Klf10 (NM_013692) Mouse Untagged Clone

CAT#: MC200029

Klf10 (untagged) - Mouse Kruppel-like factor 10 (Klf10), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Klf10
• Synonyms: AI115143; Egral; EGR[a]; Gdnfif; mGIF; Tieg; TIEG-1; Tieg1
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC003316 sequence for NM_013692
  GGCTAACTAAGCAGCCAGCAGCCAGGCAGCTCGCGACCTGCGGCCAGGCAGCCAACCATGCTCAACTTCG GCGCTTCTCTCCAGCAAGCTTCGGAGGGGAAAATGGAACTAATTTCTGAAAAGCCCAGAGAGGGGATGCA TCCCTGGGACAAAGCTGAGCAGAGTGACTTTGAAGCGGTGGAAGCGCTCATGTCCATGAGCTGCGACTGG AAGTCTCATTTCAAGAAATACCTTGAAAACAGGCCTGTCACACCAGTGTCTGATACCTCCGAGGATGACA GCTTGCTTCCAGGGACGCCTGACCTTCAGACAGTCCCAGCATTTTGTTTAACGCCACCTTACAGCCCCTC TGACTTCGAACCCTCCCAAGGGTCAAATCTGACTGCATCAGCGCCATCTACTGGCCACTTCAAATCTTTC TCCGATGCTGCCAAGCCTCCAGGCGCCACTCCTTTCAAAGAGGAGGAAAAGAATCCTTTAGCTGCCCCTC CTCTTCCTAAGGCTCAAGCCACCAGTGTCATCCGTCACACAGCTGATGCCCAACTGTGCAACCACCAGTC CTGCCCCGTGAAAGCAGCTAGCATCCTCAACTATCAGGACAATTCTTTCCGGAGAAGAACCCACGGAAAT GTTGAGGCTACTCGAAAGAACATACCCTGTGCTGCAGTGTCACCAAACAGATCCAAGCCTGAGCCCAGCA CAGTGTCCGATGGTGATGAGAAGGCGGGCGCTGCACTATATGACTTTGCTGTGCCTTCCTCAGAGACAGT AATTTGTAGGTCTCAGCCAGCTCCTTCGTCCCCAGTGCAGAAGTCAGTACTGGTGTCTTCACCTACAGTA TCCACTGGGGGAGTGCCACCCCTGCCTGTCATCTGCCAGATGGTTCCCCTTCCTGCCAACAACTCTCTTG TTAGCACAGTTGTCCCCAGCACTCCTCCTAGCCAGCCACCAGCTGTCTGCTCACCTGTGTTGTTCATGGG CACTCAGGTGCCTGAGGGCACCGTCGTGTTTGTGGTACCCCAGCCCGTTGTGCAGAGCCCAAGGCCTCCA GTGGTGAGCCCCAGTGGCACCAGACTGTCTCCCATTGCCCCTGCTCCTGGATTCTCTCCTTCAGCAGCAA GGGTCACTCCTCAGATTGACTCGTCCAGAGTAAGAAGTCACATCTGTAGCCACCCAGGGTGTGGCAAGAC TTACTTTAAAAGTTCCCATCTGAAGGCCCACGTGAGGACACACACAGGGGAAAAACCTTTCAGCTGCAGC TGGAAAGGCTGTGAAAGGAGGTTTGCTCGCTCCGATGAACTGTCCAGACACCGGCGGACACACACAGGTG AGAAGAAGTTTGCCTGTCCCATGTGTGACCGTCGGTTTATGAGGAGCGACCATTTAACCAAGCATGCCCG ACGCCACCTATCAGCCAAGAAGCTGCCAAACTGGCAAATGGAAGTTAGCAAGTTAAATGACATTGCTCTG CCTCCGACCCCTGCTTCCGCACAGTGACGGCCAGAAGATGGAGACGCAGAATAAACTTTGGTCAGAGTCA GGAGCCAGTGATGGTGTCAAGTGCTTCTGCAAGGCTGTGGCCCTCCAAAAGGGCCTAAAGTAGAAGCCCT GGCCTGGGGGAGGCCCCGCCTGGGTGAAATGACAAGAAGTGCTTCAGCCACAGGCAGGTCACAGAGGACA GGGCTCAGTTCTTACCACAGAGAGAGAGGAGAACCCTTTTATTCCTCCCTTATTTTAGTCTGGAAAGTTT CGGCTGAGGTGAGCGCAGCACAGGTTTTGAATCACATACACATTGGGGACTTTGTTTTTGCCATTTATAC TTGAGACCAGCTTTGCAGTGTGATTCTTTCAAAGGATTGGTTTCAAGAATATAGAGGCTGGAAATTACGG TACAGAAATGGAGCTAGAAAATGAGTTTGTGTTACACAGAGATGTCATCTTCTCCTAGAGTTATCTTGTT TCTTATTCCTAGTCTTTCCAGTCAAATCCGTGGATGTAGCTAAGTATATCTAAAACTCATTTTTCCACTA TTGTTGGTATTTGAAGTTGAACAGCTGTACATTGCTGTGGGGGAGCCAAAGGATTGGAACCCTCATTAAT TTAATTGCTTGGAAATGCAGCTAAAATTCTTCTTTGGCATTTTGTTTTGAAAGTTTAGGCATTTTACTCT ACTTTAGATTTTAGTTTGCTTGCAGTTTTTTGTGTAGATTTGAAAATTGTATACCAATGTGTTTTCTGTA GGCTTAAAATACACTGCACTTTGTTTAGAAAAAAATCTGGAGATGAAAATATGTATTATAAAGAAGAGAT GTCAAGAATTTGAGATAACTCCTTGAGAAAGTTGGCTTTATGTCATCAGCAAAGGACACTTAACGTCAAG CATACACTGTGGTTTTTTTGTTTTTTTGTTTTTTTTTTTCAAATTAGAAAGTTTAATGACCGTTACAGAT GGACAGTGTCTTTTTATTTATAGGAGTTTTTCAGGATGTCAGAGTAGATAGGTAGGAAAATTGTTATTAG AACATTCGCTTCTACCTTGAAAAGGATGTTAATGTGGTCATGTTCTTAGCACCACAGTGTCTGGGCATCT GGGAAACTCCGAGACTTTTTTAAAGTGTCATGATGTGATCACACCTGCAGTTTGGGGCATCGAATCCAGG GCCTTGCATGTCTTCTGTAAGAGCTCTCATCGCTGACCTGTATCCCCCGCAAGAGCAATGACTTTTGCTA ACAGTATTTCTTTTCTGTTGTAAAGTGGACAGATGATACACTTGGTCGCAAAGGTAAATTATTCAAAATC CACAGTGAAAACCTCACCACACTTTCCCATTTAAACTATTTCCATATCTCAGAGGTTTCTGACATGCAAA CTTGAACCCTTGAAAGAAGAGTTTTCTTAAAAATTATAAAAAATCACGAGTTACAATTTGCACAATATTT TTTGTTGAACTTTATACCTTGTTTACAATAAAGACTTTTCTTTGGTATAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_013692
• Insert Size: 1440 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC003316, AAH03316
• RefSeq Size: 3003 bp
• RefSeq ORF: 1440 bp
• Locus ID: 21847
• UniProt ID: O89091
• Cytogenetics: 15 B3.1
• Gene Summary: Transcriptional repressor which binds to the consensus sequence 5'-GGTGTG-3'. May play a role in the cell cycle regulation (By similarity). Plays a role in the regulation of the circadian clock; binds to the GC box sequence in the promoter of the core clock component ARTNL/BMAL1 and represses its transcriptional activity. Regulates the circadian expression of genes involved in lipogenesis, gluconeogenesis, and glycolysis in the liver. Represses the expression of PCK2, a rate-limiting step enzyme of gluconeogenesis.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.