Sgta (NM_024499) Mouse Untagged Clone

CAT#: MC200304

Sgta (untagged) - Mouse small glutamine-rich tetratricopeptide repeat (TPR)-containing, alpha (Sgta), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Sgta
• Synonyms: 5330427H01Rik; AI194281; D10Ertd190e; Sgt; Stg
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC003836 sequence for NM_024499
  TGGGTCGGGTCTTCGGTCGACTGAGAGATCCTGAGCAAGCAGTGTCACCTCACTAGTCTCGAGATGGACA ACAGGAAGCGCCTGGCCTATGCCATCATCCAGTTCCTCCATGGCCAGTTGCGGCACGGTGGGCTCTCGTG TGACGCCCAGGAGAGCCTAGAGGTTGCCATCCAGTGTCTGGAGACAGCCTTCGGGGTGACGCTGGAGGAC AGTGACCTTGCTCTCCCCCAGACCCTGCCAGAGATATTTGAAGCAGCCACTTCCAGCAAGCAGGAGATGC CACAGGACCCGCGGGCCCCTGACAGGACACCACCCTCTGAAGAGGACTCAGCTGAGGCAGAGCGCCTCAA AACGGAAGGTAATGAGCAGATGAAGCTGGAGAACTTCGAGGCGGCTGTGCACCTCTACGGCAAGGCCATT GAGCTCAACCCTGCCAATGCTGTCTACTTCTGTAACAGAGCTGCGGCCTACAGCAAGCTGGGGAACTACG TGGGGGCCGTGCAGGACTGCGAGCGCGCCATTGGCATCGACCCTGGGTATAGCAAGGCCTATGGCCGCAT GGGCCTTGCACTATCCAGCCTGAACAAGCATGCAGAGGCTGTGGCATACTACAAGAAGGCCCTGGAGCTG GACCCTGACAACGACACATACAAGTCCAACCTCAAGATTGCAGAGCTGAAGTTACGGGAGGCGCCAAGCC CTACGGGCGGCGTGGGCAGCTTAGACATCGCTGGCCTGCTGAACAACCCACACTTCATCACCATGGCTTC GAGCTTAATGAACAGCCCCCAGCTACAGCAGCTCATGTCGGGCATGATCTCCGGAGGCCATAACCCTCTG GGCACGCCAGGCAGCAGTCCGCAGCAGAGTGACCTGGCCAGCCTCATCCAGGCGGGCCAACAGTTTGCAC AGCAGATGCAGCAGCAGAACCCAGAGTTTGTGGAGCAGATCCGGAGCCAGGTGGTGCGCAGCCGGACACC CAGTGCCAGCCATGAGGAGCAGCAAGAGTGACAACGGTGACCCGAGTGTCCCTTCGCTCCGCGGCCTCCT GGTGTTTTAGCATTTTCTCCGTCGGGCAGCTCGAGAGAGAAACTGGACCTGCATGTTAAGACGGACTCGT CCCCACCTTGTGTTTTCCTTCATCCCTCCCCTTCGGTTTTGTACTCTTACTGGCCCCACACCTCCCTGGA GGAGTCTACAGAAGACGACACACCAGCACCAGTTCCTCCAGCCACCTAGGAGCTGCAGGGAGGGGTGGGG TGAGTGTGAGTCACTGAGTGACAGCCTGGGCCCTCAGGTCTCAGGCCCCACCCTGCCGGGTTCCTCCTAA AGGGACTTGAGTAGCTGACTCTCATCCCCACTGCCAGCCCCAGCTGGGCACCAGCGTCCCACACCCTCGC CTGGTTGCAGGTCCCAGCAGGTGCCCCCGCCTGCCTTATTCCAGTGGCCTGTGCCACATAGGACTGAGGC CTGTGACTTCTGCCCAGCGTGTGGACCATCCGTGTCTCTTTCCAGCTTCCCCTCCATCTTTACAGCCATC AGCCAATCCTAACTTTGTGTCCAGTCCAGCACCATCCTCAGCCTGGGCAGCAGACATAGACATTGACCCA TCTGTGTCATCCTATCCTTGATCTCATGGGAATTCAATGTCTGGGGTTGGCAGGGAGTTTCGGGGTATCG GCCCTGAAGCAGCCACCAGAACCATCTTTGTTGGGGTCAGTCTGAGTGAGGGTGTGGATGTTCCCACACA CATTCTTGGCCCTAGGAGCAGGTGATGAGATCACAGGACAGCTGAACCCTTGGGGGGTCATGATGGCATC AGGCTTTGGCAGATAGTGTCAGAGCTGTGTGTGTGGTAGGTAGCCAGTAGGAAGATGGGCCCCCGAGGGC AGCAGCGCCACCTTGGGCACAGAGTGCCTGGAGAATAAAGACAGTGATGACGCTCTGAAAAAAAAAAAAA AAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_024499
• Insert Size: 948 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC003836, AAH03836
• RefSeq Size: 1966 bp
• RefSeq ORF: 948 bp
• Locus ID: 52551
• UniProt ID: Q8BJU0
• Cytogenetics: 10 39.72 cM
• Gene Summary: Co-chaperone that binds misfolded and hydrophobic patches-containing client proteins in the cytosol. Mediates their targeting to the endoplasmic reticulum but also regulates their sorting to the proteasome when targeting fails. Functions in tail-anchored/type II transmembrane proteins membrane insertion constituting with ASNA1 and the BAG6 complex a targeting module. Functions upstream of the BAG6 complex and ASNA1, binding more rapidly the transmembrane domain of newly synthesized proteins. It is also involved in the regulation of the endoplasmic reticulum-associated misfolded protein catabolic process via its interaction with BAG6: collaborates with the BAG6 complex to maintain hydrophobic substrates in non-ubiquitinated states. Competes with RNF126 for interaction with BAG6, preventing the ubiquitination of client proteins associated with the BAG6 complex. Binds directly to HSC70 and HSP70 and regulates their ATPase activity.[UniProtKB/Swiss-Prot Function]