Ctu1 (NM_145582) Mouse Untagged Clone

CAT#: MC200291

Ctu1 (untagged) - Mouse cytosolic thiouridylase subunit 1 homolog (S. pombe) (Ctu1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ctu1
• Synonyms: Atpbd3; BC005752
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC005752 sequence for NM_145582
  CAGGCTTCCGCGTGGCAGGCAGAGTACTCAGCTCGTTCCACTCAGTGTAGACCTCTCAGACGACCCAGTT CTCTTTCAAGATGCCCGCCCCGACGTGCTTCTCCTGTCACAAGACCCGTGCTGCCCTCCGTCGCCCACGT TCTGGGCAGGCGCTGTGCGGCTCCTGCTTCTGTGCTGCCTTTGAGGCTGAGGTGCTACATACAGTGCTTG CCGGGCACCTGCTGCCGCCGGGCGCTGTAGTGGCCGTGGGTGCCTCTGGTGGCAAGGACTCCACAGTGCT GGCACACGTGCTGCGTGAGCTTGCGCCCCGCCTGGGCATCACCCTGCACCTGGTGGCGGTGGATGAGGGC ATCGGCGGCTACCGGGACGCCGCGCTGGAGGCAGTGCGCAGTCAGGCGGCGCGCTGGGAGCTGCCGCTCA CCATCGTGGCCTACGAAGATCTCTTCGGGGGCTGGACGATGGACGCCGTGGCCCGCAGCACCGCAGGCTC CGGCCGCAGTCGCTCCTGCTGCACCTTCTGCGGGGTGCTGCGGAGGCGCGCGCTGGAGGAGGGTGCGCGC CTTGTGGGAGCTACACATATTGTGACAGGCCACAACGCTGACGACATGGCTGAGACGGTGCTCATGAACT TCCTGCGTGGTGATGCTGGGAGGCTGGCGAGGGGTGGGGTGCTGGGCTCCACGGGCGAGGGGTGCGCACT TCCCAGGTGCCGGCCTCTGCAGTTTGCCTCGCAGAAGGAGGTGGTGCTGTATGCGCACTTCCGCCATCTC CGCTACTTTTCCGAGGAGTGCGTGTATGCCCCTGAGGCCTTCCGTGGTCATGCCCGGGACCTGCTTAAGC TCCTGGAGGCGGCCAGACCATCAGCCGTGCTGGACCTCGTGCACTCGGCAGAGCGCCTAGCTCTGGCTCC GGCAGCAAAGCCCCCTCCTCCAGGCACCTGCTCCCGCTGCGGAGCCCTGGCCAGCCACAAGCTCTGCCAG GCCTGTGCCCTCCTGGATGGCCTCAACCGAGGCCTGCCGCGCCTGGCCATCGGCAAGGGCCGCCGTGTGC TGCAAGTTGAGCCTCCACAGCCTGGGAACCCAAGCCTAGTCACCAGTGACCCTGTGGCCCCAGCAGGACC CTGCACCTGCAAGCAACCGAAAGATAAAGCCAACCCATGCGGCAATGGAGGGGACCGGGCAGGAGCCACC TGTGTATCGCAGTGTGACCTGAGCCCCGGGAATGGAGAGGACCGGGCAGGAGCCACCTGTGTATCGCAGA GGGACCTGAGCCTTGGGAATGGAGGGGACCGGGCAGGAGCCACCTGTGTATCGCAGTGTGACCTGAGCCC CGTGGCGGAGTGACCTGAGCTCTGCACTCTACATTGCCCTGTGTCCGGGGAAGTGATTGTACCCCACTGC CCTGCAACTGTGGAGTAATCTATACACTGTGAAGTGATTTCCCTTCAGTGCACGGCACCTTGGGACTGAC CTGTTTCCGTGTGGAGTGGCTGTACCCTGCAGAGTCGCTTGAGCCCTGCAGATTATTTAAAGTGTCCAGT GCCCTGCAACCTGAGCCCCGTGTCCTACCTGTACCCCATGGAAGGATGGGCCCTGTAGAGCAACATGAAC CTTGAAGTATAGGGCGCTCTAGGCACAGCTACCAGCTCTAAGAGAGAAGGGACCAACACCCATGCATTCC CGCACACCTCTGTGGGCTCAGAGAGCCCTGGCCCTCCTTTTCTGTTTTCCCAAGTCAGGTAAACAAAGTC ACATGCGTACTAGGAATATTCACAATCGTCACTATACCCGTCTCATCCAGGCACAGCTCTCGCACCTACG GGAACTGGAGAAAGTTTGTTCTGAACCAGTTGGGACCTTCCATGGGCGGGTATGTGAATTGAGGTTGCCT GGGAAACTGCCGTGGGAGCAGTTACACAAACGTTTACAGCCCTAGGACAATAGAAAGTCATTAATCAAGA CATTGGTCAAGTATGTCGGTGTGAACTGCAGGTAGACAGGTTATAGCAAAGCTGAAATCCCTTCTGTCAG TCTGAGGAACTATCTGATGACTTTCTTAGCTTGTGGGTCTTTGGAAGCCAGTGGTCTGCTGAGCTGATAG GTTCTGAACCAAATTTGTAGTTTTCACATGGTTGTTGAGTTAGTGTCCTGGCTGGCCATTTTTTGGTTTT GTTTTGTCAACTTTACACAGGCTGGGGTCATCTGAGAAAATGCTTCCATAAGATTGGCCTACAGGCAAGC TTGTGGGGCATTTTCTGATTGATGATTGATGTGGAAGGGCTCAGCCCACTGTGGGCGGTCCAGTCCCTAG GCAGGTGATCCTGGATACTATAAGAAAGCAGGCTGAGTAAGCCAGTCAGTAGTAGTGTTCCCTCATGGCC TCTGCCTCAGTTCCTGCTGACTTCCCTCAGTAATGGAGTGTGACCTAAAATGTAAGTTGAAATAAAACTT TTTCTCGAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_145582
• Insert Size: 1263 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC005752, AAH05752
• RefSeq Size: 2476 bp
• RefSeq ORF: 1263 bp
• Locus ID: 233189
• UniProt ID: Q99J10
• Cytogenetics: 7 B3
• Gene Summary: Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position.[UniProtKB/Swiss-Prot Function]