C1qa (BC002086) Mouse Untagged Clone

CAT#: MC200379

C1qa (untagged) - Mouse complement component 1, q subcomponent, alpha polypeptide (cDNA clone MGC:6262 IMAGE:3592169), complete, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: C1qa
• Synonyms: AI255395; C1q
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC002086
  GGTGTCCCACCATCAGCAAAGGGCTGGAGCATCCAGTTTGATCGGACCACGGAGGCAGGGACACCATGGA GACCTCTCAGGGATGGCTGGTGGCCTGTGTGCTGACCATGACCCTAGTATGGACAGTGGCTGAAGATGTC TGCCGAGCACCCAACGGGAAGGATGGGGCTCCAGGAAATCCTGGCCGCCCGGGGAGGCCGGGTCTCAAAG GAGAGAGAGGGGAGCCAGGAGCTGCTGGCATCCGGACTGGTATCCGAGGTTTTAAAGGAGACCCAGGGGA ATCTGGCCCCCCTGGCAAACCTGGCAATGTGGGGCTCCCAGGTCCCAGTGGTCCCCTGGGGGACAGCGGC CCCCAAGGACTGAAGGGCGTGAAAGGCAATCCAGGCAATATCAGGGACCAGCCCCGGCCAGCTTTCTCAG CCATTCGGCAGAACCCAATGACGCTTGGCAACGTGGTTATCTTTGACAAGGTCCTCACCAACCAGGAGAG TCCATACCAGAACCACACGGGTCGCTTCATCTGTGCAGTGCCCGGCTTCTATTACTTCAACTTCCAAGTG ATCTCCAAGTGGGACCTTTGTCTGTTTATCAAGTCTTCCTCCGGGGGCCAGCCCAGGGATTCCCTGAGTT TCTCTAACACCAACAACAAGGGGCTCTTTCAGGTGTTAGCAGGGGGCACCGTGCTTCAGCTGCGACGAGG GGACGAGGTGTGGATCGAAAAGGACCCCGCAAAGGGTCGCATTTACCAGGGCACTGAAGCCGACAGCATC TTCAGCGGATTCCTCATTTTCCCCTCGGCCTGAGCTGGGGATCTGCCCCTGCATCCTGCCATCTCCTGCG CTCCCTGTTGTGGACCACGCCCCCCCCCTCCGTCCTGCACCCCTCCCCTCCGCACTCTGCAAAGTGAAGG GGCTGGGGCTTTAGCACCCTGGGGGAGGGGCTGTCTCGGAGGGAGGGCACTGAGGACTGATGTTTCTGCA CACTGCCCAGTGGTTTCTTTAAGCACTTTCTGGAATAAACGATCCCCGTCTGTGTCTGTGAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: BC002086
• Insert Size: 738 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC002086, AAH02086
• RefSeq Size: 1080 bp
• RefSeq ORF: 738 bp
• Locus ID: 12259
• Cytogenetics: 4 69.05 cM
• Gene Summary: C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2+)-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes.[UniProtKB/Swiss-Prot Function]