Edem2 (NM_145537) Mouse Untagged Clone

CAT#: MC200261

Edem2 (untagged) - Mouse ER degradation enhancer, mannosidase alpha-like 2 (Edem2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Edem2
• Synonyms: 9530090G24Rik; AI327354
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC008268 sequence for NM_145537
  GTGGCGGTGGCTGCAGCTGCTGCGGCGGCACTGACAGGACACGAGCTCTATGCCTTTCCGGCTACTCATC CCGCTCGGCCTCGTGTGCGTGCTGCTACCCCTGCACCATGGTGCGCCAGGCCCCGACGGCACCGCGCCCG ACCCCGCCCACTACAGGGAGCGAGTCAAGGCCATGTTCTACCACGCCTACGACAGTTACCTGGAAAATGC CTTTCCCTACGATGAGCTGAGACCTCTCACCTGTGATGGGCACGACACCTGGGGCAGTTTTTCTCTGACG CTAATTGATGCCCTGGACACCTTGCTGATTTTGGGGAATACCTCTGAATTCCAAAGAGTAGTGGAGGTTC TTCAGGACAATGTGGACTTTGATATCGACGTGAACGCCTCTGTGTTCGAAACCAACATCCGAGTGGTAGG AGGACTCCTTTCTGCTCATCTCTTGTCAAAGAAGGCCGGTGTGGAGGTGGAAGCCGGGTGGCCTTGTTCT GGGCCTCTCCTCAGGATGGCGGAGGAAGCAGCCCGGAAACTCCTCCCAGCATTTCAGACCCCCACGGGCA TGCCCTATGGAACTGTGAACCTGCTTCATGGCGTGAACCCTGGAGAGACCCCTGTTACCTGTACAGCAGG GATTGGGACCTTCATTGTGGAATTTGCTACCCTGAGCAGCCTCACCGGTGACCCTGTGTTTGAAGATGTG GCCAGAGTGGCCCTGATGCGTCTCTGGGAGAGCCGGTCAGATATCGGGCTGGTTGGCAATCACATCGATG TGCTCACTGGCAAGTGGGTGGCCCAGGATGCAGGCATCGGGGCTGGTGTGGATTCCTATTTTGAGTACCT GGTAAAAGGAGCCATCCTTCTTCAGGATAAGAAACTCATGGCCATGTTCCTAGAGTACAACAAAGCCATC CGGAATTACACGCACTTCGATGACTGGTACCTCTGGGTGCAGATGTACAAGGGGACCGTGTCAATGCCTG TCTTCCAGTCCTTGGAGGCCTACTGGCCTGGCCTTCAGAGCCTTATTGGAGACATTGACAATGCCATGAG GACCTTTCTCAACTACTACACTGTATGGAAGCAGTTTGGGGGGCTGCCAGAATTCTACAACATTCCTCAG GGATACACAGTGGAGAAGCGAGAAGGCTACCCACTCCGACCGGAGCTCATCGAGAGCGCAATGTACCTCT ACCGCGCCACTGGGGATCCCACTCTCTTAGAACTTGGGAGAGACGCTGTGGAATCCATTGAGAAAATCAG CAAGGTGGAGTGTGGATTTGCCACGATCAAGGACCTCCGGGACCACAAGCTTGACAACCGCATGGAGTCT TTCTTCCTGGCTGAAACTGTGAAATACCTCTATCTTCTGTTCCACCCAAACAACTTCATCCACAACAATG GCTCCACCTTTGACTCTGTGATGACTCCCCATGGGGAGTGCATCCTAGGAGCCGGGGGCTACATCTTCAA CACGGAGGCTCACCCCATTGACCCTGCTGCCTTGCACTGCTGCCGGAGGCTCAAGGAGGAGCAGTGGGAA GTGGAGGACTTGATAAAGGAGTTCTACTCCCTCAAACAGAGCAGGCCAAAGAGAGCTCAGAGGAAGACAG TGAGGTCGGGGCCCTGGGAGCCCCAGTCAGGGCCAGCCACACTCTTCTCACCTGCAAACCAGCCCAGGGA GAAGCAGCCTGCCCGGCAGCGAACCCCGCTTCTCAGCTGCCCCAGCCAGCCCTTCACCTCTAAGCTGGCA TTGCTAGGCCAGGTTTTCCTAGACTCCTCATAGCCACTGGATAATTTTCTAATTTTTATTTTTTTGAGAC TAAGCTCTAATAATAAATCTGCTTTTGGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_145537
• Insert Size: 1734 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC008268, AAH08268
• RefSeq Size: 1926 bp
• RefSeq ORF: 1734 bp
• Locus ID: 108687
• UniProt ID: Q8BJT9
• Cytogenetics: 2 H1
• Gene Summary: Involved in the endoplasmic reticulum-associated degradation (ERAD) pathway that targets misfolded glycoproteins for degradation in an N-glycan-dependent manner (PubMed:15579471, PubMed:25655076). May initiate ERAD by promoting the first mannose trimming step of ERAD substrates, from Man9GlcNAc2 to Man8GlcNAc2 (By similarity). Seems to recognize and bind to exposed hydrophobic regions in target proteins (PubMed:25655076).[UniProtKB/Swiss-Prot Function]