Spp1 (BC002113) Mouse Untagged Clone

CAT#: MC200083

Spp1 (untagged) - Mouse secreted phosphoprotein 1 (cDNA clone MGC:6613 IMAGE:3489075), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Spp1
• Synonyms: minopontin, BNSP, BSPI, ETA-1, OP
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC002113
  CCCACGCGTCCGCGGACGCGTGGGCCTTGCTTGGGTTTGCAGTCTTCTGCGGCAGGCATTCTCGGAGGAA ACCAGCCAAGGACTAACTACGACCATGAGATTGGCAGTGATTTGCTTTTGCCTATTTGGCATTGCCTCCT CCCTCCCGGTGAAAGTGACTGATTCTGGCAGCTCAGAGGAGAAGAAGCTTTACAGCCTGCACCCAGATCC TATAGCCACATGGCTGGTGCCTGACCCATCTCAGAAGCAGAATCTCCTTGCGCCACAGAATGCTGTGTCC TCTGAAGAAAAGGATGACTTTAAGCAAGAAACTCTTCCAAGCAATTCCAATGAAAGCCATGACCACATGG ACGACGATGATGACGATGATGATGACGATGGAGACCATGCAGAGAACGAGGATTCTGTGGACTCGGATGA ATCTGACGAATCTCACCATTCCGATGAGTCTGATGAGACCTTCACTGCTAGTACACAAGCAGACACTTTC ACTCCAATCGTCCCTACAGTCGATGTCCCCGACGGCCGAGGTGATAGCTTGGCTTATGGACTGAGGTCCA AGTCTAGGAGTTTCCAGGTTTCTGATGAACAGTATCCTGATGCCACATATGAGGACCTAACCTCTCACAT GAAAAGCGGTGAGTCTAAGGAGTCCCTCAATGTCATCCCTGTTGCCCAGCTTCTGAGCATGCCCTCTGAT CAGGACAACAACGGAAGGGGCAGCCATGAGTCAAGTCAGCTGGATGAACCAAGTCTGGAAACACACAGCC TTGAACATTCCAAAGAGAGCCACGAGAGTGCCGATCAGTCGGATGTGATCGATAGTCAAGCAAGTTCCAA AGCCAGCCTGGAACATCAGAGCCACAAGTTTCACAGCCACAAGGACAAGCTAGTCCTAGACCCTAAGAGT AAGGAAGATGATAGGCATCTGAAATTCCGCATTTCTCATGAATTAGAGAGTTCATCTTCTGAGGTCAACT AAAGAAGAGGCAAAAACACAGTTCCTTACTTTGCATTTAGTAAAAACAAGAAAAAGTGTTAGTGAGGGTT AAGCAGGAATACTAACTGCTCATTTCTCAGTTCAGTGGATATATGTATGTAGAGAAAGAGAGAGGTAATA TTTTGGGCTCTTAGCTTAGTCTGTTGTTTCATGCAAACACCGTTGTAACCAAAAGCTTCTGCACTTTGCT TCTCTTCTTCCTGTACAAGAAATGCAAACGGCCACTGCATTTTAATGATTGTTATTCTTTTATGAATAAA ATGTATGTAGAAACAAGCAAATTTACTGAAACAAGCAGAATTAAAAGAAACTGTAACAGTCTATGTCACT ATACCCTTTTAGTTTTATAATTAGCATATATTTTGTTGTGATTATTTTTTTTTGTTGGTGTGAATAAATC TTGTAACGAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: BC002113
• Insert Size: 888 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC002113, AAH02113
• RefSeq Size: 1428 bp
• RefSeq ORF: 888 bp
• Locus ID: 20750
• Cytogenetics: 5 50.68 cM
• Gene Summary: Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.[UniProtKB/Swiss-Prot Function]