Ifit1 (NM_008331) Mouse Untagged Clone

CAT#: MC200183

Ifit1 (untagged) - Mouse interferon-induced protein with tetratricopeptide repeats 1 (Ifit1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ifit1
• Synonyms: Ifi56; ISG56; P56
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC003768 sequence for NM_008331
  CCCACGCGTCCGGGCAGGTTTCTGAGGAGTTCTGCTCTGCTGAAAACCCAGAGAACAGCTACCACCTTTA CAGCAACCATGGGAGAGAATGCTGATGGTGACCAGGTCATGGAGAATCTGCTTCAGCTGAGATGTCACTT CACATGGAAGCTGCTATTTGAAAATAATGACATACCTGATTTGGAAGTGAGAATCTCAGAGCAGGTCCAG TTCCTTGACATCAAGAACCCATTGGGGATGCACAACCTCCTGGCCTACGTGAGGCACCTGAAAGGCCAGC AGGACGAAGCCCTGCAGAGCTTGAAAGAAGCTGAAGCCTTGATCCAGAGCGAGCAGCTGAGCAAGAGAAG CCTGGCGACCTGGGGCAACTGTGCCTGGCTGCATTACCACAGGGGCAGCTTGGCAGAAGCCCAGATCTAC CTGGACAAGGTGGAGAAGGTGTGCAAGGAATTTTCAAGTCCCTTCCGCTACAGGCTGGAGTGTGCTGAGA TGGACTGTGAGGAAGGCTGGGCCTTGCTGAAGTGTGGAGGAGGAAATTATAAACAAGCCATGGCCTGCTT TGCGAAGGCTCTGAAAGTGGAGCCAGAAAACCCTGAGTACAACACTGGCTATGCAGTCGTAGCCTATCGC CAAGATTTAGATGACAACTTTATTTCTCTAGAACCTTTGAGGAAGGCTGTCCGGTTAAATCCAGAAGATC CATACCTTAAAGTTCTCCTTGCCCTAAAGCTTCAGGATTTAGGAGAACATGTTGAAGCAGAAGCACACAT TGAAGAAGCCCTCAGCAGCACATCTTGCCAAAGCTATGTCATTCGCTATGCAGCCAAATATTTCCGTAGG AAACATCGCGTAGACAAAGCTCTTCATCTTCTAAACAGGGCCTTGCAGGCATCACCTTCCTCTGGCTACT TACATTATCAAAAAGGGCTCTGCTACAAGCAACAAATCTCCCAACTGAGGACATCCCGAAACAGGCAGCC CAGAAGGCAGGACAATGTGCAAGAATTGGCACAACAGGCCATTCATGAATTTCAAGAGACTTTGAAACTG AGGCCCACATTTGAGATGGCCTATGTTTGCATGGCTGAAGTGCAGGCAGAAATTCACCAGTATGAAGAAG CAGAGAGAAATTTCCAGAAGGCACTGAACAACAAGACCCTCGTGGCTCACATAGAGCAGGATATTCACCT CCGCTATGGCCGTTTCCTACAGTTTCATAAGCAGTCAGAAGACAAGGCAATCACCCTCTACTTAAAAGGT CTAAAAGTGGAAGAGAAGTCCTTTGCTTGGAGGAAACTACTGACTGCTTTGGAGAAAGTGGCTGAAAGAC GTGTTTGTCAGAATGTTCATCTTGTAGAGAGTACCAGCCTTCTTGGGCTAGTCTACAAACTGAAAGGACA AGAGAAAAATGCTCTGTTTTACTATGAGAAGGCACTGAGGCTCACTGGGGAAATGAACCCTGCATTCTGA ATGCAGCTCACCTCTGTGACGTTAATATACTCACAACCAAGTGTTCCAATGCTCCTTCCTGATTTCTTTT CCCAGGTCTGTTTTGTTGTTGTTGTTGTTCGTTTTCTTTCTGAAATGCCAAGTAGCAAGGTACATTTTCT CCTGTGACTTCTTTCTGGTCTTTCTGACCCTGTCTAGCAGGCAATTCCATCCCAAGGATACATACTCACT TAAAGATCACAACTGATTCCTGCTGTTTTGGACTCCTGTGAGCTGTAGAATCACACAACCTTTTTCCTTA TGTCTGTGCCTTTGGAACAGATATCTTCCACCCTTTGCTAATGTCCTGGACAAAAAAGTCTTTGCCAAAG AAGGTATTTTTTTTTTAATGTCACCGGAAATGCTTATTACCATACAATTTCAGAGCAAAACAAATGAGCA AAGAAGAAAGTTTGAAAGCAAAAGAGGTCACACATTACTGGAAAGATACTAAGAACCAAGAAGACTTTTG GCTTACTAGAGAATTAAAATGAAAACCCTCCTTAGGTAGTAAAGTCTGAAAATTACATAAATAACTTCTT TCAATATTTATATGCTAAAAGTATTCATTGAAAGTGACCAAAATAGTTAAAGCCAAAACCAACAAAAAAA AGAAAGAAAGAAAGAAAGAGAGAAAAGAAAAGAAAAGAAAAGAAAAGAAAAGAAAAGAAAAAAAAGAAAA GAAAAGAAAAGAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_008331
• Insert Size: 1392 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC003768, AAH03768
• RefSeq Size: 2196 bp
• RefSeq ORF: 1392 bp
• Locus ID: 15957
• UniProt ID: Q64282
• Cytogenetics: 19 C1
• Gene Summary: Interferon-induced antiviral RNA-binding protein that specifically binds single-stranded RNA bearing a 5'-triphosphate group (PPP-RNA), thereby acting as a sensor of viral single-stranded RNAs and inhibiting expression of viral messenger RNAs. Single-stranded PPP-RNAs, which lack 2'-O-methylation of the 5' cap and bear a 5'-triphosphate group instead, are specific from viruses, providing a molecular signature to distinguish between self and non-self mRNAs by the host during viral infection. Directly binds PPP-RNA in a non-sequence-specific manner. Viruses evolved several ways to evade this restriction system such as encoding their own 2'-O-methylase for their mRNAs or by stealing host cap containing the 2'-O-methylation (cap snatching mechanism).[UniProtKB/Swiss-Prot Function]