Wars (NM_011710) Mouse Untagged Clone

CAT#: MC200422

Wars (untagged) - Mouse tryptophanyl-tRNA synthetase (Wars), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Wars
• Synonyms: TrpRS; WRS
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC003450 sequence for NM_011710
  GCTGTCCAGCTTGAGCACCCTTGGACTACACAGCCAGGAGTCCTGTCTCAACTGCTGAACACACTACTCA CAAGCCAGCTGCAGGCCTAGGTCCTAGAAGATGGCAGACATGCCCAGTGGCGAGTCCTGCACCTCTCCAC TGGAGCTGTTCAACAGCATAGCTACTCAAGGGGAGCTCGTGAGGTCCCTCAAAGCTGGAAATGCACCAAA AGATGAGATTGATTCTGCAGTAAAGATGTTGTTATCATTAAAAGTGAGCTATAAGGCCGCCATGGGGGAG GAGTACAAGGCTGGCTGCCCTCCTGGGAACCCAACAGCTGGGAGAAATTGTGACTCTGATGCCACAAAAG CCAGCGAGGATTTTGTGGATCCATGGACAGTGCGGACAAGCAGTGCAAAAGGCATCGACTATGACAAGCT CATTGTTCAGTTTGGAAGCAGCAAAATTGACAAAGAGCTGATAAACCGAATAGAGCGAGCCACTGGCCAA AGACCGCACCGCTTCCTGCGCCGGGGCATCTTCTTCTCCCACCGAGATATGAATCAAATTCTGGATGCCT ATGAAAATAAGAAGCCATTCTACCTGTACACAGGCAGGGGCCCCTCCTCCGAAGCAATGCATCTGGGTCA TCTCGTCCCGTTTATTTTCACAAAGTGGCTACAGGATGTGTTCAATGTGCCTTTGGTCATCCAGATGTCT GATGACGAGAAGTACCTGTGGAAGGACTTGACTCTGGAGCAGGCATATAGCTACACCGTAGAAAATGCAA AGGACATCATTGCCTGTGGCTTCGACATCAACAAAACTTTCATCTTCTCTGATCTTGAGTATATGGGGCA GAGCCCGGGCTTCTACAGGAACGTGGTGAAGATTCAGAAGCACGTCACCTTCAACCAAGTGAAAGGCATT TTCGGCTTTACGGACAGTGACTGCATCGGGAAGATCAGTTTTCCTGCTGTCCAGGCCGCGCCATCGTTCA GCAACTCTTTCCCAAAGATCTTCCGAGACAGGACAGATATCCAGTGCCTCATCCCGTGTGCCATTGACCA GGATCCGTACTTCAGAATGACAAGGGACGTGGCCCCCAGGATCGGCCATCCTAAACCTGCCCTGCTGCAC TCGACCTTCTTCCCTGCCCTGCAGGGTGCTCAGACCAAGATGAGTGCCAGTGACCCCAACTCCTCCATCT TCCTCACTGACACGGCCAAGCAGATCAAGAGCAAGGTCAACAAGCATGCATTTTCTGGAGGAAGAGACAC TGTGGAGGAGCACAGGCAGTTTGGGGGCAACTGTGAAGTGGATGTATCCTTCATGTATCTGACCTTCTTC CTTGAAGATGACGACAGGCTGGAGCAGATCAGAAAGGATTACACCAGCGGAGCCATGCTCACTGGGGAGC TCAAGAAGACGCTCATAGACGTCCTTCAGCCCCTGATCGCAGAGCACCAGGCCCGACGCAAGGCGGTCAC CGAGGAGACCGTGAAGGAGTTCATGACGCCCAGGCAGCTGTCCTTCCACTTCCAGTGCTTCTGCTTTGAC ACTTGACTGACGGGGAGGGCAGGCCAGAGCAGTGTGCTGGCCATGCCTCAGGCCGTTGACCATAGCTAGA CAGAAAAGAACAGCAGCCGTCCAGGCCGCAGCGTTCTCCCTCTGGGCGGGCTGCATGGAATGAGCCTCCA CTCAGAGTCTTGGAAAAGCAGAAGCCGTACCTCAGGGAAAGCCCCTGGTTGAAGTGTCTGTCCTCTCTGA AGGCACAAGGGCCACTCTGAGTGTTCTGTCCTTGCATCATTTTTAGGAAAAAACAATAAACTTTGTTGAG CTCCAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_011710
• Insert Size: 1446 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC003450, AAH03450
• RefSeq Size: 1838 bp
• RefSeq ORF: 1446 bp
• Locus ID: 22375
• UniProt ID: P32921
• Cytogenetics: 12 F1
• Gene Summary: T1-TrpRS has aminoacylation activity while T2-TrpRS lacks it. T1-TrpRS and T2-TrpRS possess angiostatic activity. T2-TrpRS inhibits fluid shear stress-activated responses of endothelial cells. Regulates ERK, Akt, and eNOS activation pathways that are associated with angiogenesis, cytoskeletal reorganization and shear stress-responsive gene expression (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.