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Fbxl12 (NM_001002846) Mouse Untagged Clone

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SKU
MC200397
Fbxl12 (untagged) - Mouse F-box and leucine-rich repeat protein 12 (Fbxl12), transcript variant 2, (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK DDK
AAV Particle:
DDK DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Fbxl12
Synonyms 3110048D16Rik; Fbl12
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC005699 sequence for NM_001002846
GCAAAAGAAACTGTTTAGCTCTAGGAAATGAAGCTCAGAGAGGAGCAGAGTCCCAGGGAGACACCAGAAC CCAGGTCCCAGGCGTGTCTGTCACCAGCTAGATGGATCATCTGGGAGCCCCCGTAGCTCTCTCTCAGTCA CCCCAATCTCTGGCTCTTACAGATGCGGCCGAAAGTCATGTGGCACCTCCTGCGCCGGTACATGGCGTCC CGGCTCTACTCGTTGCGCATGGGCGGCTACCTGTTTTCTGGCTCTCAGGCCCCACAGCTGTCCCCCGCCT TGATGAGGGCCCTGGGTCAGAAATGCCCCAATCTGAAGCGCCTGTGCCTGCACGTGGCTGACCTGAGCAT GGTGCCTATCACCAGCCTTCCTAGCACACTGAGGACCCTGGAGCTGCACAGCTGCGAAATCTCCATGATC TGGTTGCAGAAAGAGCAGGACCCCACGGTGCTGCCTCTTCTGGAATGCATCGTGCTGGACCGAGTGCCGG CCTTCCGCGATGAGCATCTGCAGGGCCTTACCCGATTCCGAGCCCTGCGCTCGCTGGTGCTGGGCGGCAC CTACCGGGTCACTGAGACCGGGCTAGATGCCAGCCTGCAGGAGCTCAGCTACCTGCAAAGGCTTGAGGTG CTGGGCTGCACCCTGTCAGCTGACAGCACGCTGCTGGCCATCAGCCGCCACCTTCGAGATGTGCGCAAGA TTCGGCTGACCGTTGGGGGCCTCTCAGCCCAGGGCCTGGTCTTCCTGGAGGGAATGCCTGTCCTGGAGAG TTTGTGCTTCCAGGGTCCCCTTATCACCCCAGACATGCCCACACCCACTCAGATTGTGTCCTCCTGCCTC ACCATGCCGAAGCTCAGAGTGCTTGAGGTGCAAGGGCTGGGCTGGGAGGGTCAGGAAGCAGAGAAAATCC TGTGCAAGGGCCTGCCCCACTGCATAGTTATTGTTAGGGCTTGTCCCAAAGAATCCATGGATTGGTGGAT GTGAGGGCATCACCCATCCATCTCTGGAACCTAGCCTAGCTTGGAGAGTGACCTTATAAGCAAACTTAAG CATGTAAATTCATCAGGCGTGAGGGAACTGGAGGTGTCAAGGAAAGGAAACTAGGACCTTGGGGCTTCCT GAGCTGGGGTTATCTGTAATCTGGGTCACCTCAGACTTGTCATCAAGCCTCTGGAAGACCCCGTTCACAC ATTTAGGAATAGGCATCATAGCTACCTCATGATTCATTTTAAAATCTTTAACACCCCCTTCCCCTCTTTC TTTTCCAGTACCTGGGACTCAAACCCATGGCCTTAGGTCTGGTAGAGAAGCACTGCTGTGCTGCTGAGCC ACCCCAGAGACCTTAGAGAGGGCTCATTTTCAAGTGGAGGTGTGTGGAGTTCAGGGCTCTGAGGTTGGCC TGGTTTTCTACATACATGGTTGAGAGGCATAGAGGGCAGGGGCAAGGGAGCATGCTGTTGGATTGTCCTA GTTTTCTAGAAGGTTCTCAGTCATATTTACTGAATTTTGTATCCTGTCTTTTTCAAGTTTTCCACAGCAT ATATTTTACTATTAAAGAAAAATAATGTTTTTCAAAAAAAAAAAAAAA
Restriction Sites RsrII-NotI |
ACCN NM_001002846
Insert Size 822 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC005699, AAH05699
RefSeq Size 1588 bp
RefSeq ORF 822 bp
Locus ID 30843
UniProt ID Q9EPX5
Cytogenetics 9 A3
Summary Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. Mediates the polyubiquitination and proteasomal degradation of CAMK1 leading to disruption of cyclin D1/CDK4 complex assembly which results in G1 cell cycle arrest in lung epithelia (By similarity).UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. It encodes isoform b, which has a shorter N-terminus, compared to isoform a. Variants 2 and 4 encode the same protein.
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Documents
Resources
"NM_001002846" in other vectors (5)
SKU Description Size Price
MG203656 Fbxl12 (tGFP-tagged) - Mouse F-box and leucine-rich repeat protein 12 (Fbxl12), transcript variant 2 10 ug
$500.00
MR203656 Fbxl12 (Myc-DDK-tagged) - Mouse F-box and leucine-rich repeat protein 12 (Fbxl12), transcript variant 2 10 ug
$289.00 $300.00
MR203656L3 Lenti ORF clone of Fbxl12 (Myc-DDK-tagged) - Mouse F-box and leucine-rich repeat protein 12 (Fbxl12), transcript variant 2 10 ug
$600.00
MR203656L4 Lenti ORF clone of Fbxl12 (mGFP-tagged) - Mouse F-box and leucine-rich repeat protein 12 (Fbxl12), transcript variant 2 10 ug
$600.00
MR204773 Fbxl12 (Myc-DDK-tagged) - Mouse F-box and leucine-rich repeat protein 12 (Fbxl12), transcript variant 2 10 ug
$289.00 $300.00

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