Clp1 (NM_133840) Mouse Untagged Clone

CAT#: MC200178

Clp1 (untagged) - Mouse CLP1, cleavage and polyadenylation factor I subunit, homolog (S. cerevisiae) (Clp1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Clp1
• Synonyms: AI462438; Heab
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC003237 sequence for NM_133840
  GGTGAACGTTATTCGTGGTTTGCTGAGGTCCGAGGTTTGCTGAGGTCGGAGGTTTCCAGAGGTCCCAGGT TTCCGTGAAGCTGGTTTTGAGTCCGTGCCCGTATAGGCACGTCAGCTTTACCGGAAAGATGAGCGAGGAA TCCAATGATGACAAGAAGCCAACGACTAAGTTTGAACTAGAGCGAGAAACAGAGCTTCGATTTGAGGTGG AGGCCTCTCAATCGGTTCAGTTGGAGTTGTTGGCTGGCATGGCGGAGATCTTTGGCACAGAGCTGACCAG AAACAAGAAATTCACTTTTGATGCTGGTGCCAAGGTGGCTGTTTTCACTTGGCATGGCTGTTCTCTGCAG TTGAGTGGCCGGACTGAGGTGGCTTATGTCTCTAAGGACACCCCTATGTTGCTTTACCTCAACACTCATA CAGCCTTGGAGCAGATGCGGAGGCAGGCAGAAAAGGAAGAAGAGAGAGGTCCCCGAGTGATGGTAGTAGG TCCTACTGATGTGGGCAAGTCCACTGTGTGTCGACTGCTACTCAACTACGCAGTGCGTTTGGGCCGCCGT CCTACTTACGTGGAGTTAGATGTAGGCCAGGGCTCTGTGTCCATCCCTGGTACCATGGGGGCCCTGTACA TTGAGCGTCCAGCAGATGTGGAAGAAGGTTTCTCCATCCAGGCCCCTCTTGTGTATCATTTTGGCTCCAC CACGCCTGGCACCAACATCAAGCTTTACAATAAGATTACATCTCGCTTAGCAGATGTGTTCAACCAAAGG TGTGAAGTGAACAGAAGGGCTTCTGTGAGTGGCTGTGTCATCAACACCTGTGGCTGGGTCAAGGGCTATG GTTACCAAGCCCTGGTGCACGCAGCCTCGGCCTTTGAGGTGGATGTGGTTGTGGTTCTGGATCAAGAGCG ACTGTACAACGAATTGAAAAGGGACCTGCCTCATTTTGTTCGAACTGTGTTGCTTCCAAAATCAGGGGGT GTGGTAGAACGTTCCAAGGACTTCCGGCGGGAATGTAGAGATGAACGAATCCGTGAGTATTTCTATGGAT TTCGAGGCTGTTTCTATCCCCATGCCTTCAACGTCAAATTTTCTGATGTGAAAATCTACAAAGTTGGAGC ACCCACCATTCCAGATTCCTGTTTACCTCTGGGTATGTCTCAGGAAGACAACCAGCTCAAGTTAGTGCCT GTCACCCCTGGTAGAGATATGGTGCACCATCTCCTGAGTGTGAGCACTGCTGAGGGCACAGAAGAGAACC TTTCTGAGACCAGTGTGGCTGGATTCATTGTTGTGACCAGTGTGGATGTGGAACACCAAGTGTTTACTGT CCTGTCTCCAGCCCCCCGCCCATTGCCTAAGAACTTTCTTCTCATCATGGATATCCGGTTCATGGATCTC AAGTAGTAATTGCAAGAAGCCTCACTTTCTGGGCCATAGAGTTCTGGTCATCACCAAAGGCAGATGGAAT GGACTATAGCAATCTTATTAAGGCAGCTGACAAGGCCCAACTAGCAGAAAGCACATTTGCTACCTCTTGA AACTGGTAGTAGCCAGACTCCTGGCTATTATTTGGTTCATTTACTCTGAACCAAAAGGAGACAGCTCAAA AAGAAAATCTGAATATTTTTTTAAGATCACCTAAGGTACCATTTTAAAGATTCCAAGACCCTAGAAAATT CCACGTCTTCCACATGTGCTTCCATGTGGGTTTTCTTGTTACCACTTTCTAGTATCTCTGTATAGTATTT GCTATATCATTTAACCAGGATGAAAGTGAGAGAGTTCAAACTTGAAAACAATTATTTTAATAAATATGAT TGTGATAGAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_133840
• Insert Size: 1278 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC003237, AAH03237
• RefSeq Size: 1844 bp
• RefSeq ORF: 1278 bp
• Locus ID: 98985
• UniProt ID: Q99LI9
• Cytogenetics: 2 D
• Gene Summary: Polynucleotide kinase that can phosphorylate the 5'-hydroxyl groups of double-stranded RNA (dsRNA), single-stranded RNA (ssRNA), double-stranded DNA (dsDNA) and double-stranded DNA:RNA hybrids. dsRNA is phosphorylated more efficiently than dsDNA, and the RNA component of a DNA:RNA hybrid is phosphorylated more efficiently than the DNA component. Plays a key role in both tRNA splicing and mRNA 3'-end formation. Component of the tRNA splicing endonuclease complex: phosphorylates the 5'-terminus of the tRNA 3'-exon during tRNA splicing; this phosphorylation event is a prerequisite for the subsequent ligation of the two exon halves and the production of a mature tRNA (PubMed:23474986, PubMed:24766809). Its role in tRNA splicing and maturation is required for cerebellar development (PubMed:24766809). Component of the pre-mRNA cleavage complex II (CF-II), which seems to be required for mRNA 3'-end formation. Also phosphorylates the 5'-terminus of exogenously introduced short interfering RNAs (siRNAs), which is a necessary prerequisite for their incorporation into the RNA-induced silencing complex (RISC). However, endogenous siRNAs and microRNAs (miRNAs) that are produced by the cleavage of dsRNA precursors by DICER1 already contain a 5'-phosphate group, so this protein may be dispensible for normal RNA-mediated gene silencing.[UniProtKB/Swiss-Prot Function]