCCTGGCTGATGTCTCTCTGAAGGAGACAGAGGAGAACAAGACAAAAGGCTTTGATTACTTACTGAAG GCGGCAGAAGCTGGTGACAGGCATTCCATGATTTTAGTGGCCCGAGCTTTTGACACTGGCCTGAACCTCA GCCCAGACAGGTGTCAAGACTGGTCGGAAGCCTTGCACTGGTACAACACAGCCCTGGAGACAACAGACTG CGATGAAGGCGGGGAGTACGATGGGATACAGGACGAGCCCCAGTACGCACTGCTGGCCAGGGAGGCGGAG ATGCTGCTCACCGGGGGATTTGGACTGGACAAGAACCCCCAAAGATCAGGAGATTTGTACACCCAGGCAG CTGAGGCAGCAATGGAAGCCATGAAGGGCCGGCTAGCCAACCAGTACTACGAGAAGGCGGAAGAGGCCTG GGCCCAGATGGAGGAATAACTGCCCCACAGAATCACTGCCAAGGAGAAAGGCTGGGGAAGGGAGGATGGG ACTCATTTCTAGGGAGGGGAGAAGCAGTTTTATTATGGTATAAACTCACTTTTGTATTCCGTTTTCTCAC TCTTGATAGCCTTTGCAGCTGGCCGAGACATTATAACTGCCCTTTTAGTGGTGGTACTTTGGGGGAAGTG GGGGGTTGAAGATGCAGCCTAATGAGTCTCTTCCCCGTCCTCCACCCCCCGGTTTTCCCTTTTTCATCCT GAGATGTAAGCAAGTATCTTTCTGTCTGGATGATCAGACAGTATCAGGGCTTCTCTCGCCAGCCTTTGCA GGCAGGACTCACATCTCAGGGTCTCCAGGCCATGTGCTGGGAAACCTTTCTATTGGCTTTCCACAATGGG TCTTTCTTTCAGTTTTGAGAGAGGTATGTTGTTTGTTTGTTTGTTTCCTCCAGTGGATGTGAGATGGTTC AATACCCAGGCTCCTTCATGCTGGGGGAGATAGTTGTATAACTCTTCCACTTAATGCCCTTCCACTGGAC ATGTGATTCCTGGCATCCTTCTAAATTTTTTTTTTTTAGACCTGCTATGTAATATAGATTTTTCAAAAAC AAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_007908
• Insert Size: 2175 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC003433, AAH03433
• RefSeq Size: 3095 bp
• RefSeq ORF: 2175 bp
• Locus ID: 13631
• UniProt ID: O08796
• Cytogenetics: 7 F2
• Gene Summary: Threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. Upon activation by a variety of upstream kinases including AMPK or TRPM7, phosphorylates the elongation factor EEF2 at a single site, renders it unable to bind ribosomes and thus inactive. In turn, the rate of protein synthesis is reduced.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the functional protein. Variants 1, 2 and 3 encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.