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Ctsh (BC006878) Mouse Untagged Clone

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SKU
MC200296
Ctsh (untagged) - Mouse cathepsin H (cDNA clone MGC:6103 IMAGE:2647229), (10ug)
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  $457.00
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Ctsh
Synonyms AL022844
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC006878
GGGACCGGGCAGTGAGCGCCGAGATGTGGGCTGCGCTGCCGCTGCTGTGCGCTGGGGCCTGGCTGCTGAG TACTGGGGCCACCGCCGAGCTGACCGTGAACGCCATAGAAAAGTTTCACTTTAAGTCATGGATGAAACAG CATCAAAAGACGTACAGCTCGGTGGAGTACAACCACAGACTGCAGATGTTTGCCAACAACTGGAGGAAGA TTCAAGCCCACAACCAGAGGAACCACACATTTAAAATGGCATTGAACCAGTTTTCAGATATGAGCTTTGC TGAAATAAAACACAAATTCCTTTGGTCAGAGCCTCAGAATTGCTCAGCCACCAAAAGTAACTACCTCCGA GGTACAGGCCCCTACCCTTCCTCCATGGACTGGAGGAAGAAAGGAAATGTTGTTTCGCCAGTGATAAATC AGGGGGCCTGTGGCAGCTGCTGGACTTTCTCTACCACGGGGGCCCTAGAGTCAGCTGTGGCTATTGCCAG TGGGAAAATGCTGTCTTTGGCTGAGCAGCAGCTGGTGGATTGTGCCCAAGCCTTCAACAATCATGGCTGC AAAGGAGGTCTCCCCAGCCAGGCCTTCGAGTACATCCTATACAACAAGGGCATCATGGAAGAAGACAGCT ACCCTTACATAGGCAAGGATAGTTCATGCAGATTCAACCCCCAAAAAGCTGTTGCATTCGTCAAGAATGT TGTCAACATCACACTCAATGACGAGGCTGCAATGGTTGAGGCTGTGGCTCTATACAACCCTGTGAGCTTC GCCTTTGAGGTGACTGAAGATTTTTTGATGTATAAAAGTGGCGTCTACTCCAGTAAATCCTGTCATAAAA CTCCAGATAAAGTAAACCATGCAGTCCTGGCGGTTGGCTATGGAGAACAGAATGGATTACTCTACTGGAT TGTGAAAAACTCTTGGGGCTCCCAGTGGGGGGAGAATGGGTACTTCCTCATTGAACGTGGGAAGAACATG TGTGGCCTGGCTGCTTGTGCCTCCTATCCCATTCCTCAGGTATAAGCCACGGCTGCACGGGCAACTGATT GGCAGACCAAGGGAGGAACTGGTCCTTCGATGAGAATGCCACCCTGGAGAAAATTGTGTGGAAATCCACC CAGAGGCCCTCTCACTCCTGAGTCTAGACGCCTAAAGACAGGAAGGACAAACTTGAATAGCGACAAGCCC ACCCACGTGACATCATCACCAGAAATACGCTTGGATTGTGGTTTTTTAATGACCCAAACCCACGTGGACC TAGAATCGTCTCTCTTTCCAGCTCTCTTCATGTACTGGGAGCTGTAATGGTTACCTTTTCTATGTTGTGT ATTCAATAAACACACAGTAAATACCTCAAAAAAAAAAAAAAA
Restriction Sites RsrII-NotI |
ACCN BC006878
Insert Size 1002 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC006878, AAH06878
RefSeq Size 1372 bp
RefSeq ORF 1002 bp
Locus ID 13036
Cytogenetics 9 47.4 cM
Summary This gene encodes a member of the peptidase C1 (papain) family of cysteine proteases. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate multiple protein products. These products include the cathepsin H mini, heavy, and light chains. In rat and human, these three chains can associate to form the mature enzyme, which has both aminopeptidase and endopeptidase activities. Homozygous knockout mice for this gene exhibit impaired lung surfactant processing and reduced tumorigenesis in a pancreatic cancer model. Multiple pseudogenes of this gene have been identified in the genome. provided by RefSeq, Aug 2015
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