Pbx1 (BC002244) Mouse Untagged Clone

CAT#: MC200105

Pbx1 (untagged) - Mouse pre B-cell leukemia transcription factor 1 (cDNA clone MGC:7546 IMAGE:3492658), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pbx1
• Synonyms: 2310056B04Rik; D230003C07Rik; Pbx-1
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC002244
  GCGGGAGGAAGCAGGACATCGGGGACATTTTACAGCAAATTATGACCATCACAGACCAGAGTTTGGATGA AGCGCAGGCCAGAAAACATGCTTTAAACTGCCACAGAATGAAGCCTGCCTTGTTTAATGTGTTGTGTGAA ATCAAAGAAAAAACAGTTTTGAGTATTCGGGGAGCCCAAGAAGAGGAGCCCACAGACCCCCAGCTCATGC GACTGGACAACATGCTGCTAGCAGAAGGGGTGGCGGGGCCTGAGAAGGGCGGAGGCTCGGCAGCGGCGGC GGCGGCAGCGGCAGCTTCTGGGGGTGCAGGTTCAGACAACTCAGTGGAGCATTCCGACTACAGAGCCAAA CTCTCACAGATCAGACAAATCTACCACACAGAGCTGGAGAAGTATGAGCAGGCATGCAATGAATTCACCA CCCACGTGATGAACCTCCTTCGAGAGCAAAGCCGGACCAGGCCCATCTCTCCGAAGGAGATCGAGCGGAT GGTGAGCATCATCCACCGCAAGTTCAGCTCCATCCAGATGCAGCTGAAACAGAGCACGTGCGAGGCCGTC ATGATCCTGCGCTCCCGGTTCCTGGATGCGAGGCGGAAGAGACGGAATTTCAACAAGCAAGCCACAGAAA TTCTGAATGAATATTTCTATTCCCATCTCAGCAACCCTTACCCCAGTGAGGAAGCCAAAGAGGAGTTAGC CAAGAAGTGCGGCATCACAGTCTCCCAGGTATCAAACTGGTTTGGAAATAAGCGAATCCGGTACAAGAAG AACATAGGTAAATTTCAAGAGGAAGCCAATATTTATGCTGCCAAAACGGCTGTCACAGCCACCAATGTGT CAGCCCATGGAAGCCAAGCTAACTCGCCCTCTACTCCCAACTCAGCGGGTGGATACCCTTCGCCATGTTA TCAGCCAGACAGGAGGATACAGTGACGGACTCGCAGCCAGTCAGATGTACAGTCCGCAGGGCATCAGTGC TAATGGAGGTTGGCAGGATGCTACTACCCCTTCATCAGTGACCTCCCCTACAGAAGGCCCTGGCAGTGTT CACTCTGATACCTCCAACTGATCTCCCAGCAATCGCATCCCGGCTGACCCTGTGCCGCAGTTGGGGCAGG GCCAGGAGGGAGGGTTTCTCTCCCAACGCTGAAGCGGTCAGACTGGAGGTCGAAGCAATCAGCAAACACA ATAAGAGTCTCCTTCTCTTCTCTTCTTTGGGATGCTATTTCAGCCAATCTGGACACTTCTTTATACTCTC TTCCCTTTTTTTCTGGGTAGAAGCCACCCTTCCCTGCCTCCAGCTGTCAGACTGGTTTCTGTCATCTTCC CTGGCCCCTGCTCTATGTTATAGACGCCCCGGTCCCTGCCCTCTATGACTTCACTGAAGGATTTTTTTTT CTTCCACAATTAGAGGAATTTAAAAAGGAAAAACAAATTACAAAGAAAACAATAAACGCATTTGTATGTT TTCAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: BC002244
• Insert Size: 729 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC002244, AAH02244
• RefSeq Size: 1491 bp
• RefSeq ORF: 729 bp
• Locus ID: 18514
• Cytogenetics: 1 75.95 cM
• Gene Summary: This gene encodes a homeobox protein that belongs to the three-amino-acid loop extension/Pre-B cell leukemia transcription factor (TALE/PBX) family of proteins. The encoded protein is involved in several biological processes during embryogenesis including steroidogenesis, sexual development and the maintenance of hematopoietic stem cells. This protein functions in the development of several organ systems and plays a role in skeletal patterning and programming. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014]